cDNA Clone PHF2

• Plasmid: pBABE 3x FLAG PHF2
• Parent Plasmid: pBABE 3x FLAG
• Expressed: 3x FLAG PHF2
• Genbank: NM_005392.3
• Tag: 3FLAG
• Species: Human

Sequence of Insert

GGATCCATGGCGACGGTGCCCGTGTACTGCGTCTGCCGGCTCCCCTACGACGTTACCCGCTTCATGATCGAGTGCGACGCCTGCAAGGACTGGTTCCACGGCAGCTGTGTTGGGGTGGAAGAGGAGGAGGCGCCCGACATCGACATATACCACTGCCCAAACTGTGAGAAAACCCATGGGAAGTCCACCTTAAAGAAGAAGCGGACCTGGCACAAACACGGCCCGGGGCAAGCGCCTGACGTCAAGCCCGTGCAGAATGGCAGCCAGCTCTTCATCAAGGAGCTGCGGAGCCGGACCTTTCCCAGTGCTGAAGACGTGGTGGCCCGTGTGCCAGGAAGTCAGCTCACGCTGGGCTACATGGAGGAGCACGGCTTCACCGAGCCCATCCTCGTCCCTAAGAAAGACGGGCTGGGTCTGGCTGTCCCGGCCCCCACGTTCTATGTCAGTGACGTCGAGAACTACGTGGGGCCGGAACGGAGTGTGGATGTGACAGATGTCACCAAGCAGAAGGACTGCAAGATGAAGCTGAAGGAGTTTGTGGACTATTACTACAGCACCAACCGCAAGCGGGTCCTCAACGTCACCAACCTCGAGTTCTCTGACACCCGAATGTCCAGCTTCGTGGAGCCACCTGACATTGTAAAGAAACTGTCATGGGTAGAAAACTACTGGCCAGATGATGCATTGCTGGCCAAGCCCAAAGTGACCAAGTACTGCCTAATCTGCGTGAAGGACAGTTACACCGACTTCCACATCGACTCTGGGGGCGCCTCTGCCTGGTACCACGTGCTCAAGGGGGAGAAGACCTTCTATCTCATCAGGCCGGCCTCGGCCAACATCTCCCTGTATGAGCGCTGGCGGTCTGCCTCTAACCACAGCGAGATGTTCTTTGCTGACCAGGTCGACAAATGCTACAAGTGCATCGTCAAGCAGGGCCAGACCCTCTTCATCCCCTCAGGCTGGATCTACGCCACACTCACCCCTGTGGACTGCCTGGCCTTCGCGGGACATTTCCTCCACAGCCTGAGTGTGGAGATGCAGATGAGAGCATATGAAGTGGAAAGGAGGTTGAAACTGGGCAGCCTGACTCAGTTTCCCAACTTTGAAACTGCGTGCTGGTACATGGGGAAGCACCTGCTGGAGGCATTCAAAGGCTCTCACAAATCTGGGAAGCAGCTGCCCCCTCATCTAGTCCAAGGAGCTAAAATTCTCAATGGTGCTTTCCGATCGTGGACGAAGAAGCAGGCTTTGGCAGAGCATGAGGACGAGCTCCCGGAGCACTTCAAACCTTCACAGCTAATCAAAGACCTGGCCAAAGAGATCCGGCTCAGTGAGAATGCCTCCAAAGCCGTCCGACCGGAAGTGAATACTGTCGCCTCGTCAGATGAGGTGTGTGACGGGGACCGGGAGAAGGAGGAGCCCCCGTCTCCCATTGAGGCCACCCCGCCTCAATCCCTCCTGGAGAAAGTGTCCAAAAAAAAGACTCCCAAAACTGTGAAGATGCCCAAGCCATCCAAAATCCCCAAGCCCCCGAAGCCCCCTAAGCCCCCAAGGCCCCCCAAAACGCTGAAGCTCAAAGATGGAGGCAAGAAGAAAGGGAAGAAGTCCCGGGAGTCAGCCTCACCCACCATCCCCAACCTGGACCTGCTCGAAGCCCACACCAAGGAGGCACTGACCAAGATGGAGCCGCCCAAGAAGGGCAAGGCCACAAAGAGTGTCCTGAGTGTGCCCAACAAAGATGTGGTTCACATGCAGAATGATGTGGAGAGGCTGGAAATTCGAGAGCAAACCAAGAGCAAGTCAGAGGCCAAGTGGAAGTACAAGAACAGCAAACCTGACTCCTTACTGAAGATGGAAGAGGAGCAGAAGCTAGAGAAGTCGCCTCTAGCTGGAAACAAAGACAATAAGTTCTCTTTTTCTTTCTCCAACAAGAAACTCCTCGGCTCCAAGGCTCTCAGGCCCCCGACGAGCCCTGGTGTGTTCGGGGCCTTGCAGAACTTCAAGGAGGACAAGCCCAAGCCCGTGCGGGATGAGTATGAGTACGTGTCGGATGACGGTGAGCTCAAGATCGACGAGTTTCCCATCAGGAGGAAGAAAAACGCCCCGAAAAGGGACTTGTCCTTCTTGTTGGATAAGAAGGCTGTGCTGCCCACGCCTGTCACGAAGCCAAAGCTGGACTCGGCAGCGTACAAGCAGAGTGATGACTCCTCGGACGAGGGTTCGCTGCACATCGACACAGACACCAAGCCCGGCCGCAATGCCAGAGTCAAGAAGGAGAGTGGGAGCTCGGCAGCTGGCATCTTGGACCTGCTGCAGGCCAGTGAGGAGGTTGGCGCGCTGGAGTACAACCCCAGCAGCCAGCCCCCGGCCTCCCCCAGCACACAGGAAGCCATTCAGGGAATGCTGTCCATGGCCAACCTGCAGGCCTCCGACTCCTGCCTGCAGACCACGTGGGGAGCTGGCCAGGCCAAGGGGAGCTCGCTGGCTGCCCATGGTGCCCGGAAGAATGGGGGTGGCAGTGGCAAGAGTGCAGGCAAACGACTGCTGAAGAGGGCTGCCAAGAACAGTGTCGACCTGGACGACTACGAGGAAGAGCAGGACCACCTGGATGCCTGCTTCAAGGACTCAGACTACGTTTACCCCTCACTGGAGTCAGATGAAGACAACCCCATCTTTAAGTCCCGGTCGAAGAAAAGGAAAGGCTCAGACGACGCTCCCTACAGCCCAACAGCAAGGGTCGGCCCATCGGTGCCAAGACAGGACAGGCCTGTGCGTGAGGGTACACGGGTGGCTTCCATCGAGACCGGGCTGGCGGCTGCTGCAGCTAAGTTGTCCCAGCAGGAGGAGCAGAAAAGCAAGAAAAAAAAGAGTGCCAAGAGGAAGCTGACTCCTAACACCACCTCCCCTTCCACCTCCACCTCCATCTCTGCCGGCACCACCTCCACCTCCACCACGCCAGCCTCTACCACCCCGGCCTCCACCACCCCGGCCTCCACCAGCACGGCCAGCAGCCAGGCCTCGCAGGAGGGCAGCTCGCCAGAGCCCCCGCCTGAGTCGCATAGCAGCAGCCTGGCGGACCATGAGTACACAGCCGCTGGCACCTTCACCGGGGCCCAGGCTGGCCGCACCTCCCAGCCCATGGCCCCTGGGGTCTTTCTCACACAGAGGCGGCCCTCCGCATCGTCTCCAAACAACAACACCGCTGCCAAAGGAAAACGTACGAAAAAGGGCATGGCGACCGCCAAGCAGAGGCTTGGGAAAATTTTGAAAATTCATCGGAACGGGAAACTACTCCTTTAAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGSDYKDDDDKDYKDDDDKGSMATVPVYCVCRLPYDVTRFMIECDACKDWFHGSCVGVEEEEAPDIDIYHCPNCEKTHGKSTLKKKRTWHKHGPGQAPDVKPVQNGSQLFIKELRSRTFPSAEDVVARVPGSQLTLGYMEEHGFTEPILVPKKDGLGLAVPAPTFYVSDVENYVGPERSVDVTDVTKQKDCKMKLKEFVDYYYSTNRKRVLNVTNLEFSDTRMSSFVEPPDIVKKLSWVENYWPDDALLAKPKVTKYCLICVKDSYTDFHIDSGGASAWYHVLKGEKTFYLIRPASANISLYERWRSASNHSEMFFADQVDKCYKCIVKQGQTLFIPSGWIYATLTPVDCLAFAGHFLHSLSVEMQMRAYEVERRLKLGSLTQFPNFETACWYMGKHLLEAFKGSHKSGKQLPPHLVQGAKILNGAFRSWTKKQALAEHEDELPEHFKPSQLIKDLAKEIRLSENASKAVRPEVNTVASSDEVCDGDREKEEPPSPIEATPPQSLLEKVSKKKTPKTVKMPKPSKIPKPPKPPKPPRPPKTLKLKDGGKKKGKKSRESASPTIPNLDLLEAHTKEALTKMEPPKKGKATKSVLSVPNKDVVHMQNDVERLEIREQTKSKSEAKWKYKNSKPDSLLKMEEEQKLEKSPLAGNKDNKFSFSFSNKKLLGSKALRPPTSPGVFGALQNFKEDKPKPVRDEYEYVSDDGELKIDEFPIRRKKNAPKRDLSFLLDKKAVLPTPVTKPKLDSAAYKQSDDSSDEGSLHIDTDTKPGRNARVKKESGSSAAGILDLLQASEEVGALEYNPSSQPPASPSTQEAIQGMLSMANLQASDSCLQTTWGAGQAKGSSLAAHGARKNGGGSGKSAGKRLLKRAAKNSVDLDDYEEEQDHLDACFKDSDYVYPSLESDEDNPIFKSRSKKRKGSDDAPYSPTARVGPSVPRQDRPVREGTRVASIETGLAAAAAKLSQQEEQKSKKKKSAKRKLTPNTTSPSTSTSISAGTTSTSTTPASTTPASTTPASTSTASSQASQEGSSPEPPPESHSSSLADHEYTAAGTFTGAQAGRTSQPMAPGVFLTQRRPSASSPNNNTAAKGKRTKKGMATAKQRLGKILKIHRNGKLLL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU55486
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: extra Q after AA K729 (SNP), difference to ref sequenence
• Low Temp: No