cDNA Clone TEL1

• Plasmid: pGEX6P1 TEL1 (yeast) 221-442
• Parent Plasmid: pGEX6P1
• Expressed: GST TEL1 (yeast) 221-442
• Genbank: NP_009465.2
• Tag: GST
• Species: Yeast

Sequence of Insert

GGATCCGGAAATACGAGATTAATAATGTCATTAATAAATCAATTAATTTTGAAGTGCCATTGTTTTAGTGTTATTGATACGCTAATGCTTATAAAAGAAG
CATGGAGTTACAACCTGACAATTGGCTGTACTTCCAATGAGCTAGTACAAGACCAATTATCACTGTTTGATGTTATGTCAAGTGAACTAATGAACCATAA
ACTTCCTTATATGATTGGTCAAGAGAATTATGTTGAAGAGCTTCGGTCCGAATCTCTTGTATCTCTATACCGTGAGTACATTCTACTGCGCTTAAGTAAT
TATAAGCCTCAATTATTTACCGTAAACCATGTGGAATTCTCATATATTCGAGGTTCAAGGGATAAAAATTCATGGTTTGCATTACCTGATTTTAGACTTA
GAGATAGGGGAGGCAGATCGGTGTGGTTAAAAATACTCGGAATTACCAAATCATTGTTAACATATTTTGCATTGAACAGAAAAAATGAAAATTACTCATT
ATTATTTAAAAGAAGAAAATGTGATTCGGATATACCTTCTATCCTACGGATTTCTGACGATATGGACACATTTCTTATTCATCTTTTAGAGGAGAACAGC
TCACATGAGTTTGAAGTGCTAGGATTACAATTGTGCTCATTTTATGGAACTTTACAAGACTTCACTAAAAGTTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSGNTRLIMSLINQLILKCHCFSVIDTLMLIKEAWSYNLTIGCTSNELVQDQLSLFDVMSSELMNHKLPYM
IGQENYVEELRSESLVSLYREYILLRLSNYKPQLFTVNHVEFSYIRGSRDKNSWFALPDFRLRDRGGRSVWLKILGITKSLLTYFALNRKNENYSLLFKR
RKCDSDIPSILRISDDMDTFLIHLLEENSSHEFEVLGLQLCSFYGTLQDFTKS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU56255
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Please can you make constructs to express a fragment of S.cerevisiae Tel[phone]) in E. coli. Please then pass this to the protein expression and antibody team. We would like to make affinity purified polyclonal antibodies for this protein in SHEEP
• Low Temp: No