cDNA Clone CK1α

• Synonyms: casein kinase 1 isoform alpha
• Plasmid: pcDNA5-FRT/TO-mCherry-CK1α N141A
• Parent Plasmid: pcDNA5-FRT/TO-mCherry
• Expressed: mcherry-CK1α N141A
• Genbank: P48729.2
• Tag: mCherry
• Species: Human

Sequence of Insert

GGATCCATGGCGAGTAGCAGCGGCTCCAAGGCTGAATTCATTGTCGGAGG
GAAATATAAACTGGTACGGAAGATCGGGTCTGGCTCCTTCGGGGACATCT
ATTTGGCGATCAACATCACCAACGGCGAGGAAGTGGCAGTGAAGCTAGAA
TCTCAGAAGGCCAGGCATCCCCAGTTGCTGTACGAGAGCAAGCTCTATAA
GATTCTTCAAGGTGGGGTTGGCATCCCCCACATACGGTGGTATGGTCAGG
AAAAAGACTACAATGTACTAGTCATGGATCTTCTGGGACCTAGCCTCGAA
GACCTCTTCAATTTCTGTTCAAGAAGGTTCACAATGAAAACTGTACTTAT
GTTAGCTGACCAGATGATCAGTAGAATTGAATATGTGCATACAAAGAATT
TTATACACAGAGACATTAAACCAGATgcCTTCCTAATGGGTATTGGGCGT
CACTGTAATAAGTTATTCCTTATTGATTTTGGTTTGGCCAAAAAGTACAG
AGACAACAGGACAAGGCAACACATACCATACAGAGAAGATAAAAACCTCA
CTGGCACTGCCCGATATGCTAGCATCAATGCACATCTTGGTATTGAGCAG
AGTCGCCGAGATGACATGGAATCATTAGGATATGTTTTGATGTATTTTAA
TAGAACCAGCCTGCCATGGCAAGGGCTAAAGGCTGCAACAAAGAAACAAA
AATATGAAAAGATTAGTGAAAAGAAGATGTCCACGCCTGTTGAAGTTTTA
TGTAAGGGGTTTCCTGCAGAATTTGCGATGTACTTAAACTATTGTCGTGG
GCTACGCTTTGAGGAAGCCCCAGATTACATGTATCTGAGGCAGCTATTCC
GCATTCTTTTCAGGACCCTGAACCATCAATATGACTACACATTTGATTGG
ACAATGTTAAAGCAGAAAGCAGCACAGCAGGCAGCCTCTTCCAGTGGGCA
GGGTCAGCAGGCCCAAACCCCCACAGGCAAGCAAACTGACAAAACCAAGA
GTAACATGAAAGGTTTCTAAGCGGCCGC

Amino Acid Sequence

MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAK
LKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWER
VMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEA
SSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNV
NIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYKSGLGSMASSSGSKA
EFIVGGKYKLVRKIGSGSFGDIYLAINITNGEEVAVKLESQKARHPQLLY
ESKLYKILQGGVGIPHIRWYGQEKDYNVLVMDLLGPSLEDLFNFCSRRFT
MKTVLMLADQMISRIEYVHTKNFIHRDIKPDAFLMGIGRHCNKLFLIDFG
LAKKYRDNRTRQHIPYREDKNLTGTARYASINAHLGIEQSRRDDMESLGY
VLMYFNRTSLPWQGLKAATKKQKYEKISEKKMSTPVEVLCKGFPAEFAMY
LNYCRGLRFEEAPDYMYLRQLFRILFRTLNHQYDYTFDWTMLKQKAAQQA
ASSSGQGQQAQTPTGKQTDKTKSNMKGF*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU28839
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental 31558
• Low Temp: No