cDNA Clone FAM83H

• Plasmid: pGEX6P-1-FAM83H K229A
• Parent Plasmid: pGEX6P-1
• Expressed: GST-FAM83H K229A
• Genbank: NM_198488.3
• Tag: GST
• Species: Human

Sequence of Insert

ATGGCCCGTCGCTCTCAGAGCTCCTCGCAGGGGGACAACCCACTGGCACC
CGGGTACCTGCCGCCTCACTACAAAGAGTACTACCGCCTGGCGGTGGATG
CACTGGCCGAGGGTGGCTCGGAGGCCTACAGCCGCTTCCTCGCTACCGAG
GGGGCACCAGACTTCCTGTGCCCTGAAGAGCTGGAACATGTGAGCCGACA
CCTTCGGCCTCCGCAGTATGTTACCCGAGAGCCACCTGAAGGCAGCCTTC
TCGACGTGGACATGGATGGCTCCTCGGGTACATACTGGCCAGTGAACTCA
GACCAGGCCGTGCCTGAGCTTGATTTGGGCTGGCCTCTGACCTTCGGCTT
CCAGGGCACCGAGGTGACCACCTTGGTGCAGCCACCGCCCCCCGACAGCC
CCAGTATCAAGGATGAGGCCCGCAGGATGATCCGTTCCGCCCAGCAGGTG
GTGGCCGTGGTGATGGACATGTTCACTGATGTGGACCTGCTCAGCGAAGT
GCTGGAGGCCGCGGCCCGTCGGGTCCCAGTCTACATCCTGCTGGATGAGA
TGAACGCGCAGCACTTCCTGGACATGGCCGACAAGTGCCGTGTCAACCTG
CACCACGTGGATTTCCTGCGCGTACGGACTGTGGCGGGCCCCACCTACTA
CTGCCGCACTGGGAAGTCCTTCAAGGGCCACGTCgcGGAGAAGTTCCTGC
TGGTGGACTGTGCCGTGGTGATGAGTGGGAGCTACAGCTTCATGTGGTCC
TTTGAGAAGATCCACCGCAGCCTGGCGCACGTGTTCCAAGGAGAGCTGGT
CTCCAGCTTCGACGAGGAGTTCCGCATCCTCTTCGCGCAGTCCGAGCCGC
TTGTGCCCTCGGCCGCGGCCCTGGCCCGCATGGACGCCTATGCCCTGGCT
CCGTATGCCGGGGCCGGGCCTCTCGTGGGCGTCCCTGGGGTCGGGGCGCC
AACCCCCTTCTCCTTCCCTAAACGAGCGCACCTCCTGTTCCCGCCACCCC
GGGAAGAGGGCCTGGGCTTCCCCTCCTTCCTCGACCCGGACCGCCACTTC
CTGTCGGCCTTCCGCCGGGAGGAGCCGCCGCGGATGCCGGGGGGCGCGCT
GGAACCGCACGCGGGGCTGCGGCCACTCTCGCGGCGCCTGGAGGCCGAGG
CCGGGCCGGCTGGGGAGCTCGCGGGCGCGCGGGGCTTCTTCCAGGCGCGG
CACCTGGAGATGGACGCCTTCAAGCGGCACAGCTTCGCGACCGAGGGCGC
GGGCGCCGTGGAGAACTTCGCGGCTGCGCGGCAGGTGTCGCGGCAGACGT
TCCTCAGCCACGGCGACGACTTCCGCTTCCAGACCAGCCACTTCCACCGT
GACCAGCTCTACCAGCAGCAGTACCAGTGGGACCCGCAGCTCACGCCGGC
GCGCCCGCAAGGCCTGTTCGAGAAGCTTCGCGGGGGCCGCGCGGGTTTCG
CGGACCCGGATGACTTCACCCTGGGCGCCGGGCCCCGCTTCCCGGAGCTC
GGACCCGACGGGCACCAGCGGCTGGACTACGTGCCGTCCAGCGCGTCCCG
CGAGGTGCGCCACGGCTCGGACCCCGCCTTCGCGCCCGGACCCCGCGGCC
TGGAGCCCAGCGGAGCCCCGCGCCCCAACCTGACCCAGCGCTTCCCATGC
CAGGCCGCGGCGAGGCCGGGCCCAGACCCCGCTCCCGAGGCGGAGCCGGA
GCGCAGGGGCGGGCCCGAGGGGCGGGCAGGGCTGCGGCGCTGGCGTTTGG
CCTCCTACTTGAGCGGCTGCCACGGCGAGGATGGGGGCGACGACGGCCTA
CCGGCGCCCATGGAAGCGGAGGCTTACGAAGACGACGTGCTGGCTCCCGG
GGGCCGGGCACCTGCCGGCGACCTGCTCCCCTCGGCCTTCCGCGTCCCAG
CAGCCTTCCCCACCAAGGTCCCGGTGCCAGGCCCGGGCAGCGGCGGCAAC
GGCCCAGAGCGCGAGGGCCCGGAGGAGCCTGGCCTGGCCAAGCAGGACTC
ATTCCGCTCGCGCCTGAACCCCCTGGTCCAGCGCAGCTCCAGGCTGCGCT
CCTCGCTCATCTTCAGCACGTCACAGGCCGAGGGCGCGGCCGGGGCTGCG
GCGGCCACTGAGAAGGTGCAGCTGCTGCACAAGGAGCAGACGGTCAGCGA
GACGCTGGGGCCCGGCGGAGAGGCCGTGCGCTCCGCGGCTTCCACCAAGG
TGGCGGAGCTGCTGGAGAAGTACAAGGGCCCAGCCCGTGATCCCGGCGGC
GGCGCGGGCGCCATCACCGTTGCCAGCCACAGCAAGGCCGTCGTGTCCCA
GGCGTGGCGGGAAGAGGTGGCGGCCCCAGGTGCCGTGGGGGGCGAGCGCC
GCAGCCTCGAGAGCTGCCTGCTGGACCTGCGCGACTCCTTTGCACAGCAG
CTGCACCAGGAGGCGGAGCGGCAGCCGGGAGCCGCGTCGCTCACCGCGGC
GCAGCTGCTCGACACACTGGGCCGGAGCGGCTCCGACCGCCTGCCTTCCC
GCTTCCTCTCTGCCCAGAGCCACTCAACGTCCCCGCAAGGGCTGGACAGC
CCTCTGCCGCTGGAAGGGTCCGGAGCGCACCAGGTGCTCCATAATGAGTC
AAAAGGGAGCCCCACCTCGGCTTACCCTGAGCGGAAGGGGAGCCCCACGC
CTGGGTTTTCCACTCGAAGAGGAAGTCCAACTACAGGATTTATCGAGCAG
AAGGGGAGCCCCACCTCAGCCTACCCCGAGCGCAGGGGTAGTCCGGTGCC
CCCCGTGCCGGAGCGCAGGAGCAGTCCGGTGCCCCCCGTGCCGGAGCGCA
GGGGCAGCCTCACCCTTACCATCTCCGGGGAGTCCCCGAAGGCCGGGCCC
GCGGAGGAGGGGCCGAGCGGCCCCATGGAAGTCCTGCGCAAAGGCTCCTT
GCGTCTTAGGCAGCTGCTGAGCCCCAAGGGCGAGCGGCGCATGGAGGATG
AGGGTGGCTTCCCAGTGCCGCAGGAGAACGGCCAACCCGAGAGCCCGCGG
CGTCTGTCACTGGGCCAGGGTGACAGCACGGAGGCTGCCACAGAAGAGCG
GGGTCCGCGGGCGCGCCTGTCCTCAGCCACGGCCAACGCCTTGTACAGCA
GCAACCTTCGGGATGACACGAAGGCCATTCTGGAGCAGATCAGTGCCCAC
GGCCAGAAGCACCGTGCGGTCCCTGCCCCGAGCCCCGGCCCGACCCACAA
CAGCCCCGAGCTAGGCCGTCCACCGGCTGCTGGCGTCCTGGCCCCAGATA
TGTCCGACAAGGACAAGTGTTCAGCCATCTTCCGCTCGGACAGCTTGGGG
ACCCAGGGCCGGCTGAGCCGCACGCTGCCAGCCAGCGCGGAGGAGCGCGA
TCGGCTGCTGCGCCGCATGGAGAGCATGCGCAAGGAGAAGCGCGTGTACA
GCCGCTTCGAGGTCTTCTGCAAGAAAGAGGAGGCCAGCAGCCCTGGGGCA
GGGGAAGGCCCCGCGGAGGAGGGCACCAGGGACAGCAAGGTGGGCAAGTT
CGTGCCCAAGATCCTGGGCACGTTCAAAAGCAAGAAGtgagtcgac

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLMARRSQSSSQGDNPLAPGYLP
PHYKEYYRLAVDALAEGGSEAYSRFLATEGAPDFLCPEELEHVSRHLRPP
QYVTREPPEGSLLDVDMDGSSGTYWPVNSDQAVPELDLGWPLTFGFQGTE
VTTLVQPPPPDSPSIKDEARRMIRSAQQVVAVVMDMFTDVDLLSEVLEAA
ARRVPVYILLDEMNAQHFLDMADKCRVNLHHVDFLRVRTVAGPTYYCRTG
KSFKGHVAEKFLLVDCAVVMSGSYSFMWSFEKIHRSLAHVFQGELVSSFD
EEFRILFAQSEPLVPSAAALARMDAYALAPYAGAGPLVGVPGVGAPTPFS
FPKRAHLLFPPPREEGLGFPSFLDPDRHFLSAFRREEPPRMPGGALEPHA
GLRPLSRRLEAEAGPAGELAGARGFFQARHLEMDAFKRHSFATEGAGAVE
NFAAARQVSRQTFLSHGDDFRFQTSHFHRDQLYQQQYQWDPQLTPARPQG
LFEKLRGGRAGFADPDDFTLGAGPRFPELGPDGHQRLDYVPSSASREVRH
GSDPAFAPGPRGLEPSGAPRPNLTQRFPCQAAARPGPDPAPEAEPERRGG
PEGRAGLRRWRLASYLSGCHGEDGGDDGLPAPMEAEAYEDDVLAPGGRAP
AGDLLPSAFRVPAAFPTKVPVPGPGSGGNGPEREGPEEPGLAKQDSFRSR
LNPLVQRSSRLRSSLIFSTSQAEGAAGAAAATEKVQLLHKEQTVSETLGP
GGEAVRSAASTKVAELLEKYKGPARDPGGGAGAITVASHSKAVVSQAWRE
EVAAPGAVGGERRSLESCLLDLRDSFAQQLHQEAERQPGAASLTAAQLLD
TLGRSGSDRLPSRFLSAQSHSTSPQGLDSPLPLEGSGAHQVLHNESKGSP
TSAYPERKGSPTPGFSTRRGSPTTGFIEQKGSPTSAYPERRGSPVPPVPE
RRSSPVPPVPERRGSLTLTISGESPKAGPAEEGPSGPMEVLRKGSLRLRQ
LLSPKGERRMEDEGGFPVPQENGQPESPRRLSLGQGDSTEAATEERGPRA
RLSSATANALYSSNLRDDTKAILEQISAHGQKHRAVPAPSPGPTHNSPEL
GRPPAAGVLAPDMSDKDKCSAIFRSDSLGTQGRLSRTLPASAEERDRLLR
RMESMRKEKRVYSRFEVFCKKEEASSPGAGEGPAEEGTRDSKVGKFVPKI
LGTFKSKK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU28851
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Silent changes at Pro375 and Ala425 compared to NCBI ref. Can be excised Bam-Sal or Bam-Not (not1 site just downstream of Sal1)
• Low Temp: No