cDNA Clone HA-APEX2-FIP200 (ATG17)

• Plasmid: pBABE puro HA-APEX2-FIP200 (ATG17)
• Parent Plasmid: pBABE puro HA-APEX2
• Expressed: HA-APEX2-FIP200 (ATG17)
• Tag: HA
• Tag: APEX2

Sequence of Insert

GCGGCCGCGATGAAGTTATATGTATTTCTGGTTAACACTGGAACTACTCTAACATTTGACACTGAACTTACAGTGCAAACTGTGGCAGACCTTAAGCATGCCATTCAAAGCAAATACAAGATTGCTATTCAACACCAGGTGCTGGTGGTCAATGGAGGAGAATGCATGGCTGCAGATCGAAGAGTGTGTACCTACAGTGCTGGGACGGATACAAATCCAATTTTTCTTTTTAACAAAGAAATGATCTTATGTGATCGTCCACCTGCTATTCCTAAAACTACCTTTTCGACAGAAAATGACATGGAAATAAAAGTTGAAGAATCTCTTATGATGCCTGCAGTTTTTCATACTGTTGCTTCAAGGACACAGCTTGCATTGGAAATGTATGAAGTTGCCAAGAAACTTTGTTCTTTTTGTGAAGGTCTTGTACATGATGAACATCTTCAACACCAAGGCTGGGCTGCAATCATGGCCAACCTGGAGGACTGTTCAAATTCATACCAAAAGCTACTTTTCAAGTTTGAAAGTATTTATTCAAATTATCTGCAGTCCATAGAAGACATCAAGTTAAAACTTACTCATTTAGGAACTGCAGTTTCAGTAATGGCCAAGATTCCACTGTTGGAGTGCCTAACCAGACATAGTTACAGAGAATGTTTGGGAAGACTGGATTCTTTACCTGAACATGAAGACTCAGAAAAAGCTGAGACGAAAAGATCCACTGAACTGGTGCTCTCTCCTGATATGCCTAGAACAACTAACGAATCTTTGTTAACCTCATTTCCCAAGTCAGTGGAACATGTGTCCCCAGATACCGCAGATGCTGAAAGTGGCAAAGAAATTAGGGAATCTTGTCAAAGTACTGTTCATCAGCAAGATGAAACTACGATTGACACTAAAGATGGTGATCTGCCCTTTTTTAATGTCTCTTTGTTAGACTGGATAAATGTTCAAGATAGACCTAATGATGTGGAATCTTTGGTCAGGAAGTGCTTTGATTCTATGAGCAGGCTTGATCCAAGGATTATTCGACCATTTATAGCAGAATGCCGTCAAACTATTGCCAAACTTGATAATCAGAATATGAAAGCCATTAAAGGACTTGAAGATCGGCTCTACGCCCTGGACCAGATGATTGCTAGCTGTGGCCGACTGGTGAATGAACAGAAAGAGCTTGCTCAGGGATTTTTAGCTAATCAGAAGAGAGCTGAAAACTTAAAGGATGCATCTGTATTACCTGATTTATGCCTGAGTCACGCAAATCAGTTGATGATTATGTTGCAAAATCATAGAAAACTGTTAGATATTAAGCAGAAGTGTACCACTGCCAAACAAGAACTAGCAAATAACCTACATGTCAGACTGAAGTGGTGTTGCTTTGTAATGCTTCATGCTGATCAAGATGGAGAGAAGTTACAAGCTTTGCTCCGCCTCGTAATAGAGCTGTTAGAAAGAGTCAAAATTGTTGAAGCTCTTAGTACAGTTCCTCAGATGTACTGCTTAGCTGTTGTTGAGGTTGTAAGAAGAAAAATGTTCATAAAACACTACAGGGAGTGGGCTGGTGCTTTAGTCAAAGATGGAAAGAGATTATATGAAGCAGAAAAATCAAAAAGGGAATCCTTTGGGAAATTATTTAGGAAGTCTTTTTTAAGAAATCGTCTGTTTAGGGGACTGGACTCCTGGCCCCCTTCCTTTTGTACTCAAAAGCCTCGAAAGTTTGACTGTGAACTTCCAGATATTTCATTAAAAGATTTACAGTTTCTGCAATCATTTTGTCCTTCGGAAGTTCAGCCATTCCTCAGGGTTCCCTTACTTTGTGACTTTGAACCTCTACACCAGCATGTACTTGCTCTACATAATTTGGTAAAAGCAGCACAAAGTTTGGATGAAATGTCACAGACCATTACAGATCTACTGAGTGAACAAAAGGCATCTGTGAGTCAGACATCCCCACAGTCTGCTTCTTCACCAAGGATGGAAAGTACAGCAGGAATTACAACTACTACCTCACCGAGAACTCCTCCACCACTGACTGTTCAGGATCCCTTATGTCCTGCAGTTTGTCCCTTAGAAGAATTATCTCCAGATAGTATTGATGCACATACGTTTGATTTTGAAACTATTCCCCATCCAAACATAGAACAGACTATTCACCAAGTTTCTTTAGACTTGGATTCATTAGCAGAAAGTCCTGAATCAGATTTTATGTCTGCTGTGAATGAGTTTGTAATAGAAGAAAATTTGTCGTCTCCTAATCCTATAAGTGATCCACAAAGCCCAGAAATGATGGTGGAATCACTTTATTCATCAGTTATCAATGCGATAGACAGTAGACGAATGCAGGATACAAATGTATGTGGTAAGGAGGATTTTGGAGATCATACTTCTCTGAATGTCCAGTTGGAAAGATGTAGAGTTGTTGCCCAAGACTCTCACTTCAGTATACAAACCATTAAGGAAGACCTTTGCCACTTTAGAACATTTGTACAAAAAGAACAGTGTGACTTCTCAAATTCATTAAAATGTACAGCAGTAGAAATAAGAAACATTATTGAAAAAGTAAAATGTTCTCTGGAAATAACACTAAAAGAAAAACATCAAAAAGAACTACTGTCTTTAAAAAATGAATATGAAGGTAAACTTGACGGACTAATAAAGGAAACTGAAGAGAATGAAAACAAAATTAAAAAATTGAAGGGAGAGTTAGTATGCCTTGAGGAGGTTTTACAAAATAAAGATAATGAATTTGCTTTGGTTAAACATGAAAAAGAAGCTGTAATCTGCCTGCAGAATGAAAAGGATCAGAAGTTGTTAGAGATGGAAAATATAATGCACTCTCAAAATTGTGAAATTAAAGAACTGAAGCAGTCACGAGAAATAGTGTTAGAAGACTTAAAAAAGCTCCATGTTGAAAATGATGAGAAGTTACAGTTATTGAGGGCAGAACTTCAGTCCTTGGAGCAAAGTCATCTAAAGGAATTAGAGGACACACTTCAGGTTAGGCACATACAAGAGTTTGAGAAGGTTATGACAGACCACAGAGTTTCTTTGGAGGAATTAAAAAAGGAAAACCAACAAATAATTAATCAAATACAAGAATCTCATGCTGAAATTATCCAGGAAAAAGAAAAACAGTTACAGGAATTAAAACTCAAGGTTTCTGATTTGTCAGACACGAGATGCAAGTTAGAGGTTGAACTTGCGTTGAAGGAAGCAGAAACTGATGAAATAAAAATTTTGCTGGAAGAAAGCAGAGCCCAGCAGAAGGAGACCTTGAAATCTCTTCTTGAACAAGAGACAGAAAATTTGAGAACAGAAATTAGTAAACTCAACCAAAAGATTCAGGATAATAATGAAAATTATCAGGTGGGCTTAGCAGAGCTAAGAACTTTAATGACAATTGAAAAAGATCAGTGTATTTCCGAGTTAATTAGTAGACATGAAGAAGAATCTAATATACTTAAAGCTGAATTAAACAAAGTAACATCTTTGCATAACCAAGCATTTGAAATAGAAAAAAACCTAAAAGAACAAATAATTGAACTGCAGAGTAAATTGGATTCAGAATTGAGTGCTCTTGAAAGACAAAAAGATGAAAAAATTACCCAACAAGAAGAGAAATACGAAGCTATTATCCAGAACCTTGAGAAAGACAGACAAAAATTGGTCAGCAGCCAGGAGCAAGACAGAGAACAGTTAATTCAGAAGCTTAATTGTGAAAAAGATGAAGCTATTCAGACTGCCCTAAAAGAATTTAAATTGGAGAGAGAAGTTGTTGAGAAAGAGTTATTAGAAAAAGTTAAACATCTTGAGAATCAAATAGCAAAAAGTCCTGCCATTGACTCTACCAGAGGAGATTCTTCAAGCTTAGTTGCTGAACTTCAAGAAAAGCTTCAGGAAGAAAAAGCTAAGTTTCTAGAACAACTTGAAGAGCAAGAAAAAAGAAAGAATGAAGAAATGCAAAATGTTCGAACATCTTTGATTGCGGAACAACAGACCAATTTTAACACTGTTTTAACAAGAGAGAAAATGAGAAAAGAAAACATAATAAATGATCTTAGTGATAAGTTGAAAAGTACAATGCAGCAACAAGAACGGGATAAAGATTTGATAGAGTCACTTTCTGAAGATCGAGCTCGTTTGCTTGAGGAAAAGAAAAAGCTTGAAGAAGAAGTCAGTAAGTTGCGCAGTAGCAGTTTTGTTCCTTCACCATATGTAGCTACAGCCCCAGAACTTTATGGAGCTTGTGCACCTGAACTCCCAGGTGAATCAGATAGATCCGCTGTGGAAACAGCAGATGAAGGAAGAGTGGATTCAGCAATGGAGACAAGCATGATGTCTGTACAAGAAAATATTCATATGTTGTCTGAAGAAAAACAGCGGATAATGCTGTTAGAACGAACATTGCAATTGAAAGAAGAAGAAAATAAACGGTTAAATCAAAGACTGATGTCTCAGAGCATGTCTTCAGTATCTTCAAGGCATTCTGAAAAGATAGCTATTAGAGATTTTCAGGTGGGAGATTTGGTACTCATCATCCTAGACGAACGCCATGACAATTATGTGTTATTTACTGTTAGTCCTACTTTATATTTTCTACATTCAGAGTCTCTACCTGCCCTGGATCTCAAACCAGGTGAGGGTGCTTCAGGTGCATCTAGAAGACCCTGGGTACTTGGAAAAGTAATGGAAAAAGAATACTGTCAAGCCAAAAAGGCACAAAACAGATTTAAAGTTCCTTTGGGGACAAAGTTTTACAGAGTGAAAGCCGTATCATGGAATAAGAAAGTATAACGCGGCCGC

Amino Acid Sequence

GSMYPYDVPDYAGGGGGGKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGTFDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAVEVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHTIGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDKYAADEDAFFADYAEAHQKLSELGFADAEFEVDSLESAAAMKLYVFLVNTGTTLTFDTELTVQTVADLKHAIQSKYKIAIQHQVLVVNGGECMAADRRVCTYSAGTDTNPIFLFNKEMILCDRPPAIPKTTFSTENDMEIKVEESLMMPAVFHTVASRTQLALEMYEVAKKLCSFCEGLVHDEHLQHQGWAAIMANLEDCSNSYQKLLFKFESIYSNYLQSIEDIKLKLTHLGTAVSVMAKIPLLECLTRHSYRECLGRLDSLPEHEDSEKAETKRSTELVLSPDMPRTTNESLLTSFPKSVEHVSPDTADAESGKEIRESCQSTVHQQDETTIDTKDGDLPFFNVSLLDWINVQDRPNDVESLVRKCFDSMSRLDPRIIRPFIAECRQTIAKLDNQNMKAIKGLEDRLYALDQMIASCGRLVNEQKELAQGFLANQKRAENLKDASVLPDLCLSHANQLMIMLQNHRKLLDIKQKCTTAKQELANNLHVRLKWCCFVMLHADQDGEKLQALLRLVIELLERVKIVEALSTVPQMYCLAVVEVVRRKMFIKHYREWAGALVKDGKRLYEAEKSKRESFGKLFRKSFLRNRLFRGLDSWPPSFCTQKPRKFDCELPDISLKDLQFLQSFCPSEVQPFLRVPLLCDFEPLHQHVLALHNLVKAAQSLDEMSQTITDLLSEQKASVSQTSPQSASSPRMESTAGITTTTSPRTPPPLTVQDPLCPAVCPLEELSPDSIDAHTFDFETIPHPNIEQTIHQVSLDLDSLAESPESDFMSAVNEFVIEENLSSPNPISDPQSPEMMVESLYSSVINAIDSRRMQDTNVCGKEDFGDHTSLNVQLERCRVVAQDSHFSIQTIKEDLCHFRTFVQKEQCDFSNSLKCTAVEIRNIIEKVKCSLEITLKEKHQKELLSLKNEYEGKLDGLIKETEENENKIKKLKGELVCLEEVLQNKDNEFALVKHEKEAVICLQNEKDQKLLEMENIMHSQNCEIKELKQSREIVLEDLKKLHVENDEKLQLLRAELQSLEQSHLKELEDTLQVRHIQEFEKVMTDHRVSLEELKKENQQIINQIQESHAEIIQEKEKQLQELKLKVSDLSDTRCKLEVELALKEAETDEIKILLEESRAQQKETLKSLLEQETENLRTEISKLNQKIQDNNENYQVGLAELRTLMTIEKDQCISELISRHEEESNILKAELNKVTSLHNQAFEIEKNLKEQIIELQSKLDSELSALERQKDEKITQQEEKYEAIIQNLEKDRQKLVSSQEQDREQLIQKLNCEKDEAIQTALKEFKLEREVVEKELLEKVKHLENQIAKSPAIDSTRGDSSSLVAELQEKLQEEKAKFLEQLEEQEKRKNEEMQNVRTSLIAEQQTNFNTVLTREKMRKENIINDLSDKLKSTMQQQERDKDLIESLSEDRARLLEEKKKLEEEVSKLRSSSFVPSPYVATAPELYGACAPELPGESDRSAVETADEGRVDSAMETSMMSVQENIHMLSEEKQRIMLLERTLQLKEEENKRLNQRLMSQSMSSVSSRHSEKIAIRDFQVGDLVLIILDERHDNYVLFTVSPTLYFLHSESLPALDLKPGEGASGASRRPWVLGKVMEKEYCQAKKAQNRFKVPLGTKFYRVKAVSWNKKV*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU55962
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Please introduce FIP200 in DU55714. Instead of Flag tag, HA tag please. Thank you!
• Low Temp: No