cDNA Clone FAM83D

• Plasmid: pcDNA5-FRT/TO-GFP-FAM83D T439A/Q440A/T441A
• Parent Plasmid: pcDNA5-FRT/TO-GFP
• Expressed: GFP-FAM83D T439A/Q440A/T441A
• Genbank: NM_030919.2
• Tag: GFP
• Species: Human

Sequence of Insert

ggatccatggcgcgggcctgtttgattcaaaggttgcctataaagcggga
ctgcacgccggtttttgtccgagggctgtcgagtccgagcgccgccatgg
ctctgctgtccgagggcctggacgaggtgcccgccgcctgcctgtcgccg
tgcgggccgcccaacccgaccgagctgttcagcgagtcacggcgcctggc
tctggaggagctggtggcgggcggccccgaagccttcgcggccttcctgc
gacgcgagcgcctggctcgtttcctgaaccccgatgaggtgcacgccatt
ctgcgcgcggcggagaggccgggagaggagggcgcggcggcggcggcggc
ggccgaggactcgttcggctcctcgcacgactgctcttcgggcacctact
tccccgagcagtcggacctggagccaccgctgttggagcttggctggccc
gccttctaccagggcgcctaccgcggcgccacgcgtgtcgagacgcactt
ccagccccgcggcgctggcgaaggtggcccctacggctgcaaggacgctc
tgcgccagcagctccgctcggcgcgagaggtgattgcagtggtcatggac
gtgttcacagacatcgacatcttcagagacctgcaagaaatatgcaggaa
acagggagttgctgtgtatatccttctggaccaggctctcctctctcaat
ttctggatatgtgcatggatctgaaagttcatcctgaacaggaaaagtta
atgacagttcggactatcacaggaaatatctactatgcaaggtcaggaac
taagattattgggaaggttcacgaaaagttcacgttgattgatggcatcc
gcgtggcaacaggctcctacagttttacatggacggatggcaaattaaac
agcagtaacttggtaattctgtctggccaagtggttgaacactttgatct
ggaattccgaatcctgtatgcccagtccaagcccatcagccccaaactcc
tgtctcacttccagagcagcaacaagtttgatcacctcaccaaccgaaaa
ccacagtccaaggagctcaccctgggcaacctgctgcggatgcggctggc
taggctgtcaagtactcccaggaaggcggacctggacccagagatgcccg
cagagggcaaggcagagcgcaagccccatgactgtgagtcctctactgtt
agtgaggaagactacttcagcagccacagggacgagctccagagcagaaa
ggccattgacgctgccactcaaacagagccaggagaggagatgccagggc
tgagtgtgagtgagGTGGGAGCAGCTGCGAGCatcaccacagcatgtgct
ggtacccagactgcagtcatcaccaggatagcaagctctcaaaccacgat
ttggtccagatcgaccactactcagactgacatggatgagaacattctct
ttcctcgaggaactcaatctacagaagggtcaccagtctcaaaaatgtct
gtatcgagatcttccagtttgaagtcttcctcctctgtgtcttcccaagg
ctctgtggcaagctccactggttctcccgcttccatcagaaccactgact
tccacaatcctggctatcccaagtacctgggcaccccccacctggaactg
tacttgagtgactcacttagaaacttgaacaaagagcggcaattccactt
cgctggtatcaggtcccggctcaaccacatgctggctatgctgtcaagga
gaacactctttactgaaaaccaccttggccttcattctggcaatttcagc
agagttaatttgcttgctgttagagatgtagcactttatccttcctatca
gtaaGTCGACTCGAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMARACL
IQRLPIKRDCTPVFVRGLSSPSAAMALLSEGLDEVPAACLSPCGPPNPTE
LFSESRRLALEELVAGGPEAFAAFLRRERLARFLNPDEVHAILRAAERPG
EEGAAAAAAAEDSFGSSHDCSSGTYFPEQSDLEPPLLELGWPAFYQGAYR
GATRVETHFQPRGAGEGGPYGCKDALRQQLRSAREVIAVVMDVFTDIDIF
RDLQEICRKQGVAVYILLDQALLSQFLDMCMDLKVHPEQEKLMTVRTITG
NIYYARSGTKIIGKVHEKFTLIDGIRVATGSYSFTWTDGKLNSSNLVILS
GQVVEHFDLEFRILYAQSKPISPKLLSHFQSSNKFDHLTNRKPQSKELTL
GNLLRMRLARLSSTPRKADLDPEMPAEGKAERKPHDCESSTVSEEDYFSS
HRDELQSRKAIDAATQTEPGEEMPGLSVSEVGAAASITTACAGTQTAVIT
RIASSQTTIWSRSTTTQTDMDENILFPRGTQSTEGSPVSKMSVSRSSSLK
SSSSVSSQGSVASSTGSPASIRTTDFHNPGYPKYLGTPHLELYLSDSLRN
LNKERQFHFAGIRSRLNHMLAMLSRRTLFTENHLGLHSGNFSRVNLLAVR
DVALYPSYQ*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU28881
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Silent mutation at E316
• Low Temp: No