cDNA Clone HRAS

• Synonyms: C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV1, RASH1, p21ras
• Plasmid: pBABED P aHRAS-HALO
• Parent Plasmid: pBabeD puro
• Expressed: aHRAS-5xGLY-HALO nanobody
• Species: Human

Sequence of Insert

aHRAS
GGATCCGCCATGGGCAGCGTGAGCAGCGTGCCCACCAAGCTGGAGGTGGTGGCCGCCACCCCCACCAGCCTGCTGATCAGCTGGGACGCCCCCGCCGTGACCGTGGACTACTACGTGATCACCTACGGCGAGACCGGCGGCAACAGCCCCGTGCAGAAGTTCGAGGTGCCCGGCAGCAAGAGCACCGCCACCATCAGCGGCCTGAAGCCCGGCGTGGACTACACCATCACCGTGTACGCCTGGGGCTGGCACGGCCAGGTGTACTACTACATGGGCAGCCCCATCAGCATCAACTACAGGACCGGTGGAGGCGGAGGTgaattc

HALO
gaattcgccATGGCAGAAATCGGTACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGTCGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTCCTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGACCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGTCCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCACGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGGTATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGCCCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCAGAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGAGATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTTCCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATACATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGTTCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGTGGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGAGATCGCGCGCTGGCTGTCTACGCTAGAGATTTCCGGCtgagcggccgc

Amino Acid Sequence

MGSVSSVPTKLEVVAATPTSLLISWDAPAVTVDYYVITYGETGGNSPVQKFEVPGSKSTATISGLKPGVDYTITVYAWGWHGQVYYYMGSPISINYRTGG
GGGEFAMAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEA
LGLEEVVLVIHDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFIEGTLPMGVVRPLTEVEMDHYREPFL
NPVDREPLWRFPNELPIAGEPANIVALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIGSEIARWLSTLE
ISG*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pBABED P aHRAS-HALO145.14 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU57429
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Anti-HRAS nanobody for use in degron work.
• Low Temp: No