cDNA Clone FAM83H

• Plasmid: pcDNA5-FRT/TO-FLAG-FAM83H F350A
• Parent Plasmid: pcDNA5-FRT/TO-FLAG
• Expressed: FLAG-FAM83H F350A
• Genbank: NM_198488.3
• Tag: FLAG
• Species: Human

Sequence of Insert

ggatccATGGCCCGTCGCTCTCAGAGCTCCTCGCAGGGGGACAACCCACT
GGCACCCGGGTACCTGCCGCCTCACTACAAAGAGTACTACCGCCTGGCGG
TGGATGCACTGGCCGAGGGTGGCTCGGAGGCCTACAGCCGCTTCCTCGCT
ACCGAGGGGGCACCAGACTTCCTGTGCCCTGAAGAGCTGGAACATGTGAG
CCGACACCTTCGGCCTCCGCAGTATGTTACCCGAGAGCCACCTGAAGGCA
GCCTTCTCGACGTGGACATGGATGGCTCCTCGGGTACATACTGGCCAGTG
AACTCAGACCAGGCCGTGCCTGAGCTTGATTTGGGCTGGCCTCTGACCTT
CGGCTTCCAGGGCACCGAGGTGACCACCTTGGTGCAGCCACCGCCCCCCG
ACAGCCCCAGTATCAAGGATGAGGCCCGCAGGATGATCCGTTCCGCCCAG
CAGGTGGTGGCCGTGGTGATGGACATGTTCACTGATGTGGACCTGCTCAG
CGAAGTGCTGGAGGCCGCGGCCCGTCGGGTCCCAGTCTACATCCTGCTGG
ATGAGATGAACGCGCAGCACTTCCTGGACATGGCCGACAAGTGCCGTGTC
AACCTGCACCACGTGGATTTCCTGCGCGTACGGACTGTGGCGGGCCCCAC
CTACTACTGCCGCACTGGGAAGTCCTTCAAGGGCCACGTCAAGGAGAAGT
TCCTGCTGGTGGACTGTGCCGTGGTGATGAGTGGGAGCTACAGCTTCATG
TGGTCCTTTGAGAAGATCCACCGCAGCCTGGCGCACGTGTTCCAAGGAGA
GCTGGTCTCCAGCTTCGACGAGGAGTTCCGCATCCTCTTCGCGCAGTCCG
AGCCGCTTGTGCCCTCGGCCGCGGCCCTGGCCCGCATGGACGCCTATGCC
CTGGCTCCGTATGCCGGGGCCGGGCCTCTCGTGGGCGTCCCTGGGGTCGG
GGCGCCAACCCCCTTCTCCTTCCCTAAACGAGCGCACCTCCTGTTCCCGC
CACCCCGGGAAGAGGGCCTGGGCTTCCCCTCCTTCCTCGACCCGGACCGC
CACgcCCTGTCGGCCTTCCGCCGGGAGGAGCCGCCGCGGATGCCGGGGGG
CGCGCTGGAACCGCACGCGGGGCTGCGGCCACTCTCGCGGCGCCTGGAGG
CCGAGGCCGGGCCGGCTGGGGAGCTCGCGGGCGCGCGGGGCTTCTTCCAG
GCGCGGCACCTGGAGATGGACGCCTTCAAGCGGCACAGCTTCGCGACCGA
GGGCGCGGGCGCCGTGGAGAACTTCGCGGCTGCGCGGCAGGTGTCGCGGC
AGACGTTCCTCAGCCACGGCGACGACTTCCGCTTCCAGACCAGCCACTTC
CACCGTGACCAGCTCTACCAGCAGCAGTACCAGTGGGACCCGCAGCTCAC
GCCGGCGCGCCCGCAAGGCCTGTTCGAGAAGCTTCGCGGGGGCCGCGCGG
GTTTCGCGGACCCGGATGACTTCACCCTGGGCGCCGGGCCCCGCTTCCCG
GAGCTCGGACCCGACGGGCACCAGCGGCTGGACTACGTGCCGTCCAGCGC
GTCCCGCGAGGTGCGCCACGGCTCGGACCCCGCCTTCGCGCCCGGACCCC
GCGGCCTGGAGCCCAGCGGAGCCCCGCGCCCCAACCTGACCCAGCGCTTC
CCATGCCAGGCCGCGGCGAGGCCGGGCCCAGACCCCGCTCCCGAGGCGGA
GCCGGAGCGCAGGGGCGGGCCCGAGGGGCGGGCAGGGCTGCGGCGCTGGC
GTTTGGCCTCCTACTTGAGCGGCTGCCACGGCGAGGATGGGGGCGACGAC
GGCCTACCGGCGCCCATGGAAGCGGAGGCTTACGAAGACGACGTGCTGGC
TCCCGGGGGCCGGGCACCTGCCGGCGACCTGCTCCCCTCGGCCTTCCGCG
TCCCAGCAGCCTTCCCCACCAAGGTCCCGGTGCCAGGCCCGGGCAGCGGC
GGCAACGGCCCAGAGCGCGAGGGCCCGGAGGAGCCTGGCCTGGCCAAGCA
GGACTCATTCCGCTCGCGCCTGAACCCCCTGGTCCAGCGCAGCTCCAGGC
TGCGCTCCTCGCTCATCTTCAGCACGTCACAGGCCGAGGGCGCGGCCGGG
GCTGCGGCGGCCACTGAGAAGGTGCAGCTGCTGCACAAGGAGCAGACGGT
CAGCGAGACGCTGGGGCCCGGCGGAGAGGCCGTGCGCTCCGCGGCTTCCA
CCAAGGTGGCGGAGCTGCTGGAGAAGTACAAGGGCCCAGCCCGTGATCCC
GGCGGCGGCGCGGGCGCCATCACCGTTGCCAGCCACAGCAAGGCCGTCGT
GTCCCAGGCGTGGCGGGAAGAGGTGGCGGCCCCAGGTGCCGTGGGGGGCG
AGCGCCGCAGCCTCGAGAGCTGCCTGCTGGACCTGCGCGACTCCTTTGCA
CAGCAGCTGCACCAGGAGGCGGAGCGGCAGCCGGGAGCCGCGTCGCTCAC
CGCGGCGCAGCTGCTCGACACACTGGGCCGGAGCGGCTCCGACCGCCTGC
CTTCCCGCTTCCTCTCTGCCCAGAGCCACTCAACGTCCCCGCAAGGGCTG
GACAGCCCTCTGCCGCTGGAAGGGTCCGGAGCGCACCAGGTGCTCCATAA
TGAGTCAAAAGGGAGCCCCACCTCGGCTTACCCTGAGCGGAAGGGGAGCC
CCACGCCTGGGTTTTCCACTCGAAGAGGAAGTCCAACTACAGGATTTATC
GAGCAGAAGGGGAGCCCCACCTCAGCCTACCCCGAGCGCAGGGGTAGTCC
GGTGCCCCCCGTGCCGGAGCGCAGGAGCAGTCCGGTGCCCCCCGTGCCGG
AGCGCAGGGGCAGCCTCACCCTTACCATCTCCGGGGAGTCCCCGAAGGCC
GGGCCCGCGGAGGAGGGGCCGAGCGGCCCCATGGAAGTCCTGCGCAAAGG
CTCCTTGCGTCTTAGGCAGCTGCTGAGCCCCAAGGGCGAGCGGCGCATGG
AGGATGAGGGTGGCTTCCCAGTGCCGCAGGAGAACGGCCAACCCGAGAGC
CCGCGGCGTCTGTCACTGGGCCAGGGTGACAGCACGGAGGCTGCCACAGA
AGAGCGGGGTCCGCGGGCGCGCCTGTCCTCAGCCACGGCCAACGCCTTGT
ACAGCAGCAACCTTCGGGATGACACGAAGGCCATTCTGGAGCAGATCAGT
GCCCACGGCCAGAAGCACCGTGCGGTCCCTGCCCCGAGCCCCGGCCCGAC
CCACAACAGCCCCGAGCTAGGCCGTCCACCGGCTGCTGGCGTCCTGGCCC
CAGATATGTCCGACAAGGACAAGTGTTCAGCCATCTTCCGCTCGGACAGC
TTGGGGACCCAGGGCCGGCTGAGCCGCACGCTGCCAGCCAGCGCGGAGGA
GCGCGATCGGCTGCTGCGCCGCATGGAGAGCATGCGCAAGGAGAAGCGCG
TGTACAGCCGCTTCGAGGTCTTCTGCAAGAAAGAGGAGGCCAGCAGCCCT
GGGGCAGGGGAAGGCCCCGCGGAGGAGGGCACCAGGGACAGCAAGGTGGG
CAAGTTCGTGCCCAAGATCCTGGGCACGTTCAAAAGCAAGAAGtgagcgg
ccgc

Amino Acid Sequence

MDYKDDDDKGSMARRSQSSSQGDNPLAPGYLPPHYKEYYRLAVDALAEGG
SEAYSRFLATEGAPDFLCPEELEHVSRHLRPPQYVTREPPEGSLLDVDMD
GSSGTYWPVNSDQAVPELDLGWPLTFGFQGTEVTTLVQPPPPDSPSIKDE
ARRMIRSAQQVVAVVMDMFTDVDLLSEVLEAAARRVPVYILLDEMNAQHF
LDMADKCRVNLHHVDFLRVRTVAGPTYYCRTGKSFKGHVKEKFLLVDCAV
VMSGSYSFMWSFEKIHRSLAHVFQGELVSSFDEEFRILFAQSEPLVPSAA
ALARMDAYALAPYAGAGPLVGVPGVGAPTPFSFPKRAHLLFPPPREEGLG
FPSFLDPDRHALSAFRREEPPRMPGGALEPHAGLRPLSRRLEAEAGPAGE
LAGARGFFQARHLEMDAFKRHSFATEGAGAVENFAAARQVSRQTFLSHGD
DFRFQTSHFHRDQLYQQQYQWDPQLTPARPQGLFEKLRGGRAGFADPDDF
TLGAGPRFPELGPDGHQRLDYVPSSASREVRHGSDPAFAPGPRGLEPSGA
PRPNLTQRFPCQAAARPGPDPAPEAEPERRGGPEGRAGLRRWRLASYLSG
CHGEDGGDDGLPAPMEAEAYEDDVLAPGGRAPAGDLLPSAFRVPAAFPTK
VPVPGPGSGGNGPEREGPEEPGLAKQDSFRSRLNPLVQRSSRLRSSLIFS
TSQAEGAAGAAAATEKVQLLHKEQTVSETLGPGGEAVRSAASTKVAELLE
KYKGPARDPGGGAGAITVASHSKAVVSQAWREEVAAPGAVGGERRSLESC
LLDLRDSFAQQLHQEAERQPGAASLTAAQLLDTLGRSGSDRLPSRFLSAQ
SHSTSPQGLDSPLPLEGSGAHQVLHNESKGSPTSAYPERKGSPTPGFSTR
RGSPTTGFIEQKGSPTSAYPERRGSPVPPVPERRSSPVPPVPERRGSLTL
TISGESPKAGPAEEGPSGPMEVLRKGSLRLRQLLSPKGERRMEDEGGFPV
PQENGQPESPRRLSLGQGDSTEAATEERGPRARLSSATANALYSSNLRDD
TKAILEQISAHGQKHRAVPAPSPGPTHNSPELGRPPAAGVLAPDMSDKDK
CSAIFRSDSLGTQGRLSRTLPASAEERDRLLRRMESMRKEKRVYSRFEVF
CKKEEASSPGAGEGPAEEGTRDSKVGKFVPKILGTFKSKK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU28889
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Silent changes at Pro375 and Ala425 compared to NCBI ref.
• Low Temp: No