cDNA Clone ULK1 (rat)

• Plasmid: pBabeD puro (rat) ULK1 (shRNA res 601-630)
• Parent Plasmid: pBabeD puro
• Expressed: ULK1 shRNA resistant 601-630
• Genbank: NM_001108341

Sequence of Insert

GGATCCATGGAGCCGGGCCGCGGCGGTGTCGAGACCGTGGGCAAGTTCGAGTTCTCTCGCAAAGACCTGATTGGACACGGCGCCTTCGCGGTGGTCTTCAAGGGTCGACACCGCGAGAAGCACGACCTGGAGGTGGCCGTCAAATGCATTAACAAGAAGAACCTTGCCAAGTCCCAGACACTGCTAGGAAAGGAAATCAAAATCCTGAAGGAACTAAAGCACGAAAACATTGTGGCGTTGTATGACTTCCAGGAGATGGCCAATTCTGTCTACCTGGTCATGGAGTATTGTAATGGTGGAGACCTGGCTGACTACCTGCACACTATGCGCACACTGAGTGAAGACACTGTCAGGCTGTTCCTACAACAGATTGCAGGTGCCATGCAGCTGCTACACAGCAAGGGCATCATTCACCGTGATTTGAAGCCCCAGAACATCCTGCTGTCCAATCCCGGGGGTCGACGTGCCAACCCCAGCAACATCCGAGTCAAGATTGCTGACTTTGGATTTGCCCGGTACCTCCAGAGCAACATGATGGCGGCCACGCTCTGTGGCTCTCCTATGTACATGGCCCCTGAGGTTATCATGTCCCAGCACTACGATGGGAAGGCCGATCTATGGAGTATCGGTACTATTGTCTACCAGTGTCTGACAGGGAAGGCCCCTTTTCAGGCCAGCAGCCCTCAGGATTTGCGCCTGTTTTATGAGAAGAACAAGACACTAGTTCCTGCCATCCCACGGGAGACATCAGCTCCCCTGCGGCAGTTGCTTCTGGCTCTATTGCAGCGTAACCACAAGGACCGCATGGACTTTGATGAATTTTTCCACCACCCTTTCTTAGATGCCAGCACCCCCATCAAGAAATCCCCACCTGTGCCTGTGCCCTCATATCCAAGCTCAGGGTCCGGCAGCAGCTCCAGCAGCAGCTCTGCCTCCCACCTGGCATCTCCCCCGTCCCTGGGGGAGATGCCGCAACTGCAGAAGACCCTTACCTCCCCAGCTGATGCTGCTGGCTTTCTGCAGGGGTCCCGGGACTCTGGTGGCAGTAGCAAAGACTCCTGTGACACAGATGACTTTGTCATGGTCCCAGCCCAGTTTCCAGGTGATCTAGTCCCTGAGGCAGCCAGTGCCAAGCCCCCACCTGACAGTCTGCTGTGTAGTGGGAGCTCACTGGTGGCCTCTGCTGGCCTAGAGAGCCATGGCCGTACCCCATCTCCCTCTCCGACCTGCAGCAGCTCTCCCAGCCCCTCTGGCCGGCCTGGCCCCTTCTCCAGCAACAGGTACGGTGCCTCGGTCCCCATTCCTGTCCCCACTCAGGTGCACAACTACCAGCGCATCGAGCAGAACCTGCAGTCACCTACTCAACAGCAGACAGCCCGGTCTTCTGCCATCCGAAGGTCAGGTAGCACCAGCCCCCTGGGCTTTGGTCGGGCCAGCCCATCACCTCCCTCGCACACTGATGGAGCCATGCTGGCCAGGAAGCTGTCACTAGGAGGTGGCCGTCCCTACACACCCTCTCCCCAAGTGGGAACCATCCCAGAGCGGCCCAGCTGGAGCAGAGTACCCTCCCCACAAGGAGCTGATGTGCGGGCTGGCAGGTCACCACGACCAGGCTCCTCTGTGCCTGAGCACTCTCCACGAACCACCGGGCTGGGCTGCCGCCTGCACAGTGCCCCCAACCTTTCGGACTTCCATGTCGTTCGTCCCAAGCTGCCTAAGCCTCCAACAGACCCACTGGGAGCTACCTTCAGCCCACCCCAGACCAGCGCACCCCAGCCATGCCCAGGGCTACAGTCTTGCCGGCCACTGCGTGGCTCACCTAAGCTGCCTGACTTCCTACAGCGGAGTCCCCTACCCCCCATCCTAGGCTCTCCTACTAAGGCTGGACCCTCGTTTGACTTCCCCAAAGCCCCCAGCTCTCAGAGTTTGCTGACCCTGTTGGCTAGGCAGGGTGTAGTAATGACACCACCTCGGAACCGTACACTGCCTGACCTCTCAGAGGCGGGTCCTTTCCAGGGCCAGCAGCTGGGCTCTGGCCTACGGCCCGCCGAAGACACAAGGGGCCCCTTTGGCCGGTCCTTCAGCACCAGCCGCCTTACGGACCTGCTGCTTAAGGCTGCATTTGGGACACAGGCCTCTGACTCAGGCAGCACTGACAGCCTACAGGAGAAACCTATGGAGATTGCTCCCTCTGCTGGTTTTGGAGGGACTCTGCATCCAGGAGCTCGTGCTGGAGGGGCAAGCAGCCCAGCACCTGTGGTATTTACTGTGGGCTCCCCACCCAGTGGTACCACCCCACCCCAGAGTACCCGCACCAGAATGTTCTCAGTGGGCTCTTCCAGCTCCCTGGGCTCGACTGGCTCCTCCTCTGCCCGCCACTTAGTGCCTGGGGCTTGTGCAGAGGCCCCGGAGCTTTCTGCCCCAGGCCACTGCTGTAGCCTTGCTGACCCCCTTGCTGCCAACTTGGAGGGGGCTGTGACCTTCGAGGCTCCTGACCTCCCAGAGGAGACCCTCATGGAGCAAGAGCACACGGAAACCCTACACAGCCTGCGCTTCACACTAGCGTTTGCACAGCAAGTTCTGGAGATTGCAGCCCTGAAGGGCAGTGCCAGTGAGGCAGCCGGGGGCCCTGAGTACCAGCTTCAGGAAAGTGTGGTGGCTGACCAGATCAGTCAGTTGAGCCGAGAGTGGGGCTTTGCAGAGCAACTGGTTCTGTACTTGAAGGTGGCTGAGCTGCTCTCTTCAGGCTTACAGACTGCCATTGACCAGATTCGAGCTGGCAAGCTCTGCCTTTCGTCTACTGTGAAGCAGGTGGTACGCAGACTAAATGAGCTGTATAAGGCCAGCGTGGTATCCTGCCAGGGCCTCAGTCTGCGGCTTCAGCGCTTCTTTCTGGACAAGCAACGGCTGCTGGATGGGATCCATGGTGTCACTGCAGAGCGGCTTATCCTCAGCCATGCTGTGCAGATGGTACAATCAGCTGCACTCGATGAGATGTTCCAGCACCGAGAGAGCTGTGTACCTCGATATCACAAAGCCCTGCTGTTGTTGGAGGGGCTGCAGCACACTCTCACGGACCAGGCAGACATTGAGAACATTGCCAAATGCAAGCTGTGCATTGAGAGGAGACTCTCGGCCCTGCTGAGTGGCGTCTATGCCTGAGCGGCCGC

Amino Acid Sequence

MEPGRGGVETVGKFEFSRKDLIGHGAFAVVFKGRHREKHDLEVAVKCINKKNLAKSQTLLGKEIKILKELKHENIVALYDFQEMANSVYLVMEYCNGGDLADYLHTMRTLSEDTVRLFLQQIAGAMQLLHSKGIIHRDLKPQNILLSNPGGRRANPSNIRVKIADFGFARYLQSNMMAATLCGSPMYMAPEVIMSQHYDGKADLWSIGTIVYQCLTGKAPFQASSPQDLRLFYEKNKTLVPAIPRETSAPLRQLLLALLQRNHKDRMDFDEFFHHPFLDASTPIKKSPPVPVPSYPSSGSGSSSSSSSASHLASPPSLGEMPQLQKTLTSPADAAGFLQGSRDSGGSSKDSCDTDDFVMVPAQFPGDLVPEAASAKPPPDSLLCSGSSLVASAGLESHGRTPSPSPTCSSSPSPSGRPGPFSSNRYGASVPIPVPTQVHNYQRIEQNLQSPTQQQTARSSAIRRSGSTSPLGFGRASPSPPSHTDGAMLARKLSLGGGRPYTPSPQVGTIPERPSWSRVPSPQGADVRAGRSPRPGSSVPEHSPRTTGLGCRLHSAPNLSDFHVVRPKLPKPPTDPLGATFSPPQTSAPQPCPGLQSCRPLRGSPKLPDFLQRSPLPPILGSPTKAGPSFDFPKAPSSQSLLTLLARQGVVMTPPRNRTLPDLSEAGPFQGQQLGSGLRPAEDTRGPFGRSFSTSRLTDLLLKAAFGTQASDSGSTDSLQEKPMEIAPSAGFGGTLHPGARAGGASSPAPVVFTVGSPPSGTTPPQSTRTRMFSVGSSSSLGSTGSSSARHLVPGACAEAPELSAPGHCCSLADPLAANLEGAVTFEAPDLPEETLMEQEHTETLHSLRFTLAFAQQVLEIAALKGSASEAAGGPEYQLQESVVADQISQLSREWGFAEQLVLYLKVAELLSSGLQTAIDQIRAGKLCLSSTVKQVVRRLNELYKASVVSCQGLSLRLQRFFLDKQRLLDGIHGVTAERLILSHAVQMVQSAALDEMFQHRESCVPRYHKALLLLEGLQHTLTDQADIENIAKCKLCIERRLSALLSGVYA*

• Vector Type: Mammalian
• DU Number: DU59026
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: rat ULK1 (shRNA resistant, AAGGCTGACCTGTGGAGCATTGGCACCAT) into pBabe-hygro-FLAG vector. Thanks.
• Low Temp: No