cDNA Clone ZUFSP

• Plasmid: pcDNA FRT TO GFP 5xGS ZUFSP 187-end
• Parent Plasmid: pcDNA FRT TO GFP
• Expressed: GFP 5xGS ZUFSP 187-end
• Tag: GFP

Sequence of Insert

GGATCCGGTTCTGGTAGTGGTTCCGGTTCCGGTTCTGGATCCATGGACTGTGATCAACCACTCTATGATTGTCCTATGTGTGGGCTCATATGTACAAATTACCATATTCTTCAGGAACATGTTGACTTGCATTTGGAAGAAAACAGCTTTCAGCAAGGCATGGATAGAGTCCAGTGTTCTGGTGATCTACAATTGGCTCACCAGCTTCAGCAAGAAGAAGACAGAAAGAGGAGATCTGAAGAATCAAGACAAGAAATAGAAGAATTTCAGAAGCTGCAGAGACAATATGGTTTAGATAATTCTGGAGGATACAAACAACAACAACTACGAAATATGGAGATAGAAGTAAATAGGGGAAGAATGCCTCCATCTGAATTTCATAGGAGAAAAGCTGATATGATGGAATCATTAGCTCTTGGTTTTGACGATGGAAAAACAAAAACTTCCGGAATTATTGAAGCACTTCATAGGTATTATCAGAATGCTGCCACAGATGTGAGACGGGTGTGGCTTTCTTCAGTGGTGGATCACTTTCATTCATCTTTAGGCGACAAAGGTTGGGGTTGTGGTTACAGAAATTTCCAAATGCTACTTTCATCATTATTACAAAATGATGCTTACAACGATTGCTTAAAAGGTATGTTGATTCCTTGCATTCCAAAAATTCAATCTATGATTGAAGATGCATGGAAGGAAGGTTTTGATCCTCAGGGGGCCTCTCAACTTAATAACAGGTTACAGGGAACAAAGGCCTGGATTGGAGCATGTGAAGTATATATACTCCTGACCTCCCTAAGGGTAAAGTGTCATATTGTTGATTTTCACAAATCAACTGGTCCTTTGGGTACACACCCTCGCTTATTTGAATGGATATTGAACTATTATTCTTCAGAGGGAGAAGGGAGTCCAAAGGTAGTGTGTACATCTAAACCTCCTATCTATCTTCAGCATCAAGGTCACAGTCGAACTGTTATTGGAATTGAAGAGAAAAAAAACCGAACATTATGCTTACTAATACTTGATCCTGGATGTCCTTCTCGAGAAATGCAGAAATTATTAAAGCAAGACATAGAGGCTAGCAGTCTCAAGCAACTTCGGAAATCTATGGGAAATTTAAAACATAAGCAATACCAGATATTGGCAGTAGAGGGTGCTCTTTCTCTAGAGGAGAAACTTGCCAGGAGACAAGCTTCTCAAGTCTTTACAGCCGAGAAGATTCCTTGAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSGSGSGSGSGSGSMDCDQPLYDCPMCGLICTNYHILQEHVDLHLEENSFQQGMDRVQCSGDLQLAHQLQQEEDRKRRSEESRQEIEEFQKLQRQYGLDNSGGYKQQQLRNMEIEVNRGRMPPSEFHRRKADMMESLALGFDDGKTKTSGIIEALHRYYQNAATDVRRVWLSSVVDHFHSSLGDKGWGCGYRNFQMLLSSLLQNDAYNDCLKGMLIPCIPKIQSMIEDAWKEGFDPQGASQLNNRLQGTKAWIGACEVYILLTSLRVKCHIVDFHKSTGPLGTHPRLFEWILNYYSSEGEGSPKVVCTSKPPIYLQHQGHSRTVIGIEEKKNRTLCLLILDPGCPSREMQKLLKQDIEASSLKQLRKSMGNLKHKQYQILAVEGALSLEEKLARRQASQVFTAEKIP*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU55841
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Hi, please can I get ZUFSP 187-578 into pcDNA5 FRT/TO with a N-term eGFP-GS(x6) linker (I have used the sequence GGATCCGGTTCTGGTAGTGGTTCCGGTTCCGGTTCT for the linker previously if it helps any). Cheers
• Low Temp: No