cDNA Clone CEP131

• Synonyms: AZ1; AZI1; ZA1
• Plasmid: pET15 6His MBP TEV CEP131
• Parent Plasmid: pET15 6HIS MBP DU
• Expressed: 6His MBP TEV CEP131
• Genbank: NM_001319228.1 GI:984880705*
• Tag: 6His
• Tag: MBP
• Species: Human

Sequence of Insert

GGATCCATGAAAGGCACCCGGGCCATCGGCAGCGTCCCGGAGCGCAGCCCAGCAGGTGTGGACCTGAGTCTGACAGGTCTCCCTCCGCCTGTGTCCCGGCGTCCTGGCAGTGCCGCCACCACCAAGCCCATCGTCCGCTCTGTCTCCGTGGTCACAGGCAGCGAGCAGAAGAGGAAGGTGCTGGAGGCCACAGGGCCTGGGGGCTCCCAGGCCATCAACAACCTTAGAAGATCCAACAGCACCACGCAGGTCAGCCAGCCTCGGAGCGGCTCCCCCAGGCCAACGGAGCCCACAGACTTCCTGATGCTCTTCGAGGGCAGCCCCAGTGGGAAAAAGAGGCCTGCCAGCCTGAGCACAGCCCCCAGCGAGAAGGGAGCCACCTGGAACGTCCTGGATGACCAGCCCCGGGGCTTCACCTTGCCATCCAATGCCCGGAGTTCCAGTGCCCTTGACTCACCAGCGGGCCCGCGGAGGAAAGAATGCACCGTGGCCCTGGCCCCCAACTTCACTGCTAACAACAGGAGCAACAAGGGAGCAGTGGGCAACTGCGTCACCACCATGGTGCACAACCGCTACACCCCCTCGGAGAGGGCGCCTCCGCTCAAGAGCTCCAACCAGACTGCCCCCTCCCTCAACAACATCATCAAGGCAGCCACCTGTGAGGGCAGTGAGAGCAGCGGCTTTGGGAAGCTGCCGAAGAATGTCTCCAGTGCCACCCACTCAGCCCGGAACAATACTGGGGGCAGCACGGGCTTGCCCAGGCGGAAGGAGGTGACGGAGGAGGAGGCTGAGAGGTTTATCCACCAGGTGAACCAGGCCGCTGTCACCATCCAGCGCTGGTACCGGCACCAGGTGCAGCGGCGCGGAGCAGGAGCTGCCCGCCTGGAGCACTTGCTTCAGGCCAAGCGGGAGGAGCAGCGGCAGCGGTCAGGCGAGGGGACCCTCTTGGACCTGCACCAGCAGAAAGAGGCAGCCAGGAGGAAGGCCCGGGAGGAGAAGGCACGCCAAGCCAGGCGAGCAGCCATTCAGGAGCTGCAACAGAAACGAGCCCTGAGAGCCCAGAAGGCGAGCACTGCCGAGCGTGGGCCACCTGAGAATCCCAGGGAGACCAGAGTGCCAGGAATGCGGCAGCCTGCTCAGGAGCTGTCCCCCACGCCAGGCGGCACTGCCCACCAGGCCCTCAAGGCCAACAATGCTGGTGGTGGCCTCCCTGCTGCAGGCCCCGGAGACCGCTGCCTGCCCACCTCCGACTCATCCCCAGAACCACAGCAGCCTCCAGAGGACAGGACGCAGGACGTTCTTGCCCAGGATGCAGCTGGGGACAACCTGGAGATGATGGCCCCGAGCAGGGGGAGCGCCAAGTCCAGGGGGCCACTGGAGGAGCTGCTGCACACACTGCAGCTGCTGGAGAAGGAGCCGGACGCGCTGCCCCGCCCCAGGACCCATCACAGGGGCAGATACGCCTGGGCCAGCGAGGTGACCACGGAGGATGACGCCAGCTCTCTGACAGCTGACAACTTGGAGAAATTTGGAAAACTCAGTGCGTTCCCCGAACCTCCTGAGGATGGGACGCTGCTATCGGAGGCCAAGCTACAAAGCATCATGAGCTTCTTGGACGAGATGGAGAAGTCTGGGCAGGACCAGCTGGACTCCCAGCAGGAGGGGTGGGTGCCGGAGGCGGGGCCGGGGCCCCTGGAGCTGGGGTCCGAGGTGAGCACGTCTGTGATGCGGCTGAAGCTGGAGGTGGAGGAGAAGAAGCAGGCCATGCTGCTGCTGCAGAGAGCGCTGGCGCAGCAGCGAGACCTCACGGCCCGGCGGGTCAAGGAGACAGAGAAGGCACTGAGCCGGCAGCTGCAGCGGCAGAGGGAGCACTACGAGGCCACCATCCAGCGGCACTTGGCCTTCATTGACCAGCTGATTGAGGACAAGAAGGTCCTGAGTGAAAAGTGCGAGGCTGTGGTGGCCGAGCTGAAGCAGGAGGACCAGAGATGCACCGAGCGTGTGGCCCAGGCACAGGCGCAGCACGAGCTGGAGATTAAAAAACTCAAAGAATTAATGAGCGCCACCGAGAAAGCCCGCCGGGAGAAGTGGATCAGTGAGAAAACCAAGAAGATCAAGGAGGTCACTGTCCGAGGTCTGGAGCCCGAGATCCAGAAGCTGATTGCAAGGCACAAGCAGGAAGTGCGGAGGCTCAAGAGCCTGCACGAGGCGGAGCTGCTGCAGTCGGATGAGCGGGCCTCGCAGCGCTGCCTGCGCCAGGCCGAGGAGCTGCGGGAGCAGCTGGAGCGGGAGAAGGAGGCGCTGGGCCAGCAGGAGCGCGAACGTGCTCGGCAGCGGTTCCAGCAGCACCTGGAGCAGGAGCAGTGGGCGCTGCAGCAGCAACGGCAGCGGCTGTACAGTGAGGTGGCTGAGGAGAGGGAGCGGCTGGGCCAGCAGGCAGCCAGGCAGCGGGCGGAGCTGGAAGAGCTGAGGCAGCAGCTGGAGGAGAGCAGCTCTGCACTGACCCGAGCCCTGAGGGCTGAGTTTGAGAAGGGCAGGGAGGAGCAGGAGCGCCGGCACCAGATGGAGCTGAATACCCTGAAGCAGCAGCTGGAGCTGGAGAGGCAGGCGTGGGAGGCCGGCCGCACCAGGAAGGAGGAGGCGTGGCTGCTGAACCGGGAACAGGAGCTGAGGGAAGAAATCCGGAAAGGCCGGGACAAGGAGATTGAGCTGGTCATTCACCGGCTGGAGGCCGACATGGCGCTGGCCAAGGAGGAGAGTGAGAAGGCTGCCGAGAGCCGCATCAAGCGCTTACGGGACAAGTACGAGGCCGAGCTCTCCGAGCTGGAGCAGTCGGAGCGGAAGCTTCAGGAGCGGTGCTCGGAGCTGAAGGGCCAGCTTGGGGAGGCCGAGGGCGAGAATCTGCGTCTGCAGGGCCTTGTGCGGCAGAAGGAGCGGGCGCTGGAGGATGCGCAGGCGGTGAACGAGCAGCTTTCTAGCGAGCGCAGCAACCTGGCCCAGGTGATCCGCCAGGAGTTCGAGGACCGGCTGGCAGCCTCTGAGGAGGAGACGCGGCAGGCCAAGGCCGAGCTGGCCACGCTGCAGGCCCGCCAGCAGCTGGAGCTGGAGGAGGTGCACCGGAGGGTGAAGACAGCCCTCGCGAGGAAGGAGGAGGCCGTGAGCAGCCTCCGGACACAACATGAGGCTGCGGTGAAGCGGGCCGACCACCTGGAGGAGCTGCTGGAGCAGCACAGGAGGCCCACGCCAAGTACCAAGTGAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHGSSMKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQ
DKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNA
GAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLT
DEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNNNNNNNNLGENLY
FQGHMGSMKGTRAIGSVPERSPAGVDLSLTGLPPPVSRRPGSAATTKPIVRSVSVVTGSEQKRKVLEATGPGGSQAINNLRRSNSTTQVSQPRSGSPRPT
EPTDFLMLFEGSPSGKKRPASLSTAPSEKGATWNVLDDQPRGFTLPSNARSSSALDSPAGPRRKECTVALAPNFTANNRSNKGAVGNCVTTMVHNRYTPS
ERAPPLKSSNQTAPSLNNIIKAATCEGSESSGFGKLPKNVSSATHSARNNTGGSTGLPRRKEVTEEEAERFIHQVNQAAVTIQRWYRHQVQRRGAGAARL
EHLLQAKREEQRQRSGEGTLLDLHQQKEAARRKAREEKARQARRAAIQELQQKRALRAQKASTAERGPPENPRETRVPGMRQPAQELSPTPGGTAHQALK
ANNAGGGLPAAGPGDRCLPTSDSSPEPQQPPEDRTQDVLAQDAAGDNLEMMAPSRGSAKSRGPLEELLHTLQLLEKEPDALPRPRTHHRGRYAWASEVTT
EDDASSLTADNLEKFGKLSAFPEPPEDGTLLSEAKLQSIMSFLDEMEKSGQDQLDSQQEGWVPEAGPGPLELGSEVSTSVMRLKLEVEEKKQAMLLLQRA
LAQQRDLTARRVKETEKALSRQLQRQREHYEATIQRHLAFIDQLIEDKKVLSEKCEAVVAELKQEDQRCTERVAQAQAQHELEIKKLKELMSATEKARRE
KWISEKTKKIKEVTVRGLEPEIQKLIARHKQEVRRLKSLHEAELLQSDERASQRCLRQAEELREQLEREKEALGQQERERARQRFQQHLEQEQWALQQQR
QRLYSEVAEERERLGQQAARQRAELEELRQQLEESSSALTRALRAEFEKGREEQERRHQMELNTLKQQLELERQAWEAGRTRKEEAWLLNREQELREEIR
KGRDKEIELVIHRLEADMALAKEESEKAAESRIKRLRDKYEAELSELEQSERKLQERCSELKGQLGEAEGENLRLQGLVRQKERALEDAQAVNEQLSSER
SNLAQVIRQEFEDRLAASEEETRQAKAELATLQARQQLELEEVHRRVKTALARKEEAVSSLRTQHEAAVKRADHLEELLEQHRRPTPSTK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU58306
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: *Three residue changes compared with NM_001319228.1 CDS: T272A, T397A, V473A (The cloned sequence matches EAW89632.1. Changes are RNA-derived and also seen in EST sequences. Also, no tryptic peptides were identified around these residues by Mass-spec, although there are R/K residues that should have given peptides. This suggests that what was obtained from RNA sources could very well be the dominant isoform in cells.)
• Low Temp: No