cDNA Clone FAM83H

• Plasmid: pBabeD puro nVenus(I152L)-5Gly-HA-FAM83H
• Parent Plasmid: pBabeD puro
• Expressed: nVenus(I152L)-5Gly-HA-FAM83H
• Genbank: NM_198488.3
• Tag: HA
• Tag: YFP
• Species: Human

Sequence of Insert

AGATCTATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCAT
CCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCG
GCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGCTGATC
TGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCT
GGGCTACGGCCTGCAGTGCTTCGCCCGCTACCCCGACCACATGAAGCAGC
ACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACC
ATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTT
CGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCA
AGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGC
CACAACGTCTATCTCACCGCCGGTGGCGGAGGTGGCATGTACCCATACGA
TGTTCCAGATTACGCTGGATCC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKLICT
TGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYLTAGGGGGMYPYDVPDYAGSMARRSQSSSQGDNPLAPGYLPPHYKEYY
RLAVDALAEGGSEAYSRFLATEGAPDFLCPEELEHVSRHLRPPQYVTREP
PEGSLLDVDMDGSSGTYWPVNSDQAVPELDLGWPLTFGFQGTEVTTLVQP
PPPDSPSIKDEARRMIRSAQQVVAVVMDMFTDVDLLSEVLEAAARRVPVY
ILLDEMNAQHFLDMADKCRVNLHHVDFLRVRTVAGPTYYCRTGKSFKGHV
KEKFLLVDCAVVMSGSYSFMWSFEKIHRSLAHVFQGELVSSFDEEFRILF
AQSEPLVPSAAALARMDAYALAPYAGAGPLVGVPGVGAPTPFSFPKRAHL
LFPPPREEGLGFPSFLDPDRHFLSAFRREEPPRMPGGALEPHAGLRPLSR
RLEAEAGPAGELAGARGFFQARHLEMDAFKRHSFATEGAGAVENFAAARQ
VSRQTFLSHGDDFRFQTSHFHRDQLYQQQYQWDPQLTPARPQGLFEKLRG
GRAGFADPDDFTLGAGPRFPELGPDGHQRLDYVPSSASREVRHGSDPAFA
PGPRGLEPSGAPRPNLTQRFPCQAAARPGPDPAPEAEPERRGGPEGRAGL
RRWRLASYLSGCHGEDGGDDGLPAPMEAEAYEDDVLAPGGRAPAGDLLPS
AFRVPAAFPTKVPVPGPGSGGNGPEREGPEEPGLAKQDSFRSRLNPLVQR
SSRLRSSLIFSTSQAEGAAGAAAATEKVQLLHKEQTVSETLGPGGEAVRS
AASTKVAELLEKYKGPARDPGGGAGAITVASHSKAVVSQAWREEVAAPGA
VGGERRSLESCLLDLRDSFAQQLHQEAERQPGAASLTAAQLLDTLGRSGS
DRLPSRFLSAQSHSTSPQGLDSPLPLEGSGAHQVLHNESKGSPTSAYPER
KGSPTPGFSTRRGSPTTGFIEQKGSPTSAYPERRGSPVPPVPERRSSPVP
PVPERRGSLTLTISGESPKAGPAEEGPSGPMEVLRKGSLRLRQLLSPKGE
RRMEDEGGFPVPQENGQPESPRRLSLGQGDSTEAATEERGPRARLSSATA
NALYSSNLRDDTKAILEQISAHGQKHRAVPAPSPGPTHNSPELGRPPAAG
VLAPDMSDKDKCSAIFRSDSLGTQGRLSRTLPASAEERDRLLRRMESMRK
EKRVYSRFEVFCKKEEASSPGAGEGPAEEGTRDSKVGKFVPKILGTFKSK
K*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU29033
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Split Venus YFP - comprises N-terminal 155 residues (M1-A155) followed by a 5 gly linker and an HA tag. Venus sequence contains mutation to reduce dimerisation and aggregation - listed as I152L Addgene nVenus plasmid pBIFC-VN155 but in this clone refers to L153.; Fam83H has silent changes at Pro375 and Ala425 compared to NCBI ref. ; Parental 41279 (33769 backbone)
• Low Temp: No