cDNA Clone FAM83D

• Plasmid: pcDNA5-FRT/TO-GFP-FAM83D T370A/T543A
• Parent Plasmid: pGEX 6P-1
• Expressed: GFP-FAM83D T370A/T543A
• Genbank: NM_030919.2
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCATGGCGCGGGCCTGTTTGATTCAAAGGTTGCCTATAAAGCGGGACTGCACGCCGGTTTTTGTCCGAGGGCTGTCGAGTCCGAGCGCCGCCATGGCTCTGCTGTCCGAGGGCCTGGACGAGGTGCCCGCCGCCTGCCTGTCGCCGTGCGGGCCGCCCAACCCGACCGAGCTGTTCAGCGAGTCACGGCGCCTGGCTCTGGAGGAGCTGGTGGCGGGCGGCCCCGAAGCCTTCGCGGCCTTCCTGCGACGCGAGCGCCTGGCTCGTTTCCTGAACCCCGATGAGGTGCACGCCATTCTGCGCGCGGCGGAGAGGCCGGGAGAGGAGGGCGCGGCGGCGGCGGCGGCGGCCGAGGACTCGTTCGGCTCCTCGCACGACTGCTCTTCGGGCACCTACTTCCCCGAGCAGTCGGACCTGGAGCCACCGCTGTTGGAGCTTGGCTGGCCCGCCTTCTACCAGGGCGCCTACCGCGGCGCCACGCGTGTCGAGACGCACTTCCAGCCCCGCGGCGCTGGCGAAGGTGGCCCCTACGGCTGCAAGGACGCTCTGCGCCAGCAGCTCCGCTCGGCGCGAGAGGTGATTGCAGTGGTCATGGACGTGTTCACAGACATCGACATCTTCAGAGACCTGCAAGAAATATGCAGGAAACAGGGAGTTGCTGTGTATATCCTTCTGGACCAGGCTCTCCTCTCTCAATTTCTGGATATGTGCATGGATCTGAAAGTTCATCCTGAACAGGAAAAGTTAATGACAGTTCGGACTATCACAGGAAATATCTACTATGCAAGGTCAGGAACTAAGATTATTGGGAAGGTTCACGAAAAGTTCACGTTGATTGATGGCATCCGCGTGGCAACAGGCTCCTACAGTTTTACATGGACGGATGGCAAATTAAACAGCAGTAACTTGGTAATTCTGTCTGGCCAAGTGGTTGAACACTTTGATCTGGAATTCCGAATCCTGTATGCCCAGTCCAAGCCCATCAGCCCCAAACTCCTGTCTCACTTCCAGAGCAGCAACAAGTTTGATCACCTCACCAACCGAAAACCACAGTCCAAGGAGCTCACCCTGGGCAACCTGCTGCGGATGCGGCTGGCTAGGCTGTCAAGTGCTCCCAGGAAGGCGGACCTGGACCCAGAGATGCCCGCAGAGGGCAAGGCAGAGCGCAAGCCCCATGACTGTGAGTCCTCTACTGTTAGTGAGGAAGACTACTTCAGCAGCCACAGGGACGAGCTCCAGAGCAGAAAGGCCATTGACGCTGCCACTCAAACAGAGCCAGGAGAGGAGATGCCAGGGCTGAGTGTGAGTGAGGTGGGAACACAAACCAGCATCACCACAGCATGTGCTGGTACCCAGACTGCAGTCATCACCAGGATAGCAAGCTCTCAAACCACGATTTGGTCCAGATCGACCACTACTCAGACTGACATGGATGAGAACATTCTCTTTCCTCGAGGAACTCAATCTACAGAAGGGTCACCAGTCTCAAAAATGTCTGTATCGAGATCTTCCAGTTTGAAGTCTTCCTCCTCTGTGTCTTCCCAAGGCTCTGTGGCAAGCTCCACTGGTTCTCCCGCTTCCATCAGAACCACTGACTTCCACAATCCTGGCTATCCCAAGTACCTGGGCGCCCCCCACCTGGAACTGTACTTGAGTGACTCACTTAGAAACTTGAACAAAGAGCGGCAATTCCACTTCGCTGGTATCAGGTCCCGGCTCAACCACATGCTGGCTATGCTGTCAAGGAGAACACTCTTTACTGAAAACCACCTTGGCCTTCATTCTGGCAATTTCAGCAGAGTTAATTTGCTTGCTGTTAGAGATGTAGCACTTTATCCTTCCTATCAGTAAGTCGACTCGAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMARACL
IQRLPIKRDCTPVFVRGLSSPSAAMALLSEGLDEVPAACLSPCGPPNPTE
LFSESRRLALEELVAGGPEAFAAFLRRERLARFLNPDEVHAILRAAERPG
EEGAAAAAAAEDSFGSSHDCSSGTYFPEQSDLEPPLLELGWPAFYQGAYR
GATRVETHFQPRGAGEGGPYGCKDALRQQLRSAREVIAVVMDVFTDIDIF
RDLQEICRKQGVAVYILLDQALLSQFLDMCMDLKVHPEQEKLMTVRTITG
NIYYARSGTKIIGKVHEKFTLIDGIRVATGSYSFTWTDGKLNSSNLVILS
GQVVEHFDLEFRILYAQSKPISPKLLSHFQSSNKFDHLTNRKPQSKELTL
GNLLRMRLARLSSAPRKADLDPEMPAEGKAERKPHDCESSTVSEEDYFSS
HRDELQSRKAIDAATQTEPGEEMPGLSVSEVGTQTSITTACAGTQTAVIT
RIASSQTTIWSRSTTTQTDMDENILFPRGTQSTEGSPVSKMSVSRSSSLK
SSSSVSSQGSVASSTGSPASIRTTDFHNPGYPKYLGAPHLELYLSDSLRN
LNKERQFHFAGIRSRLNHMLAMLSRRTLFTENHLGLHSGNFSRVNLLAVR
DVALYPSYQ*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU29102
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Can excise BamHI-Not or BamHI-Sal1. Silent change at Glu316 (GAG > GAA)
• Low Temp: No