cDNA Clone FAM83D

• Plasmid: pcDNA5-FRT/TO-GFP-FAM83D T513E
• Parent Plasmid: pcDNA5-FRT/TO-GFP
• Expressed: GFP-FAM83D T513E
• Tag: GFP
• Species: Human

Sequence of Insert

ggatccATGGCTCTGCTGTCCGAGGGCCTGGACGAGGTGCCCGCCGCCTG
CCTGTCGCCGTGCGGGCCGCCCAACCCGACCGAGCTGTTCAGCGAGTCAC
GGCGCCTGGCTCTGGAGGAGCTGGTGGCGGGCGGCCCCGAAGCCTTCGCG
GCCTTCCTGCGACGCGAGCGCCTGGCTCGTTTCCTGAACCCCGATGAGGT
GCACGCCATTCTGCGCGCGGCGGAGAGGCCGGGAGAGGAGGGCGCGGCGG
CGGCGGCGGCGGCCGAGGACTCGTTCGGCTCCTCGCACGACTGCTCTTCG
GGCACCTACTTCCCCGAGCAGTCGGACCTGGAGCCACCGCTGTTGGAGCT
TGGCTGGCCCGCCTTCTACCAGGGCGCCTACCGCGGCGCCACGCGTGTCG
AGACGCACTTCCAGCCCCGCGGCGCTGGCGAAGGTGGCCCCTACGGCTGC
AAGGACGCTCTGCGCCAGCAGCTCCGCTCGGCGCGAGAGGTGATTGCAGT
GGTCATGGACGTGTTCACAGACATCGACATCTTCAGAGACCTGCAAGAAA
TATGCAGGAAACAGGGAGTTGCTGTGTATATCCTTCTGGACCAGGCTCTC
CTCTCTCAATTTCTGGATATGTGCATGGATCTGAAAGTTCATCCTGAACA
GGAAAAGTTAATGACAGTTCGGACTATCACAGGAAATATCTACTATGCAA
GGTCAGGAACTAAGATTATTGGGAAGGTTCACGAAAAGTTCACGTTGATT
GATGGCATCCGCGTGGCAACAGGCTCCTACAGTTTTACATGGACGGATGG
CAAATTAAACAGCAGTAACTTGGTAATTCTGTCTGGCCAAGTGGTTGAAC
ACTTTGATCTGGAATTCCGAATCCTGTATGCCCAGTCCAAGCCCATCAGC
CCCAAACTCCTGTCTCACTTCCAGAGCAGCAACAAGTTTGATCACCTCAC
CAACCGAAAACCACAGTCCAAGGAGCTCACCCTGGGCAACCTGCTGCGGA
TGCGGCTGGCTAGGCTGTCAAGTACTCCCAGGAAGGCGGACCTGGACCCA
GAGATGCCCGCAGAGGGCAAGGCAGAGCGCAAGCCCCATGACTGTGAGTC
CTCTACTGTTAGTGAGGAAGACTACTTCAGCAGCCACAGGGACGAGCTCC
AGAGCAGAAAGGCCATTGACGCTGCCACTCAAACAGAGCCAGGAGAGGAG
ATGCCAGGGCTGAGTGTGAGTGAGGTGGGAACACAAACCAGCATCACCAC
AGCATGTGCTGGTACCCAGACTGCAGTCATCACCAGGATAGCAAGCTCTC
AAACCACGATTTGGTCCAGATCGACCACTACTCAGACTGACATGGATGAG
AACATTCTCTTTCCTCGAGGAACTCAATCTACAGAAGGGTCACCAGTCTC
AAAAATGTCTGTATCGAGATCTTCCAGTTTGAAGTCTTCCTCCTCTGTGT
CTTCCCAAGGCTCTGTGGCAAGCTCCACTGGTTCTCCCGCTTCCATCAGA
ACCACTGACTTCCACAATCCTGGCTATCCCAAGTACCTGGGCgagCCCCA
CCTGGAACTGTACTTGAGTGACTCACTTAGAAACTTGAACAAAGAGCGGC
AATTCCACTTCGCTGGTATCAGGTCCCGGCTCAACCACATGCTGGCTATG
CTGTCAAGGAGAACACTCTTTACTGAAAACCACCTTGGCCTTCATTCTGG
CAATTTCAGCAGAGTTAATTTGCTTGCTGTTAGAGATGTAGCACTTTATC
CTTCCTATCAGTAAgtcgacTCGCTCGAGAGTGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMALLSE
GLDEVPAACLSPCGPPNPTELFSESRRLALEELVAGGPEAFAAFLRRERL
ARFLNPDEVHAILRAAERPGEEGAAAAAAAEDSFGSSHDCSSGTYFPEQS
DLEPPLLELGWPAFYQGAYRGATRVETHFQPRGAGEGGPYGCKDALRQQL
RSAREVIAVVMDVFTDIDIFRDLQEICRKQGVAVYILLDQALLSQFLDMC
MDLKVHPEQEKLMTVRTITGNIYYARSGTKIIGKVHEKFTLIDGIRVATG
SYSFTWTDGKLNSSNLVILSGQVVEHFDLEFRILYAQSKPISPKLLSHFQ
SSNKFDHLTNRKPQSKELTLGNLLRMRLARLSSTPRKADLDPEMPAEGKA
ERKPHDCESSTVSEEDYFSSHRDELQSRKAIDAATQTEPGEEMPGLSVSE
VGTQTSITTACAGTQTAVITRIASSQTTIWSRSTTTQTDMDENILFPRGT
QSTEGSPVSKMSVSRSSSLKSSSSVSSQGSVASSTGSPASIRTTDFHNPG
YPKYLGEPHLELYLSDSLRNLNKERQFHFAGIRSRLNHMLAMLSRRTLFT
ENHLGLHSGNFSRVNLLAVRDVALYPSYQ*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU29116
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: delta aaM1-Ala30 with respect to full-length NCBI ref. ; N-terminally truncated predicted variant. Silent change at Glu316 (GAG > GAA); Parental 41455
• Low Temp: No