cDNA Clone Esc4 (yeast)

• Plasmid: pRS413 pro 13Myc yeast ESC4
• Parent Plasmid: pRS413
• Expressed: 13Myc yeast ESC4
• Tag: Myc
• Species: Saccharomyces cerevisiae

Sequence of Insert

GCGGCCGCTCTAGAACTAGTGGATCCTCTCCAATAAGATATCCCAATAAGCTATTGAGAATTTCAAGCGCGTGAAATTCAAATTTATCATCAGAGAACGA
TGTGCGGGAACTCCAGGGCTTTTCTGTATTTTCTCATAGACATTATTTATCAGTAATTGCAGCTGTGTAACAATAAAATTGACGCCACTTTCTTCGTCTT
GTAGCTCCTCGTTAAGCTGCGAAATTAACCTGCTCGTATTCACAGTAACACACTTAACCACAGAATGTTCTTCGACATTAGAAATCTCTTGTATTTTTTG
TACATCCCAAAAGAATTCAGACATTTCTATTGAACCATTCCAGAAATGTTGTTCCAGAAGTTTATTCTCTTGAACCTTTACACTATTTCGTATTTTCTCG
GTTTTAGCCGCCCACTTTTCACGCGTTAACAGGGTAAGCTATATCAGATGTGACTTTTCTTTTTCACTCATCTAATGTTGTTTTTCGCGTCACATTTTTT
GCCAATAAATAAATACATCACAATGTTCTATATTGTATATTCAATGGAAGTCATTAGGTTATTGGGCAAGATTTCAAGGAGGTAGCATACTGGTAAATAT
ACGATccATGGCAGGTCGACGGATCCCCGGGTTAATTAACGGTGAACAAAAGCTAATCTCCGAGGAAGACTTGAACGGTGAACAAAAATTAATCTCAGAA
GAAGACTTGAACGGACTCGACGGTGAACAAAAGTTGATTTCTGAAGAAGATTTGAACGGTGAACAAAAGCTAATCTCCGAGGAAGACTTGAACGGTGAAC
AAAAATTAATCTCAGAAGAAGACTTGAACGGACTCGACGGTGAACAAAAGTTGATTTCTGAAGAAGATTTGAACGGTGAACAAAAGCTAATCTCCGAGGA
AGACTTGAACGGTGAACAAAAATTAATCTCAGAAGAAGACTTGAACGGACTCGACGGTGAACAAAAGTTGATTTCTGAAGAAGATTTGAACGGTGAACAA
AAGCTAATCTCCGAGGAAGACTTGAACGGTGAACAAAAATTAATCTCAGAAGAAGACTTGAACGGACTCGACGGTGAACAAAAGTTGATTTCTGAAGAAG
ATTTGAACGGTGAACAAAAGCTAATCTCCGAGGAAGACTTGAACGGTGAACAAAAATTAATCAATCACACCATGGGCACATCACTTCTGTTTGAGCAGCT
AAATTTTTTAATTTTAGTGGCAGCAGAGGCTGAGCTTCCCATCGCTCACTCTACTAGAAAGCTTTTGATGGATAATTCATGCAATAACTGTCAAATATAC
GAGCTTTATAACGAAAATTTGAAAGATGTCAAGACGGATAAAGATTGGTTCATGAACAAATTCGGCCCGCAAACAGTACATTTTGTCATTAGTAATACAA
TCAACTTTCCTTTTTATAAGATAGTTTATTTCGATCTTTTGATCCCGGTGGTATCACACACTTGGGTCCAGGATTCGGTCAAGACGAAGAGACATTTGAG
GACAAATATGTATTCACCTAACCCATTTCATTTGCTCCGAGACTGTCAAGTATATATTTCCAAAAGCTCGTTTAATAAGTGTGAATACATATTATACTCT
GATTTGCTTCATTTATTAGGAGGCACTTTAGTCAATTACATCTCAAATAGAACGACGCATGTGATTGTTCAAAGTCCCCAGGACCCGATCATAGCAACGG
TCAGTAAATTGACATTTGGCTCTTTTTCCTCTTCATCGACCAACAAGCACACCGAAAAGCCTTTGAGAGAGTGGAAATTTGTCTATCCAATATGGATATT
ATATCATTTCAAAATGGCGAAGCCCTTGAAGGGCGAGCTTGCTACTTTGTGTGAGCTTGATATGCAGGACACCAGTGAGGAACAGTTATTCGCAAAATGG
GAAGAGGTTATTGGCGATAAGCAGACATCATCATCGCAGCTTACTCTTCATCCAAACAAAACTTTATTCAAAAATCACCACTTTGCTATCTCACCAGATT
TAAACTTTTTCACACCTTTGTATTGGTTTTTGAAAGGATTTATCGAGGATTTGGACGGCAAAGTAACTCCGTTGAGTTTTTCTGACGATCTGAAGTCTGT
TTACCAAGCTTTTCCTGATATTGACTGTTATATCGGCCACTCTGCTAACTCACCGATACTAGAAAAAACCAAAAGCATTAAACCGGAAATTCATGTTGGG
AACGTAAGTTGGTTGTTTTATATGTTTGCGCTGCAAAAATTTACACCTGTATCGCAATGTAAACTTATTCACCAACCATTTCATGCGAAATTATTTACTT
CTAAGGAATTAACAGTCGCGTACACTAATTATTTCGGCTCCCAAAGGTTTTATATTCAGAGGTTAGTAGAAATATTAGGAGGACTTAGCACACCTGAACT
AACAAGAAAAAACACACACTTGATCACGAAGAGTACAATTGGCAAAAAATTCAAAGTCGCAAAAAAATGGTCACTGGACCCGCAAAATGCCATTATTGTG
ACAAATCATATGTGGTTAGAGCAATGTTATATGAATAATTCCAAGTTAAACCCCAAAGACAGTAGATTTCAAAATTTTAAATTGGATGATAACATGGGAT
GGAATATCGGCCAAATAGGAATGGATCATTCATCTCTCCCAACTCCAAAAAACCTATCGATGGTGACTTACGACACGCAATCCATATCAGAGAAGCCGCC
TCCCACAAATGATCAAATGGATCAAATTAACGATAACACCAATGTATTATCAAAGAAAGATGGTACGCCCATTAGTAGTTTTGAGAACTCAATTGATGAA
AAGATTGACAAATTACAAAAAATATCAGGAGAGGTGGCTGTAACCCATTCTGGAGATTTAGAACGGAGTTTTGTTTCCAGACCTTCAAGGGCAAGTTTTC
CAGTAGTTGACAGTAAAAAATCTAATTTACAAAAGAAAGACTCAAACTCAGATATTAGTATGGAAACAGAGGTTTTCTGTGAAGGACATGAAAAAAGAGA
AGAAAAGGAGTTTACGAAACCTATTACGGAGTATGATGCACCTAAGAAACAAGAAATAAGGGAACAGTCACGCAAGAAGAATGACATAGATTATAAAAAA
GAAGAGGAAGAAACGGAGCTGCAAGTTCAGCTAGGACAACGAACTAAACGAGAGATCAAAACTTCGAAGAAAAATGAAAAGGAGAAAGAAACCAATGAAT
GTCACATTGAAGTAGATCAAATGACGAATGAAAAGCAAGGGGAGGAAAGTACAGGAAAACTGATTTCTACGGAAGACGTCACTTCTAAAAAAGATACAGA
TAAATTCTCGCATTTGTTTGAAGGATTATCTGATAATGATGATCACATTAATGACGAAAAACCGGCAGTTAATTCCAAATATACCACACCCAAGACGTCT
CAAAATATTACGAGTGGCGTTGATACTCCAACCACCGCGCAAACGCAAGTTTTTATGCCATCAAGTGGCAATTCAAGGTTAGCAAAGACACAAGCTGCGA
AGAGATTGCATACAGATATTGAATCATTAAATGAATTTCAGAAAAACTTTAAAAGGAAGAGAATTGATAGCGAGGAGATTTCATTGTCCCAAGATGTTGA
AAGGTCAAATAATAACAAAGAACTGGCTACTAAAGCGGAAAAGATATTAGCTAGATTTAATGAACTACCAAATTATGACCTAAAAGCGGTTTGCACCGGT
TGTTTCCATGATGGATTTAATGAGGTTGACATCGAAATATTGAACCAACTAGGTATAAAAATTTTTGACAATATTAAAGAAACGGATAAATTGAATTGTA
TTTTTGCTCCAAAGATTTTGAGAACCGAGAAATTTTTAAAAAGCTTAAGTTTTGAACCGTTAAAATTCGCATTGAAACCTGAATTTATTATTGATTTACT
GAAACAAATTCATTCTAAAAAAGATAAACTTTCTCAAATAAATATAAACTTGTTTGATTATGAGATAAATGGAATTAATGAGAGTATAATATCAAAAACC
AAACTACCTACTAAGGTGTTCGAAAGAGCGAATATTAGATGCATTAACTTAGTCAATGATATACCGGGTGGTGTAGATACAATAGGCTCTGTTCTAAAGG
CACATGGTATCGAAAAAATCAACGTTTTACGTAGTAAGAAATGCACATTTGAAGACATAATCCCAAATGACGTTTCTAAGCAGGAAAACGGAGGCATTTT
TAAATACGTACTTATCGTCACCAAAGCTTCACAGGTTAAAAAATTTACCAAACTGATTAATGATCGTGATAAAAACGAAACAATTTTGATTGTTGAATGG
AATTGGTGTGTTGAATCAATTTTTCATCTCAATGTTGATTTTACATCAAAGAAAAATGTTTTGTATCAAAAGAAAAATAACTAAGTCGACCTCGAG

Amino Acid Sequence

MAGRRIPGLINGEQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISEEDL
NGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISEEDLNGEQKLINHTMGTSLLFEQLNF
LILVAAEAELPIAHSTRKLLMDNSCNNCQIYELYNENLKDVKTDKDWFMNKFGPQTVHFVISNTINFPFYKIVYFDLLIPVVSHTWVQDSVKTKRHLRTN
MYSPNPFHLLRDCQVYISKSSFNKCEYILYSDLLHLLGGTLVNYISNRTTHVIVQSPQDPIIATVSKLTFGSFSSSSTNKHTEKPLREWKFVYPIWILYH
FKMAKPLKGELATLCELDMQDTSEEQLFAKWEEVIGDKQTSSSQLTLHPNKTLFKNHHFAISPDLNFFTPLYWFLKGFIEDLDGKVTPLSFSDDLKSVYQ
AFPDIDCYIGHSANSPILEKTKSIKPEIHVGNVSWLFYMFALQKFTPVSQCKLIHQPFHAKLFTSKELTVAYTNYFGSQRFYIQRLVEILGGLSTPELTR
KNTHLITKSTIGKKFKVAKKWSLDPQNAIIVTNHMWLEQCYMNNSKLNPKDSRFQNFKLDDNMGWNIGQIGMDHSSLPTPKNLSMVTYDTQSISEKPPPT
NDQMDQINDNTNVLSKKDGTPISSFENSIDEKIDKLQKISGEVAVTHSGDLERSFVSRPSRASFPVVDSKKSNLQKKDSNSDISMETEVFCEGHEKREEK
EFTKPITEYDAPKKQEIREQSRKKNDIDYKKEEEETELQVQLGQRTKREIKTSKKNEKEKETNECHIEVDQMTNEKQGEESTGKLISTEDVTSKKDTDKF
SHLFEGLSDNDDHINDEKPAVNSKYTTPKTSQNITSGVDTPTTAQTQVFMPSSGNSRLAKTQAAKRLHTDIESLNEFQKNFKRKRIDSEEISLSQDVERS
NNNKELATKAEKILARFNELPNYDLKAVCTGCFHDGFNEVDIEILNQLGIKIFDNIKETDKLNCIFAPKILRTEKFLKSLSFEPLKFALKPEFIIDLLKQ
IHSKKDKLSQININLFDYEINGINESIISKTKLPTKVFERANIRCINLVNDIPGGVDTIGSVLKAHGIEKINVLRSKKCTFEDIIPNDVSKQENGGIFKY
VLIVTKASQVKKFTKLINDRDKNETILIVEWNWCVESIFHLNVDFTSKKNVLYQKKNN*

• Vector Type: Yeast
• Antibiotic: Amp
• Full Snapgene Map: pRS413 pro 13Myc yeast ESC4132.22 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU58601
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Low Temp: No