cDNA Clone KIAA1390

• Plasmid: pGEX6P1 KIAA1390 Y114A
• Parent Plasmid: pGEX6P1
• Expressed: GST KIAA1390 Y114A
• Tag: GST

Sequence of Insert

AGATCTATGGAATACCATCAGCCTGAGGATCCAGCCCCTGGTAAGGCCGGGACTGCAGAAGCAGTCATCCCTGAAAACCATGAGGTTCTGGCAGGCCCAGATGAGCACCCTCAGGACACAGATGCAAGAGATGCTGATGGGGAGGCTAGAGAACGGGAGCCAGCAGACCAAGCTTTGCTGCCTAGCCAGTGTGGGGACAACCTTGAGTCCCCTCTGCCTGAAGCTAGCTCAGCTCCACCGGGGCCAACCCTTGGGACACTGCCTGAAGTAGAGACAATAAGGGCATGCTCCATGCCCCAGGAGCTTCCTCAGTCCCCCAGGACCCGACAGCCTGAGCCAGATTTCGCCTGTGTCAAGTGGATCCCTTGGAAAGGAGAACAGACACCCATCATCACCCAGAGCACTAACGGCCCTTGCCCTCTCCTTGCCATCATGAACATCCTCTTTCTTCAGTGGAAGGTGAAGCTCCCCCCGCAGAAGGAAGTGATCACATCGGATGAGCTCATGGCCCATCTTGGAAACTGCCTCCTGTCCATCAAGCCCCAGGAGAAGTCAGAGGGACTTCAGCTTAATTTTCAGCAGAATGTGGATGATGCAATGACAGTGCTGCCTAAACTGGCCACAGGTCTGGATGTCAATGTGCGATTCACAGGCGTCTCTGATTTTGAGTATACACCCGAGTGCAGTGTCTTTGACCTGCTAGGCATACCTCTGTACCATGGCTGGCTTGTTGATCCACAGAGTCCTGAGGCTGTGCGTGCAGTTGGGAAACTGAGTTACAACCAGCTGGTGGAGAGGATCATCACCTGCAAACACTCCAGTGACACCAACCTCGTGACAGAAGGCCTGATTGCAGAGCAGTTCCTGGAGACCACCGCGGCCCAGCTGACCTACCACGGACTGTGTGAGCTGACAGCAGCTGCTAAGGAGGGTGAACTTAGCGTCTTTTTCCGAAACAACCACTTTAGCACCATGACTAAGCATAAGAGTCACTTATACCTACTGGTCACTGACCAGGGCTTTCTACAGGAGGAGCAAGTCGTATGGGAGAGCCTGCACAATGTGGATGGAGACAGCTGCTTTTGTGACTCTGACTTTCACCTGAGTCATTCCCTGGGCAAGGGGCCTGGAGCAGAAGGTGGGAGTGGCTCCCCAGAAAAGCAGCTGCAGGTAGACCAGGACTACCTGATTGCTCTGTCCCTGCAGCAGCAACAGCCACGAGGCCCGCTGGGGCTTACCGACTTGGAGCTGGCCCAGCAGCTTCAGCAAGAGGAGTATCAACAGCAGCAGGCAGCGCAGCCAGTGCGGATGCGGACGCGGGTCCTGTCACTGCAGGGGAGAGGAGCCACATCTGGACGCCCAGCCGGGGAGCGTCGGCAGAGGCCGAAGCACGAGTCAGACTGCATTCTGCTGTAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMEYHQPEDPAPGKAGTAEAVIPENHEVLAGPDEHPQDTDARDADGEAREREPADQALLPSQCGDNLESPLPEASSAPPGPTLGTLPEVETIRACSMPQELPQSPRTRQPEPDFACVKWIPWKGEQTPIITQSTNGPCPLLAIMNILFLQWKVKLPPQKEVITSDELMAHLGNCLLSIKPQEKSEGLQLNFQQNVDDAMTVLPKLATGLDVNVRFTGVSDFEYTPECSVFDLLGIPLYHGWLVDPQSPEAVRAVGKLSYNQLVERIITCKHSSDTNLVTEGLIAEQFLETTAAQLTYHGLCELTAAAKEGELSVFFRNNHFSTMTKHKSHLYLLVTDQGFLQEEQVVWESLHNVDGDSCFCDSDFHLSHSLGKGPGAEGGSGSPEKQLQVDQDYLIALSLQQQQPRGPLGLTDLELAQQLQQEEYQQQQAAQPVRMRTRVLSLQGRGATSGRPAGERRQRPKHESDCILL*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU59114
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: please mutate Tyr114 to Ala in the backbone of 49563
• Low Temp: No