cDNA Clone LCK (mouse)

• Plasmid: pFastbacDual 6His-mouse LCK G2-P509(end)/6His-human RAS T2-S189(end)
• Parent Plasmid: pFastbacDual
• Expressed: 6His-mouse LCK G2-P509(end)
• Genbank: X03533.1*
• Tag: 6His
• Species: Mouse

Sequence of Insert

GAATTCGAAACCATGCATCATCACCATCACCATGGCTGTGGCTGCAGCTCACACCCTGAAGATGACTGGATGGAGAACATTGACGTGTGTGAAAACTGCCACTATCCCATAGTCCCACTGGACAGCAAGATCTCGCTGCCCATCCGGAATGGCTCTGAAGTGCGGGACCCACTGGTCACCTATGAGGGATCTCTCCCACCAGCATCCCCGCTGCAAGACAACCTGGTTATCGCCCTGCACAGTTATGAGCCCTCCCATGATGGAGACTTGGGCTTTGAGAAGGGTGAACAGCTCCGAATCCTGGAGCAGAGCGGTGAGTGGTGGAAGGCTCAGTCCCTGACGACTGGCCAAGAAGGCTTCATTCCCTTCAACTTCGTGGCGAAAGCAAACAGCCTGGAGCCTGAACCTTGGTTCTTCAAGAATCTGAGCCGTAAGGACGCCGAGCGGCAGCTTTTGGCGCCCGGGAACACGCATGGATCCTTCCTGATCCGGGAAAGCGAAAGCACTGCGGGGTCCTTTTCCCTGTCGGTCAGAGACTTCGACCAGAACCAGGGAGAAGTGGTGAAACATTACAAGATCCGTAACCTAGACAACGGTGGCTTCTACATCTCCCCTCGTATCACTTTTCCCGGATTGCACGATCTAGTCCGCCATTACACCAACGCCTCTGATGGGCTGTGCACAAAGTTGAGCCGTCCTTGCCAGACCCAGAAGCCCCAGAAACCATGGTGGGAGGACGAATGGGAAGTTCCCAGGGAAACACTGAAGTTGGTGGAGCGGCTGGGAGCTGGCCAGTTCGGGGAAGTGTGGATGGGGTACTACAACGGACACACGAAGGTGGCGGTGAAGAGTCTGAAACAAGGGAGCATGTCCCCCGACGCCTTCCTGGCTGAGGCTAACCTCATGAAGCAGCTGCAGCACCCGCGGCTAGTCCGGCTTTATGCAGTGGTCACCCAGGAACCCATCTACATCATCACGGAATACATGGAGAACGGGAGCCTAGTAGATTTTCTCAAGACTCCCTCGGGCATCAAGTTGAATGTCAACAAACTTTTGGACATGGCAGCCCAGATTGCAGAGGGCATGGCGTTCATCGAAGAACAGAATTACATCCATCGGGACCTGCGCGCCGCCAACATCCTGGTGTCTGACACGCTGAGCTGCAAGATTGCAGACTTTGGCCTGGCGCGCCTCATTGAGGACAATGAGTACACGGCCCGGGAGGGGGCCAAATTTCCCATTAAGTGGACAGCACCAGAAGCCATTAACTATGGGACCTTCACCATCAAGTCAGACGTGTGGTCCTTCGGGATCTTGCTTACAGAGATCGTCACCCACGGTCGAATCCCTTACCCAGGAATGACCAACCCTGAAGTCATTCAGAACCTGGAGAGAGGCTACCGCATGGTGAGACCTGACAACTGTCCGGAAGAGCTGTACCACCTCATGATGCTGTGCTGGAAGGAGCGCCCAGAGGACCGGCCCACGTTTGACTACCTTCGGAGTGTTCTGGATGACTTCTTCACAGCCACAGAGGGCCAGTTCCAGCCCCAGCCTGTCGACCTCTAGGCGGCCGC

Amino Acid Sequence

MHHHHHHGCGCSSHPEDDWMENIDVCENCHYPIVPLDSKISLPIRNGSEVRDPLVTYEGSLPPASPLQDNLVIALHSYEPSHDGDLGFEKGEQLRILEQS
GEWWKAQSLTTGQEGFIPFNFVAKANSLEPEPWFFKNLSRKDAERQLLAPGNTHGSFLIRESESTAGSFSLSVRDFDQNQGEVVKHYKIRNLDNGGFYIS
PRITFPGLHDLVRHYTNASDGLCTKLSRPCQTQKPQKPWWEDEWEVPRETLKLVERLGAGQFGEVWMGYYNGHTKVAVKSLKQGSMSPDAFLAEANLMKQ
LQHPRLVRLYAVVTQEPIYIITEYMENGSLVDFLKTPSGIKLNVNKLLDMAAQIAEGMAFIEEQNYIHRDLRAANILVSDTLSCKIADFGLARLIEDNEY
TAREGAKFPIKWTAPEAINYGTFTIKSDVWSFGILLTEIVTHGRIPYPGMTNPEVIQNLERGYRMVRPDNCPEELYHLMMLCWKERPEDRPTFDYLRSVL
DDFFTATEGQFQPQPVDL*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU3140
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: * Residue changes compared with X03533.1: V4G, N8H, Y505F. This clone also has an additional 3 residues at the C-terminus of LCK (VDL) before the stop codon.
• Low Temp: No