cDNA Clone Esc4 (yeast)

• Plasmid: pRS415 pro 13Myc yeast ESC4 W98F W208F W347F W448F
• Parent Plasmid: pRS415
• Expressed: 13Myc yeast ESC4 W98F W208F W347F W448F
• Tag: Myc
• Species: Saccharomyces cerevisiae

Amino Acid Sequence

MAGRRIPGLINGEQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISEEDL
NGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISEEDLNGEQKLINHTMGTSLLFEQLNF
LILVAAEAELPIAHSTRKLLMDNSCNNCQIYELYNENLKDVKTDKDWFMNKFGPQTVHFVISNTINFPFYKIVYFDLLIPVVSHTFVQDSVKTKRHLRTN
MYSPNPFHLLRDCQVYISKSSFNKCEYILYSDLLHLLGGTLVNYISNRTTHVIVQSPQDPIIATVSKLTFGSFSSSSTNKHTEKPLREWKFVYPIFILYH
FKMAKPLKGELATLCELDMQDTSEEQLFAKWEEVIGDKQTSSSQLTLHPNKTLFKNHHFAISPDLNFFTPLYWFLKGFIEDLDGKVTPLSFSDDLKSVYQ
AFPDIDCYIGHSANSPILEKTKSIKPEIHVGNVSFLFYMFALQKFTPVSQCKLIHQPFHAKLFTSKELTVAYTNYFGSQRFYIQRLVEILGGLSTPELTR
KNTHLITKSTIGKKFKVAKKWSLDPQNAIIVTNHMFLEQCYMNNSKLNPKDSRFQNFKLDDNMGWNIGQIGMDHSSLPTPKNLSMVTYDTQSISEKPPPT
NDQMDQINDNTNVLSKKDGTPISSFENSIDEKIDKLQKISGEVAVTHSGDLERSFVSRPSRASFPVVDSKKSNLQKKDSNSDISMETEVFCEGHEKREEK
EFTKPITEYDAPKKQEIREQSRKKNDIDYKKEEEETELQVQLGQRTKREIKTSKKNEKEKETNECHIEVDQMTNEKQGEESTGKLISTEDVTSKKDTDKF
SHLFEGLSDNDDHINDEKPAVNSKYTTPKTSQNITSGVDTPTTAQTQVFMPSSGNSRLAKTQAAKRLHTDIESLNEFQKNFKRKRIDSEEISLSQDVERS
NNNKELATKAEKILARFNELPNYDLKAVCTGCFHDGFNEVDIEILNQLGIKIFDNIKETDKLNCIFAPKILRTEKFLKSLSFEPLKFALKPEFIIDLLKQ
IHSKKDKLSQININLFDYEINGINESIISKTKLPTKVFERANIRCINLVNDIPGGVDTIGSVLKAHGIEKINVLRSKKCTFEDIIPNDVSKQENGGIFKY
VLIVTKASQVKKFTKLINDRDKNETILIVEWNWCVESIFHLNVDFTSKKNVLYQKKNN*

• Vector Type: Yeast
• Antibiotic: Amp
• DU Number: DU58662
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Yeast non-intergrating expression plasmid. Sequence differences in ESC4 compared with reference CDS: a4g (S2G) (resulting from generation of Nco1 site) t3006g (silent in S1002).
• Low Temp: No