cDNA Clone POLE1 (Mouse)

• Plasmid: pGEX6P1 POLE1 M1-R222 mouse
• Parent Plasmid: pGEX6P1
• Expressed: GST POLE1 M1-R222 mouse
• Genbank: NP_035262.2
• Tag: GST
• Species: Mouse

Sequence of Insert

GGATCCATGGTCCTGAGGAACAGTGGACGGAGGCACCCCGAGCCGGGCGCGGATGGCGAAGGCAGCCGGGATGATGGTCCCTCTTCCTCAGTCTCAGCACTCAAGCGTCTGGAACGGAGCCAGTGGACAGACAAGATGGACTTACGGTTTGGTTTCGAAAGGCTGAAAGAGCCTGGAGAAAGGACTGGCTGGCTGATCAACATGCACCCTACTGAGATCTTAGATGAAGACAAACGCTTAGTCAGCGCGGTGGATTACTACTTCATTCAGGATGATGGAAGCAGATTTAAGGTGGCCTTGCCCTATATGCCGTATTTCTACATTGCAGCGAGAAAGGGTTGTGATCGAGAAGTTTCATCTTTTCTATCCAAGAAGTTTCAGGGAAAAATTGCAAAGTTAGAGAATGTGCCCAAAGAAGATCTGGACTTGCCAAATCACTTGGTGGGCTTGAAGCGGAGTTACATCAAGCTGTCCTTCCACACTGTGGAGGACCTTGTCAAAGTGAGAAAGGAGATCTCTCCTGCTGTGAAGAAGAACCGAGAGCAGGACCATGCTAGTGATGAGTATACAACAATGCTCTCCAGTATTCTGCAAGGTGGCAGTGTAATTACTGATGAGGACGAAACCTCTAAGAAGATAGCTGACCAATTGGACAACATAGTGGACATGCGATGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMVLRNSGRRHPEPGADGEGSRDDGPSSSVSALKRLERSQWTDKMDLRFGFERLKEPGERTGWLINMHPTEILDEDKRLVSAVDYYFIQDDGSRFKVALPYMPYFYIAARKGCDREVSSFLSKKFQGKIAKLENVPKEDLDLPNHLVGLKRSYIKLSFHTVEDLVKVRKEISPAVKKNREQDHASDEYTTMLSSILQGGSVITDEDETSKKIADQLDNIVDMR*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU27959
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Silent mutation Q88
• Low Temp: No