cDNA Clone aPINK1

• Synonyms: anti-PINK1 nanobody, PINK1
• Plasmid: pMERI aPINK1 ph wt nano c-HIS
• Parent Plasmid: pMERI
• Expressed: pelB-MBP-3C-aPINK1 ph wt nano-6HIS
• Tag: 6His
• Tag: MBP
• Tag: 6HN
• Species: Human

Sequence of Insert

ggatcccaggtgcagctggtggaaagcggcggcggcctggtgcaggcgggcggcagcctgaaactgagctgcaccaccagcggtcgcccgtttagcacctttgatctggcgtggtttcgccaggcgccgggcaaagaacgcgaatttgtgagcggcctgcgccgcggcggcagcaccttttatgcggatagcgtgagcggacgctttaccattagcggcggcagcaccaaaaacaccctgtatctgcagatgaacagcctgaaaccggaagataccgcgatttattattgcgcgcgcagcattaccggctatggcctggtggatagcgcgagccattataccatttggggccagggcacccaggtgaccgtgagcagccatcatcatcatcatcatgaaccggaagcgtgagcggccgc

Amino Acid Sequence

MKYLLPTAAAGLLLLAAQPAMAKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAE
ITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDI
KDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKE
FLENYLLTDEGLEAVNKDKPLGAVALKSYEEELVKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNNNNNN
NNLGDDDDKVPEFLEVLFQGPLGSQVQLVESGGGLVQAGGSLKLSCTTSGRPFSTFDLAWFRQAPGKEREFVSGLRRGGSTFYADSVSGRFTISGGSTKN
TLYLQMNSLKPEDTAIYYCARSITGYGLVDSASHYTIWGQGTQVTVSSHHHHHHEPEA*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pMERI aPINK1 ph wt nano c-HIS124.53 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU60326
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Anti-PINK1 nanobody for use in degron generation.
• Low Temp: No