cDNA Clone aPINK1

• Synonyms: anti-PINK1 nanobody
• Plasmid: pMERI aPINK1 nano1 c-HIS
• Parent Plasmid: pMERI
• Expressed: pelB-MBP-3C-aPINK1 nano1-6HIS
• Tag: 6His
• Tag: MBP
• Species: Human

Sequence of Insert

ggatcccgcgtgcagctggtggaaagcggcggcggcctggtgcaggcgggcggcagcctgaaactgagctgcaccaccagcggcctgccgtttagctgctttgatctggcgtggtttcgccaggcgccgggcaaagaacgcgaatttgtgagcggcctgcgccgcggcggcagcaccttttatgcggatagcgtgagcggtcgctttaccattagcggcggcagcacctgcctgaccctgtatctgcagatgaacagcctgaaaccggaagataccgcgatttattattgcgcgcgcagcattaccggctatggcctggtggatagcgcgagccattataccatttggggccagggcacccaggtgcaggtgcagagccatcatcatcatcatcatgaaccggaagcgtgagcggccgc

Amino Acid Sequence

MKYLLPTAAAGLLLLAAQPAMAKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAE
ITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDI
KDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKE
FLENYLLTDEGLEAVNKDKPLGAVALKSYEEELVKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNNNNNN
NNLGDDDDKVPEFLEVLFQGPLGSRVQLVESGGGLVQAGGSLKLSCTTSGLPFSCFDLAWFRQAPGKEREFVSGLRRGGSTFYADSVSGRFTISGGSTCL
TLYLQMNSLKPEDTAIYYCARSITGYGLVDSASHYTIWGQGTQVQVQSHHHHHHEPEA*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pMERI aPINK1 nano1 c-HIS22.31 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU60327
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Anti-PINK1 nanobody for use in degron generation.
• Low Temp: No