cDNA Clone Mcm7 (yeast)

• Plasmid: pSS17 MCM7 3TEV after L47 5 Flag
• Parent Plasmid: pSS17
• Expressed: MCM7 5 Flag
• Species: Yeast

Sequence of Insert

GTCGACTATAATAATCTTTTTAATGAAATCACCGATTTCTTGGTGACTTTCAAGCAGGATACGTTGTCTTCCGATGCAACACGAAATGAGAACGAAGATGAAAATTTGGGTGCTTCTGAGAACTTGTACTTCCAAGGAGCAACCGCTTCCGAAAATTTATATTTCCAGGGCTCCGCCACCGGAGCTGAAAACTTATACTTTCAGGGTGCTGGTGATGCTGAGAATATCGAACAACATCTATTGGAAAAGGGGCCCAAGTACATGGCGATGCTACAGAAAGTGGCTAATAGGGAACTGAATTCCGTGATTATTGATTTAGATGACATTCTACAGTATCAAAACGAAAAGTTTCTCCAGGGCACACAGGCAGACGACCTTGTGTCTGCCATTCAGCAAAACGCTAATCACTTCACTGAATTATTTTGCCGTGCCATTGACAACAACATGCCGCTACCAACAAAAGAAATTGACTACAAGGACGACGTTCTTGATGTTATTTTAAACCAAAGGAGACTGAGGAATGAGAGAATGCTCTCGGACAGGACCAATGAGATCCGAAGCGAAAACCTCATGGATACTACGATGGATCCACCCTCTTCCATGAATGATGCGTTAAGAGAAGTTGTCGAGGATGAAACTGAGTTATTTCCTCCTAATTTAACTAGACGCTATTTCCTTTATTTTAAGCCTCTATCGCAAAATTGTGCTCGTCGTTACAGGAAGAAAGCAATTAGTTCTAAACCATTATCTGTTAGACAGATTAAAGGTGATTTCCTAGGCCAATTGATTACCGTCAGAGGTATTATCACCAGAGTTTCTGATGTCAAACCAGCTGTGGAAGTTATCGCATATACCTGCGATCAATGTGGGTACGAAGTTTTCCAAGAGGTCAACTCTCGTACTTTTACCCCGTTGTCAGAATGTACTTCAGAAGAATGTTCCCAAAATCAAACAAAAGGTCAATTGTTTATGAGTACACGTGCTTCCAAATTTAGTGCCTTTCAGGAATGTAAAATTCAGGAGTTATCACAACAAGTGCCAGTGGGTCATATTCCTAGGTCGCTAAATATCCACGTTAACGGGACGCTGGTAAGATCTTTATCGCCTGGTGATATCGTCGATGTAACTGGTATATTCTTGCCAGCGCCCTACACCGGTTTCAAAGCTTTGAAAGCTGGCTTACTGACAGAAACTTATTTAGAGGCACAGTTTGTCCGTCAACACAAGAAGAAATTTGCGTCTTTCAGTCTGACTTCTGATGTAGAAGAGAGAGTTATGGAATTAATCACCTCTGGTGATGTATATAATAGGCTGGCAAAATCTATTGCACCGGAAATCTACGGTAACTTGGACGTTAAGAAAGCGTTGCTGCTATTACTTGTCGGAGGTGTTGATAAAAGGGTAGGTGACGGTATGAAAATCAGAGGTGATATCAATGTTTGTCTGATGGGTGATCCCGGTGTTGCCAAATCTCAACTGCTGAAGGCCATTTGCAAAATATCACCTCGAGGAGTGTATACCACTGGTAAGGGTTCCTCAGGCGTTGGTCTGACCGCTGCCGTCATGAAAGATCCTGTCACGGATGAAATGATTCTAGAAGGTGGTGCCTTAGTACTCGCTGATAACGGTATTTGTTGTATCGATGAATTTGATAAGATGGATGAAAGTGACAGAACGGCAATCCATGAAGTTATGGAACAACAAACAATTTCGATATCCAAGGCGGGTATCAATACAACTTTGAACGCCAGAACCTCAATCTTAGCGGCAGCAAATCCGTTGTATGGTAGATATAATCCTAGATTATCACCTCTGGACAATATAAATCTACCAGCTGCTTTACTATCCAGATTTGATATTCTCTTCTTAATGTTAGATATACCAAGTAGAGATGACGACGAAAAATTAGCCGAGCACGTTACATATGTGCATATGCATAATAAACAACCGGATTTGGACTTCACTCCAGTAGAACCCTCTAAAATGAGAGAGTACATTGCCTATGCTAAGACAAAGAGGCCAGTGATGAGTGAAGCTGTAAACGATTATGTGGTGCAAGCTTACATCAGATTAAGACAAGACTCCAAGAGAGAAATGGATTCCAAATTTTCCTTTGGTCAAGCCACTCCGAGAACTTTACTAGGTATTATAAGATTATCTCAAGCGCTAGCAAAGTTAAGGTTGGCTGATATGGTGGATATAGATGATGTGGAGGAAGCCTTGAGGTTGGTCCGAGTCTCCAAGGAATCATTGTATCAAGAAACCAACAAATCTAAAGAAGATGAAAGCCCCACAACGAAAATCTTTACGATCATCAAGAAAATGTTACAAGAAACTGGTAAAAACACGCTATCATATGAAAATATTGTGAAAACTGTAAGACTAAGAGGGTTTACCATGCTACAATTAAGCAACTGTATCCAAGAATATTCTTACTTAAACGTCTGGCATTTAATTAATGAGGGTAACACTTTGAAATTCGTGGATGACGGTACCATGGACACAGACCAGGAAGACTCACTAGTGAGCACGCCTAAACTCGCACCACAAACGACCGCTTCCGCTAACGCGAGCGCCCAAGATTCTGATATCGATCTACAAGACGCTTCCGGTTCTGCTGCTAGTGGTCATATGGACTACAAGGACGACGATGACAAGGATTACAAAGATGACGACGATAAGCTTATGGACTACAAGGACGACGATGACAAGGATTACAAAGATGACGACGATAAGCTTATGGACTACAAGGACGACGATGACAAGCATATGTAAGTCGAC

Amino Acid Sequence

YNNLFNEITDFLVTFKQDTLSSDATRNENEDENLGASENLYFQGATASENLYFQGSATGAENLYFQGAGDAENIEQHLLEKGPKYMAMLQKVANRELNSVIIDLDDILQYQNEKFLQGTQADDLVSAIQQNANHFTELFCRAIDNNMPLPTKEIDYKDDVLDVILNQRRLRNERMLSDRTNEIRSENLMDTTMDPPSSMNDALREVVEDETELFPPNLTRRYFLYFKPLSQNCARRYRKKAISSKPLSVRQIKGDFLGQLITVRGIITRVSDVKPAVEVIAYTCDQCGYEVFQEVNSRTFTPLSECTSEECSQNQTKGQLFMSTRASKFSAFQECKIQELSQQVPVGHIPRSLNIHVNGTLVRSLSPGDIVDVTGIFLPAPYTGFKALKAGLLTETYLEAQFVRQHKKKFASFSLTSDVEERVMELITSGDVYNRLAKSIAPEIYGNLDVKKALLLLLVGGVDKRVGDGMKIRGDINVCLMGDPGVAKSQLLKAICKISPRGVYTTGKGSSGVGLTAAVMKDPVTDEMILEGGALVLADNGICCIDEFDKMDESDRTAIHEVMEQQTISISKAGINTTLNARTSILAAANPLYGRYNPRLSPLDNINLPAALLSRFDILFLMLDIPSRDDDEKLAEHVTYVHMHNKQPDLDFTPVEPSKMREYIAYAKTKRPVMSEAVNDYVVQAYIRLRQDSKREMDSKFSFGQATPRTLLGIIRLSQALAKLRLADMVDIDDVEEALRLVRVSKESLYQETNKSKEDESPTTKIFTIIKKMLQETGKNTLSYENIVKTVRLRGFTMLQLSNCIQEYSYLNVWHLINEGNTLKFVDDGTMDTDQEDSLVSTPKLAPQTTASANASAQDSDIDLQDASGSAASGHMDYKDDDDKDYKDDDDKLMDYKDDDDKDYKDDDDKLMDYKDDDDKHM*

• Vector Type: Yeast
• Antibiotic: Amp
• DU Number: DU62093
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Low Temp: No