cDNA Clone POLA-1 (C.elegans)

• Plasmid: pGEX6P1 POLA-1 M1-V222
• Parent Plasmid: pGEX6P1
• Expressed: GST POLA-1 M1-V222
• Genbank: NP_001255109.1
• Tag: GST
• Species: Caenorhabditis elegans

Sequence of Insert

GGATCCATGTCCGACTCGGAAAACCATGCAGAATCGGATGTTAGACGCTCGTCTCGCCGCTGTGGAGTCAGTCAAAAGAAGTCGAAGGAGCTGTCTGCAATTGAGCAGCTGAAATTGGCGCGATCAACAGGAAAAGCCTATCGCCCAGAGCTCGACGTGGAGGACGTCTACGACACGGTTGATGATGATGAGTACGAGAAAATCGTTGCAAAACGACAAAAGGACAATTTTATTGTCGATGATGATGGAATGGGCTACGTAGACAACGGTGTAGACTTTGAAGATGACTACGAAGACGATTATGCGCCCAGTTCTTCGAAAAATTCAAATTCCTCGAAAAAAGGCGAGAAAAAGCCGAAAAAAGACAAGAAAAAGGGAGGACTTCATGCATTCCTCTCCACAACCTCCACCAAGAAGCCGATCGACGAGTCTGTGGTGAAAGCCGAGTTTGACGACGATGAGGAGGTGAAAAATATGCTGGAGCAGCTGGGAAGCAGCGATGAGGAGGCCGAGGAGAAGCCGAAGGCGACCCCAGCGAATCCGTTCAAGCGGGGACGAAAATTCGACTCACCGAGCGGCTCGGATTCACTGGCTCCACGGCCGATTGCTAAGAAGGCCAAGAGAGAGGCGACCGGGCTCGCCGAGCACAAATTCCAGCGGCATGTGAAGGTTTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMSDSENHAESDVRRSSRRCGVSQKKSKELSAIEQLKLARSTGKAYRPELDVEDVYDTVDDDEYEKIVAKRQKDNFIVDDDGMGYVDNGVDFEDDYEDDYAPSSSKNSNSSKKGEKKPKKDKKKGGLHAFLSTTSTKKPIDESVVKAEFDDDEEVKNMLEQLGSSDEEAEEKPKATPANPFKRGRKFDSPSGSDSLAPRPIAKKAKREATGLAEHKFQRHVKV*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU62097
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Low Temp: No