cDNA Clone POLE-2 (C.elegans)

• Plasmid: pGEX6P1 POLE-2 M1-V222 C.elegans
• Expressed: GST POLE-2 M1-V222 C.elegans
• Genbank: NP_501489.3
• Tag: GST
• Species: Caenorhabditis elegans

Sequence of Insert

ATGGACACAACTGCCGAGGAGAATGCGCTGCGCCGAGAAATTGTCAAAACCTTTCAGATACACGCATTTGAGCTCAAAAAAGAAGCGGTCAAATTATGTGTGAAGCTATTCCTAGACCATGACAAAGAAACTCGAAAGAAATGGACGAATAAAATGATTGAATTACTCAAAAAGCAGACACTGCAATCATCCCTAATCAGTGAAGAACTCATCAGAGATACATTCCGTCAATGTAAATCAAAAGGAACCCAAGATGCGGGAAAACTGCTCAATGTATTCGACGCATTCTCCCTCCAGCCGTATGATTTTGATGCGGATCTTCGGAAAATGGTTCTAAGAAAAGAAAAAACTAGTCTGGCTGCTGATTCGTCGTCATTCTCACATGCTGCACGACAACGATTTTTTATTGTTAAGCAGCGTGCAATGCGATGTGCTTCACTGAAAAACTTCAAATTCACCACATGCGAAATTTTGGGAAGTTCCACAAAACATCTTCAATCAGTTGTAGTTCTCGGTATGCTTACTCAGCAAAAGGCCGACTGCTATCATATCGAAGATCTAAGCGGTTCAGTTGAAGTAGAATTTAAAGAAGACACGAAATTTCATCATGCGTTATTCCATGAGCATTCAATTGCAATTTTTGAAGGAACTTTTGAGAATAGTGTTTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMDTTAEENALRREIVKTFQIHAFELKKEAVKLCVKLFLDHDKETRKKWTNKMIELLKKQTLQSSLISEELIRDTFRQCKSKGTQDAGKLLNVFDAFSLQPYDFDADLRKMVLRKEKTSLAADSSSFSHAARQRFFIVKQRAMRCASLKNFKFTTCEILGSSTKHLQSVVVLGMLTQQKADCYHIEDLSGSVEVEFKEDTKFHHALFHEHSIAIFEGTFENSV*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU62147
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Silent mutation A86
• Low Temp: No