cDNA Clone POLE-2 (C.elegans)

• Plasmid: pMEX3C POLE-2 M1-V222 C.elegans
• Parent Plasmid: pMEX3C
• Expressed: MBP POLE-2 M1-V222 C.elegans
• Genbank: NP_501489.3
• Tag: MBP
• Species: Caenorhabditis elegans

Sequence of Insert

ATGGACACAACTGCCGAGGAGAATGCGCTGCGCCGAGAAATTGTCAAAACCTTTCAGATACACGCATTTGAGCTCAAAAAAGAAGCGGTCAAATTATGTGTGAAGCTATTCCTAGACCATGACAAAGAAACTCGAAAGAAATGGACGAATAAAATGATTGAATTACTCAAAAAGCAGACACTGCAATCATCCCTAATCAGTGAAGAACTCATCAGAGATACATTCCGTCAATGTAAATCAAAAGGAACCCAAGATGCGGGAAAACTGCTCAATGTATTCGACGCATTCTCCCTCCAGCCGTATGATTTTGATGCGGATCTTCGGAAAATGGTTCTAAGAAAAGAAAAAACTAGTCTGGCTGCTGATTCGTCGTCATTCTCACATGCTGCACGACAACGATTTTTTATTGTTAAGCAGCGTGCAATGCGATGTGCTTCACTGAAAAACTTCAAATTCACCACATGCGAAATTTTGGGAAGTTCCACAAAACATCTTCAATCAGTTGTAGTTCTCGGTATGCTTACTCAGCAAAAGGCCGACTGCTATCATATCGAAGATCTAAGCGGTTCAGTTGAAGTAGAATTTAAAGAAGACACGAAATTTCATCATGCGTTATTCCATGAGCATTCAATTGCAATTTTTGAAGGAACTTTTGAGAATAGTGTTTGAGCGGCCGC

Amino Acid Sequence

MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELVKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNNNNNNNNLGDDDDKVPEFLEVLFQGPLGSMDTTAEENALRREIVKTFQIHAFELKKEAVKLCVKLFLDHDKETRKKWTNKMIELLKKQTLQSSLISEELIRDTFRQCKSKGTQDAGKLLNVFDAFSLQPYDFDADLRKMVLRKEKTSLAADSSSFSHAARQRFFIVKQRAMRCASLKNFKFTTCEILGSSTKHLQSVVVLGMLTQQKADCYHIEDLSGSVEVEFKEDTKFHHALFHEHSIAIFEGTFENSV*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU62148
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: silent mutations A86
• Low Temp: No