cDNA Clone CDKL5

• Synonyms: EIEE2; ISSX; STK9
• Plasmid: pBabeD puro GFP NLS CDKL5
• Parent Plasmid: pBabeD puro GFP
• Expressed: GFP NLS CDKL5
• Genbank: NM_003159.2 GI:83367066*
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCccaccgaagaagaagcgcaaggtcATGAAGATTCCTAACATTGGTAATGTGATGAATAAATTTGAGAT
CCTTGGGGTTGTAGGTGAAGGAGCCTATGGAGTTGTACTTAAATGCAGAC
ACAAGGAAACACATGAAATTGTGGCGATCAAGAAATTCAAGGACAGTGAA
GAAAATGAAGAAGTCAAAGAAACGACTTTACGAGAGCTTAAAATGCTTCG
GACTCTCAAGCAGGAAAACATTGTGGAGTTGAAGGAAGCATTTCGTCGGA
GGGGAAAGTTGTACTTGGTGTTTGAGTATGTTGAAAAAAATATGCTCGAA
TTGCTGGAAGAAATGCCAAATGGAGTTCCACCTGAGAAAGTAAAAAGCTA
CATCTATCAGCTAATCAAGGCTATTCACTGGTGCCATAAGAATGATATTG
TCCATCGAGATATAAAACCAGAAAATCTCTTAATCAGCCACAATGATGTC
CTAAAACTGTGTGACTTTGGTTTTGCTCGTAATCTGTCAGAAGGCAATAA
TGCTAATTACACAGAGTACGTTGCCACCAGATGGTATCGGTCCCCAGAAC
TCTTACTTGGCGCTCCCTATGGAAAGTCCGTGGACATGTGGTCGGTGGGC
TGTATTCTTGGGGAGCTTAGCGATGGACAGCCTTTATTTCCTGGAGAAAG
TGAAATTGACCAACTTTTTACTATTCAGAAGGTGCTAGGACCACTTCCAT
CTGAGCAGATGAAGCTTTTCTACAGTAATCCTCGCTTCCATGGGCTCCGG
TTTCCAGCTGTTAACCATCCTCAGTCCTTGGAAAGAAGATACCTTGGAAT
TTTGAATAGTGTTCTACTTGACCTAATGAAGAATTTACTGAAGTTGGACC
CAGCTGACAGATACTTGACAGAACAGTGTTTGAATCACCCTACATTTCAA
ACCCAGAGACTTCTGGATCGTTCTCCTTCAAGGTCAGCAAAAAGAAAACC
TTACCATGTGGAAAGCAGCACATTGTCTAATAGAAACCAAGCCGGCAAAA
GCACTGCTTTGCAGTCTCACCACAGATCTAACAGCAAGGACATCCAGAAC
CTGAGTGTAGGCCTGCCCCGGGCTGACGAAGGTCTCCCTGCCAATGAAAG
CTTCCTAAATGGAAACCTTGCTGGAGCTAGTCTTAGTCCACTGCACACCA
AAACCTACCAAGCAAGCAGCCAGCCTGGGTCTACCAGCAAAGATCTCACC
AACAACAACATACCACACCTTCTTAGCCCAAAAGAAGCCAAGTCAAAAAC
AGAGTTTGATTTTAATATTGACCCAAAGCCTTCAGAAGGCCCAGGGACAA
AGTACCTCAAGTCAAACAGCAGATCTCAGCAGAACCGCCACTCATTCATG
GAAAGCTCTCAAAGCAAAGCTGGGACACTGCAGCCCAATGAAAAGCAGAG
TCGGCATAGCTATATTGACACAATTCCCCAGTCCTCTAGGAGTCCCTCCT
ACAGGACCAAGGCCAAAAGCCATGGGGCACTGAGTGACTCCAAGTCTGTG
AGCAACCTTTCTGAAGCCAGGGCCCAAATTGCGGAGCCCAGTACCAGTAG
GTACTTCCCATCTAGCTGCTTAGACTTGAATTCTCCCACCAGCCCAACCC
CCACCAGACACAGTGACACGAGAACTTTGCTCAGCCCTTCTGGAAGAAAT
AACCGAAATGAGGGAACGCTGGACTCACGTCGAACCACAACCAGACATTC
TAAGACGATGGAGGAATTGAAGCTGCCGGAGCACATGGACAGTAGCCATT
CCCATTCACTGTCTGCACCTCACGAATCTTTTTCTTATGGACTGGGCTAC
ACCAGCCCCTTTTCTTCCCAGCAACGTCCTCATAGGCATTCTATGTATGT
GACCCGTGACAAAGTGAGAGCCAAGGGCTTGGATGGAAGCTTGAGCATAG
GGCAAGGGATGGCAGCTAGAGCCAACAGCCTGCAACTCTTGTCACCCCAG
CCTGGAGAACAGCTCCCTCCAGAGATGACTGTGGCAAGATCTTCGGTCAA
AGAGACCTCCAGAGAAGGCACCTCTTCCTTCCATACACGCCAGAAGTCTG
AGGGTGGAGTGTATCATGACCCACACTCTGATGATGGCACAGCCCCCAAA
GAAAATAGACACCTATACAATGACCCTGTGCCAAGGAGAGTTGGTAGCTT
TTACAGAGTGCCATCTCCACGTCCAGACAATTCTTTCCATGAAAATAATG
TGTCAACTAGAGTTTCTTCTCTACCATCAGAGAGCAGTTCTGGAACCAAC
CACTCAAAAAGACAACCAGCATTCGATCCATGGAAAAGTCCTGAAAATAT
TAGTCATTCAGAGCAACTCAAGGAAAAAGAGAAGCAAGGATTTTTCAGGT
CAATGAAAAAGAAAAAGAAGAAATCTCAAACAGTACCCAATTCCGACAGC
CCTGATCTTCTGACGTTGCAGAAATCCATTCATTCTGCTAGCACTCCAAG
CAGCAGACCAAAGGAGTGGCGCCCCGAGAAGATCTCAGATCTGCAGACCC
AAAGCCAGCCATTAAAATCACTGCGCAAGTTGTTACATCTCTCTTCGGCC
TCAAATCACCCGGCTTCCTCAGATCCCCGCTTCCAGCCCTTAACAGCTCA
ACAAACCAAAAATTCCTTCTCAGAAATTCGGATTCACCCCCTGAGCCAGG
CCTCTGGCGGGAGCAGCAACATCCGGCAGGAACCCGCACCGAAGGGCAGG
CCAGCCCTCCAGCTGCCAGACGGTGGATGTGATGGCAGAAGACAGAGACA
CCATTCTGGACCCCAAGATAGACGCTTCATGTTAAGGACGACAGAACAAC
AAGGAGAATACTTCTGCTGTGGTGACCCAAAGAAGCCTCACACTCCGTGC
GTCCCAAACCGAGCCCTTCATCGTCCAATCTCCAGTCCTGCTCCCTATCC
AGTACTCCAGGTCCGAGGCACTTCCATGTGCCCGACACTCCAGGTCCGAG
GCACTGATGCTTTCAGCTGCCCAACCCAGCAATCCGGGTTCTCTTTCTTC
GTGAGACACGTTATGAGGGAAGCCCTGATTCACAGGGCCCAGGTAAACCA
AGCTGCGCTCCTGACATACCATGAGAATGCGGCACTGACGGGCAAGTGAG
CGGCCGC

Amino Acid Sequence

MHVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTI
FFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDN
HYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSPPKKKRKVMKIPNIGNVMNKFEILGVVGEGAYGVVLKCRHKETHEIVAIKKFKDS
EENEEVKETTLRELKMLRTLKQENIVELKEAFRRRGKLYLVFEYVEKNMLELLEEMPNGVPPEKVKSYIYQLIKAIHWCHKNDIVHRDIKPENLLISHND
VLKLCDFGFARNLSEGNNANYTEYVATRWYRSPELLLGAPYGKSVDMWSVGCILGELSDGQPLFPGESEIDQLFTIQKVLGPLPSEQMKLFYSNPRFHGL
RFPAVNHPQSLERRYLGILNSVLLDLMKNLLKLDPADRYLTEQCLNHPTFQTQRLLDRSPSRSAKRKPYHVESSTLSNRNQAGKSTALQSHHRSNSKDIQ
NLSVGLPRADEGLPANESFLNGNLAGASLSPLHTKTYQASSQPGSTSKDLTNNNIPHLLSPKEAKSKTEFDFNIDPKPSEGPGTKYLKSNSRSQQNRHSF
MESSQSKAGTLQPNEKQSRHSYIDTIPQSSRSPSYRTKAKSHGALSDSKSVSNLSEARAQIAEPSTSRYFPSSCLDLNSPTSPTPTRHSDTRTLLSPSGR
NNRNEGTLDSRRTTTRHSKTMEELKLPEHMDSSHSHSLSAPHESFSYGLGYTSPFSSQQRPHRHSMYVTRDKVRAKGLDGSLSIGQGMAARANSLQLLSP
QPGEQLPPEMTVARSSVKETSREGTSSFHTRQKSEGGVYHDPHSDDGTAPKENRHLYNDPVPRRVGSFYRVPSPRPDNSFHENNVSTRVSSLPSESSSGT
NHSKRQPAFDPWKSPENISHSEQLKEKEKQGFFRSMKKKKKKSQTVPNSDSPDLLTLQKSIHSASTPSSRPKEWRPEKISDLQTQSQPLKSLRKLLHLSS
ASNHPASSDPRFQPLTAQQTKNSFSEIRIHPLSQASGGSSNIRQEPAPKGRPALQLPDGGCDGRRQRHHSGPQDRRFMLRTTEQQGEYFCCGDPKKPHTP
CVPNRALHRPISSPAPYPVLQVRGTSMCPTLQVRGTDAFSCPTQQSGFSFFVRHVMREALIHRAQVNQAALLTYHENAALTGK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU58882
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: *Sequence difference compared with NM_003159.2 CDS:; t996c, t2118c (both silent)
• Low Temp: No