cDNA Clone FAM83D

• Plasmid: pBabeD puro GFP-FAM83D D249A
• Parent Plasmid: pBabeD puro GFP
• Expressed: GFP-FAM83D D249A
• Tag: GFP
• Species: Human

Sequence of Insert

ggatccATGGCTCTGCTGTCCGAGGGCCTGGACGAGGTGCCCGCCGCCTG
CCTGTCGCCGTGCGGGCCGCCCAACCCGACCGAGCTGTTCAGCGAGTCAC
GGCGCCTGGCTCTGGAGGAGCTGGTGGCGGGCGGCCCCGAAGCCTTCGCG
GCCTTCCTGCGACGCGAGCGCCTGGCTCGTTTCCTGAACCCCGATGAGGT
GCACGCCATTCTGCGCGCGGCGGAGAGGCCGGGAGAGGAGGGCGCGGCGG
CGGCGGCGGCGGCCGAGGACTCGTTCGGCTCCTCGCACGACTGCTCTTCG
GGCACCTACTTCCCCGAGCAGTCGGACCTGGAGCCACCGCTGTTGGAGCT
TGGCTGGCCCGCCTTCTACCAGGGCGCCTACCGCGGCGCCACGCGTGTCG
AGACGCACTTCCAGCCCCGCGGCGCTGGCGAAGGTGGCCCCTACGGCTGC
AAGGACGCTCTGCGCCAGCAGCTCCGCTCGGCGCGAGAGGTGATTGCAGT
GGTCATGGACGTGTTCACAGACATCGACATCTTCAGAGACCTGCAAGAAA
TATGCAGGAAACAGGGAGTTGCTGTGTATATCCTTCTGGACCAGGCTCTC
CTCTCTCAATTTCTGGATATGTGCATGGATCTGAAAGTTCATCCTGAACA
GGAAAAGTTAATGACAGTTCGGACTATCACAGGAAATATCTACTATGCAA
GGTCAGGAACTAAGATTATTGGGAAGGTTCACGAAAAGTTCACGTTGATT
GcTGGCATCCGCGTGGCAACAGGCTCCTACAGTTTTACATGGACGGATGG
CAAATTAAACAGCAGTAACTTGGTAATTCTGTCTGGCCAAGTGGTTGAAC
ACTTTGATCTGGAATTCCGAATCCTGTATGCCCAGTCCAAGCCCATCAGC
CCCAAACTCCTGTCTCACTTCCAGAGCAGCAACAAGTTTGATCACCTCAC
CAACCGAAAACCACAGTCCAAGGAGCTCACCCTGGGCAACCTGCTGCGGA
TGCGGCTGGCTAGGCTGTCAAGTACTCCCAGGAAGGCGGACCTGGACCCA
GAGATGCCCGCAGAGGGCAAGGCAGAGCGCAAGCCCCATGACTGTGAGTC
CTCTACTGTTAGTGAGGAAGACTACTTCAGCAGCCACAGGGACGAGCTCC
AGAGCAGAAAGGCCATTGACGCTGCCACTCAAACAGAGCCAGGAGAGGAG
ATGCCAGGGCTGAGTGTGAGTGAGGTGGGAACACAAACCAGCATCACCAC
AGCATGTGCTGGTACCCAGACTGCAGTCATCACCAGGATAGCAAGCTCTC
AAACCACGATTTGGTCCAGATCGACCACTACTCAGACTGACATGGATGAG
AACATTCTCTTTCCTCGAGGAACTCAATCTACAGAAGGGTCACCAGTCTC
AAAAATGTCTGTATCGAGATCTTCCAGTTTGAAGTCTTCCTCCTCTGTGT
CTTCCCAAGGCTCTGTGGCAAGCTCCACTGGTTCTCCCGCTTCCATCAGA
ACCACTGACTTCCACAATCCTGGCTATCCCAAGTACCTGGGCACCCCCCA
CCTGGAACTGTACTTGAGTGACTCACTTAGAAACTTGAACAAAGAGCGGC
AATTCCACTTCGCTGGTATCAGGTCCCGGCTCAACCACATGCTGGCTATG
CTGTCAAGGAGAACACTCTTTACTGAAAACCACCTTGGCCTTCATTCTGG
CAATTTCAGCAGAGTTAATTTGCTTGCTGTTAGAGATGTAGCACTTTATC
CTTCCTATCAGTAAgtcgacTCGCTCGAGAGTGCGGCCGC

Amino Acid Sequence

MHVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFIC
TTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTI
FFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSH
NVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDN
HYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMALLS
EGLDEVPAACLSPCGPPNPTELFSESRRLALEELVAGGPEAFAAFLRRER
LARFLNPDEVHAILRAAERPGEEGAAAAAAAEDSFGSSHDCSSGTYFPEQ
SDLEPPLLELGWPAFYQGAYRGATRVETHFQPRGAGEGGPYGCKDALRQQ
LRSAREVIAVVMDVFTDIDIFRDLQEICRKQGVAVYILLDQALLSQFLDM
CMDLKVHPEQEKLMTVRTITGNIYYARSGTKIIGKVHEKFTLIAGIRVAT
GSYSFTWTDGKLNSSNLVILSGQVVEHFDLEFRILYAQSKPISPKLLSHF
QSSNKFDHLTNRKPQSKELTLGNLLRMRLARLSSTPRKADLDPEMPAEGK
AERKPHDCESSTVSEEDYFSSHRDELQSRKAIDAATQTEPGEEMPGLSVS
EVGTQTSITTACAGTQTAVITRIASSQTTIWSRSTTTQTDMDENILFPRG
TQSTEGSPVSKMSVSRSSSLKSSSSVSSQGSVASSTGSPASIRTTDFHNP
GYPKYLGTPHLELYLSDSLRNLNKERQFHFAGIRSRLNHMLAMLSRRTLF
TENHLGLHSGNFSRVNLLAVRDVALYPSYQ*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU29393
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: delta aaM1-Ala30 with respect to full-length NCBI ref. ; N-terminally truncated predicted variant. Silent change at Glu316 (GAG > GAA); Parental 33932
• Low Temp: No