cDNA Clone FAM83D

• Plasmid: pBabeD puro-GFP-FAM83D
• Parent Plasmid: pBabeD puro-GFP
• Expressed: GFP-FAM83D
• Tag: GFP
• Species: Human

Sequence of Insert

ggatccATGGCTCTGCTGTCCGAGGGCCTGGACGAGGTGCCCGCCGCCTG CCTGTCGCCGTGCGGGCCGCCCAACCCGACCGAGCTGTTCAGCGAGTCAC GGCGCCTGGCTCTGGAGGAGCTGGTGGCGGGCGGCCCCGAAGCCTTCGCG GCCTTCCTGCGACGCGAGCGCCTGGCTCGTTTCCTGAACCCCGATGAGGT GCACGCCATTCTGCGCGCGGCGGAGAGGCCGGGAGAGGAGGGCGCGGCGG CGGCGGCGGCGGCCGAGGACTCGTTCGGCTCCTCGCACGACTGCTCTTCG GGCACCTACTTCCCCGAGCAGTCGGACCTGGAGCCACCGCTGTTGGAGCT TGGCTGGCCCGCCTTCTACCAGGGCGCCTACCGCGGCGCCACGCGTGTCG AGACGCACTTCCAGCCCCGCGGCGCTGGCGAAGGTGGCCCCTACGGCTGC AAGGACGCTCTGCGCCAGCAGCTCCGCTCGGCGCGAGAGGTGATTGCAGT GGTCATGGACGTGTTCACAGACATCGACATCTTCAGAGACCTGCAAGAAA TATGCAGGAAACAGGGAGTTGCTGTGTATATCCTTCTGGACCAGGCTCTC CTCTCTCAATTTCTGGATATGTGCATGGATCTGAAAGTTCATCCTGAACA GGAAAAGTTAATGACAGTTCGGACTATCACAGGAAATATCTACTATGCAA GGTCAGGAACTAAGATTATTGGGAAGGTTCACGAAAAGTTCACGTTGATT GATGGCATCCGCGTGGCAACAGGCTCCTACAGTTTTACATGGACGGATGG CAAATTAAACAGCAGTAACTTGGTAATTCTGTCTGGCCAAGTGGTTGAAC ACTTTGATCTGGAATTCCGAATCCTGTATGCCCAGTCCAAGCCCATCAGC CCCAAACTCCTGTCTCACTTCCAGAGCAGCAACAAGTTTGATCACCTCAC CAACCGAAAACCACAGTCCAAGGAGCTCACCCTGGGCAACCTGCTGCGGA TGCGGCTGGCTAGGCTGTCAAGTACTCCCAGGAAGGCGGACCTGGACCCA GAGATGCCCGCAGAGGGCAAGGCAGAGCGCAAGCCCCATGACTGTGAGTC CTCTACTGTTAGTGAGGAAGACTACTTCAGCAGCCACAGGGACGAGCTCC AGAGCAGAAAGGCCATTGACGCTGCCACTCAAACAGAGCCAGGAGAGGAG ATGCCAGGGCTGAGTGTGAGTGAGGTGGGAACACAAACCAGCATCACCAC AGCATGTGCTGGTACCCAGACTGCAGTCATCACCAGGATAGCAAGCTCTC AAACCACGATTTGGTCCAGATCGACCACTACTCAGACTGACATGGATGAG AACATTCTCTTTCCTCGAGGAACTCAATCTACAGAAGGGTCACCAGTCTC AAAAATGTCTGTATCGAGATCTTCCAGTTTGAAGTCTTCCTCCTCTGTGT CTTCCCAAGGCTCTGTGGCAAGCTCCACTGGTTCTCCCGCTTCCATCAGA ACCACTGACTTCCACAATCCTGGCTATCCCAAGTACCTGGGCACCCCCCA CCTGGAACTGTACTTGAGTGACTCACTTAGAAACTTGAACAAAGAGCGGC AATTCCACTTCGCTGGTATCAGGTCCCGGCTCAACCACATGCTGGCTATG CTGTCAAGGAGAACACTCTTTACTGAAAACCACCTTGGCCTTCATTCTGG CAATTTCAGCAGAGTTAATTTGCTTGCTGTTAGAGATGTAGCACTTTATC CTTCCTATCAGTAAgtcgac

Amino Acid Sequence

MHVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFIC
TTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTI
FFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSH
NVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDN
HYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMALLS
EGLDEVPAACLSPCGPPNPTELFSESRRLALEELVAGGPEAFAAFLRRER
LARFLNPDEVHAILRAAERPGEEGAAAAAAAEDSFGSSHDCSSGTYFPEQ
SDLEPPLLELGWPAFYQGAYRGATRVETHFQPRGAGEGGPYGCKDALRQQ
LRSAREVIAVVMDVFTDIDIFRDLQEICRKQGVAVYILLDQALLSQFLDM
CMDLKVHPEQEKLMTVRTITGNIYYARSGTKIIGKVHEKFTLIDGIRVAT
GSYSFTWTDGKLNSSNLVILSGQVVEHFDLEFRILYAQSKPISPKLLSHF
QSSNKFDHLTNRKPQSKELTLGNLLRMRLARLSSTPRKADLDPEMPAEGK
AERKPHDCESSTVSEEDYFSSHRDELQSRKAIDAATQTEPGEEMPGLSVS
EVGTQTSITTACAGTQTAVITRIASSQTTIWSRSTTTQTDMDENILFPRG
TQSTEGSPVSKMSVSRSSSLKSSSSVSSQGSVASSTGSPASIRTTDFHNP
GYPKYLGTPHLELYLSDSLRNLNKERQFHFAGIRSRLNHMLAMLSRRTLF
TENHLGLHSGNFSRVNLLAVRDVALYPSYQ*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU29395
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: delta aaM1-Ala30 with respect to full-length NCBI ref. ; N-terminally truncated predicted variant. Silent change at Glu316 (GAG > GAA); Parental 33932
• Low Temp: No