cDNA Clone ALC1

• Plasmid: pcDNA5D FRT/TO puro GFP ALC1
• Parent Plasmid: pcDNA5D FRT/TO puro GFP
• Expressed: GFP ALC1
• Genbank: AAO49505.1 with two silent changes
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCATGGAGCGCGCGGGCGCTACTAGCCGCGGGGGCCAAGCGCCTGGCTTCTTACTGCGGCTTCATACTGAGGGCCGAGCCGAGGCGGCGCGGGTGCAGGAGCAGGACTTACGGCAGTGGGGGCTGACAGGGATTCACCTACGCTCTTACCAGCTGGAGGGAGTAAACTGGCTCGCCCAGCGCTTCCATTGTCAGAATGGCTGTATCCTGGGAGATGAGATGGGCCTGGGGAAGACCTGCCAGACTATTGCTCTCTTCATTTATTTGGCAGGAAGATTAAATGATGAAGGGCCATTTCTGATTCTTTGTCCCTTGTCTGTTTTGAGCAACTGGAAAGAAGAAATGCAGAGATTTGCTCCAGGTCTTTCCTGTGTAACATATGCAGGCGACAAGGAGGAAAGAGCCTGCCTTCAGCAAGACCTGAAACAGGAGTCACGTTTTCATGTGCTACTGACTACCTATGAGATTTGCTTGAAAGATGCATCATTTCTAAAATCATTCCCTTGGAGTGTTCTTGTTGTGGATGAAGCTCACAGGTTGAAAAACCAAAGCTCCCTGCTGCATAAGACCTTGTCAGAGTTCTCAGTAGTCTTCAGTCTCCTGTTGACCGGAACTCCCATCCAGAACAGCCTCCAAGAGCTCTACTCCCTCCTCAGTTTTGTGGAGCCTGATCTCTTTTCCAAGGAAGAGGTGGGAGATTTTATTCAACGCTACCAGGATATTGAGAAAGAATCTGAGTCAGCAAGTGAACTGCACAAACTCTTGCAGCCATTTCTGCTGAGGCGAGTGAAAGCTGAGGTAGCTACAGAGCTTCCCAAGAAGACAGAAGTAGTGATATACCATGGCATGTCAGCATTGCAGAAGAAATACTACAAGGCCATTTTGATGAAAGACCTAGATGCATTTGAAAATGAGACGGCAAAGAAAGTTAAACTACAGAACATTTTGTCCCAGCTTCGAAAGTGTGTGGATCACCCATATTTGTTTGATGGTGTGGAGCCGGAGCCTTTTGAAGTTGGAGACCACCTGACTGAGGCTAGTGGGAAGCTTCACCTGCTGGATAAGCTACTAGCATTCCTGTATTCTGGGGGCCATCGGGTTTTACTTTTCTCCCAAATGACCCAGATGTTGGATATTCTCCAAGACTATATGGATTACAGAGGCTACAGCTATGAGCGTGTGGATGGTTCTGTGAGAGGAGAAGAGAGACACTTGGCCATTAAGAACTTTGGACAGCAGCCCATTTTCGTTTTTCTCCTGAGTACTAGGGCAGGTGGAGTTGGCATGAACTTAACAGCAGCAGATACTGTGATTTTTGTTGACAGTGACTTTAATCCTCAGAATGACTTGCAAGCAGCTGCCAGGGCTCATCGCATTGGCCAAAACAAGTCTGTTAAAGTTATTCGGCTGATTGGTCGAGACACTGTGGAAGAAATAGTCTATAGGAAAGCAGCCTCCAAACTGCAGCTCACCAACATGATCATAGAAGGAGGCCATTTTACTCTGGGAGCCCAGAAACCCGCTGCCGATGCTGACCTCCAGTTGAGTGAGATACTCAAATTTGGTTTGGATAAACTGCTGGCCTCTGAGGGGAGCACCATGGATGAAATAGACCTGGAGTCCATCCTGGGAGAAACAAAAGATGGCCAGTGGGTCTCTGATGCCTTGCCTGCAGCAGAAGGAGGGAGCAGAGATCAAGAGGAAGGAAAAAATCATATGTACTTATTTGAAGGTAAAGATTATTCTAAAGAGCCCAGTAAGGAAGACAGAAAATCATTTGAACAACTGGTAAACCTTCAGAAAACCCTTTTGGAGAAAGCTAGTCAAGAGGGCCGATCACTCCGAAATAAAGGCAGTGTTCTCATCCCAGGCCTTGTGGAGGGATCTACCAAAAGGAAGCGGGTTCTGAGTCCAGAAGAGCTGGAGGACAGACAGAAGAAAAGACAAGAAGCAGCTGCCAAGAGAAGGAGACTCATAGAGGAGAAGAAGAGGCAAAAGGAAGAGGCTGAACATAAGAAAAAGATGGCCTGGTGGGAATCCAACAATTACCAGTCCTTCTGCCTGCCCTCTGAGGAGAGCGAGCCAGAGGACCTTGAGAATGGGGAAGAGAGCTCTGCTGAGCTGGATTACCAAGACCCAGATGCTACTTCCCTCAAGTACGTTAGTGGTGATGTCACCCACCCTCAGGCTGGGGCCGAGGATGCTCTCATTGTGCACTGCGTAGATGACTCTGGCCACTGGGGCAGAGGTGGTTTATTTACAGCTCTGGAAAAGCGATCCGCTGAGCCAAGAAAAATATATGAGCTGGCTGGGAAAATGAAAGACCTGAGTTTGGGAGGTGTCCTTTTATTTCCTGTTGATGATAAAGAATCAAGAAACAAAGGGCAAGATTTGTTGGCCTTGATTGTGGCTCAGCATCGTGATCGTTCCAATGTCCTGTCTGGCATTAAGATGGCAGCCCTAGAAGAGGGCCTGAAGAAGATATTTTTAGCAGCAAAAAAGAAGAAAGCAAGTGTTCATCTTCCACGTATTGGACATGCCACGAAAGGTTTTAACTGGTATGGTACTGAGCGACTTATTCGGAAACATCTGGCTGCAAGAGGCATCCCAACTTACATATATTATTTTCCTAGAAGCAAGTCTGCTGTCCTTCATTCACAGTCTTCATCTTCCTCCTCAAGACAGCTGGTGCCTTAAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMERAGATSRGGQAPGFLLRLHTEGRAEAARVQEQDLRQWGLTGIHLRSYQLEGVNW
LAQRFHCQNGCILGDEMGLGKTCQTIALFIYLAGRLNDEGPFLILCPLSVLSNWKEEMQRFAPGLSCVTYAGDKEERACLQQDLKQESRFHVLLTTYEIC
LKDASFLKSFPWSVLVVDEAHRLKNQSSLLHKTLSEFSVVFSLLLTGTPIQNSLQELYSLLSFVEPDLFSKEEVGDFIQRYQDIEKESESASELHKLLQP
FLLRRVKAEVATELPKKTEVVIYHGMSALQKKYYKAILMKDLDAFENETAKKVKLQNILSQLRKCVDHPYLFDGVEPEPFEVGDHLTEASGKLHLLDKLL
AFLYSGGHRVLLFSQMTQMLDILQDYMDYRGYSYERVDGSVRGEERHLAIKNFGQQPIFVFLLSTRAGGVGMNLTAADTVIFVDSDFNPQNDLQAAARAH
RIGQNKSVKVIRLIGRDTVEEIVYRKAASKLQLTNMIIEGGHFTLGAQKPAADADLQLSEILKFGLDKLLASEGSTMDEIDLESILGETKDGQWVSDALP
AAEGGSRDQEEGKNHMYLFEGKDYSKEPSKEDRKSFEQLVNLQKTLLEKASQEGRSLRNKGSVLIPGLVEGSTKRKRVLSPEELEDRQKKRQEAAAKRRR
LIEEKKRQKEEAEHKKKMAWWESNNYQSFCLPSEESEPEDLENGEESSAELDYQDPDATSLKYVSGDVTHPQAGAEDALIVHCVDDSGHWGRGGLFTALE
KRSAEPRKIYELAGKMKDLSLGGVLLFPVDDKESRNKGQDLLALIVAQHRDRSNVLSGIKMAALEEGLKKIFLAAKKKKASVHLPRIGHATKGFNWYGTE
RLIRKHLAARGIPTYIYYFPRSKSAVLHSQSSSSSSRQLVP*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU58959
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Low Temp: No