cDNA Clone SLFN5

• Plasmid: pcDNA5D FRT/TO SLFN5 GFP
• Parent Plasmid: pcDNA5D FRT/TO C-GFP
• Expressed: SLFN5 GFP
• Genbank: NM_144975.3 *
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCGCCACCATGAGTCTTAGGATTGATGTGGATACAAACTTTCCTGAGTGTGTTGTAGATGCAGGAAAAGTCACCCTTGGGACTCAGCAGAGGCAGGAGATGGACCCTCGCCTGCGGGAGAAACAGAATGAAATCATCCTGCGAGCAGTATGTGCTCTGCTGAATTCTGGTGGGGGCATAATCAAGGCTGAGATTGAGAACAAAGGCTACAATTATGAACGTCATGGAGTAGGATTGGATGTGCCTCCAATTTTCAGAAGCCATTTAGATAAGATGCAGAAGGAAAACCACTTTTTGATTTTTGTGAAATCATGGAACACAGAGGCTGGTGTGCCACTTGCTACCTTATGCTCCAATTTGTACCACAGAGAGAGAACATCCACCGATGTCATGGATTCTCAGGAAGCTCTGGCATTCCTCAAATGCAGGACTCAGACTCCAACGAATATTAATGTTTCCAATTCATTAGGTCCACAGGCAGCTCAGGGTAGTGTACAATATGAAGGTAACATAAATGTGTCAGCTGCTGCTTTATTTGATAGAAAGCGGCTTCAGTATCTGGAAAAACTCAACCTTCCTGAGTCCACACATGTTGAATTTGTAATGTTCTCGACAGACGTGTCACACTGTGTTAAAGACAGACTTCCGAAGTGTGTTTCTGCATTTGCAAATACTGAAGGAGGATATGTATTTTTTGGTGTGCATGATGAGACTTGTCAAGTGATTGGATGTGAAAAAGAGAAAATAGACCTTACGAGCTTGAGGGCTTCTATTGATGGCTGTATTAAGAAGCTACCTGTCCATCATTTCTGCACACAGAGGCCTGAGATAAAATATGTCCTTAACTTCCTTGAAGTGCATGATAAGGGGGCCCTCCGTGGATATGTCTGTGCAATCAAGGTGGAGAAATTCTGCTGTGCGGTGTTTGCCAAAGTGCCTAGTTCCTGGCAGGTGAAGGACAACCGTGTGAGACAATTGCCCACAAGAGAATGGACTGCTTGGATGATGGAAGCTGACCCAGACCTTTCCAGGTGTCCTGAGATGGTTCTCCAGTTGAGTTTGTCATCTGCCACGCCCCGCAGCAAGCCTGTGTGCATTCATAAGAATTCGGAATGTCTGAAAGAGCAGCAGAAACGCTACTTTCCAGTATTTTCAGACAGAGTGGTATATACTCCAGAAAGCCTCTACAAGGAACTCTTCTCACAACATAAAGGACTCAGAGACTTAATAAATACAGAAATGCGCCCTTTCTCTCAAGGAATATTGATTTTTTCTCAAAGCTGGGCTGTGGATTTAGGTCTGCAAGAGAAGCAGGGAGTCATCTGTGATGCTCTTCTAATTTCCCAGAACAACACCCCTATTCTCTACACCATCTTCAGCAAGTGGGATGCGGGGTGCAAGGGCTATTCTATGATAGTTGCCTATTCTTTGAAGCAGAAGCTGGTGAACAAAGGCGGCTACACTGGGAGGTTATGCATCACCCCCTTGGTCTGTGTGCTGAATTCTGATAGAAAAGCACAGAGCGTTTACAGTTCGTATTTACAAATTTACCCTGAATCCTATAACTTCATGACCCCCCAGCACATGGAAGCCCTGTTACAGTCCCTCGTGATAGTCTTGCTTGGGTTCAAATCCTTCTTAAGTGAAGAGCTGGGCTCTGAGGTTTTGAACCTACTGACAAATAAACAGTATGAGTTGCTTTCAAAGAACCTTCGCAAGACCAGAGAGTTGTTTGTTCATGGCTTACCTGGATCAGGGAAGACTATCTTGGCTCTTAGGATCATGGAGAAGATCAGGAATGTGTTTCACTGTGAACCGGCTAACATTCTCTACATCTGTGAAAACCAGCCCCTGAAGAAGTTGGTGAGTTTCAGCAAGAAAAACATCTGCCAGCCAGTGACCCGGAAAACCTTCATGAAAAACAACTTTGAACACATCCAGCACATTATCATTGATGACGCTCAGAATTTCCGTACTGAAGATGGGGACTGGTATGGGAAAGCAAAGTTCATCACTCAGACAGCAAGGGATGGCCCAGGAGTTCTCTGGATCTTTCTGGACTACTTTCAGACCTATCACTTGAGTTGCAGTGGCCTCCCCCCTCCCTCAGACCAGTATCCAAGAGAAGAGATCAACAGAGTGGTCCGCAATGCAGGTCCAATAGCTAATTACCTACAACAAGTAATGCAGGAAGCCCGACAAAATCCTCCACCTAACCTCCCCCCTGGGTCCCTGGTGATGCTCTATGAACCTAAATGGGCTCAAGGTGTCCCAGGCAACTTAGAGATTATTGAAGACTTGAACTTGGAGGAGATACTGATCTATGTAGCGAATAAATGCCGTTTTCTCTTGCGGAATGGTTATTCTCTGAAGGATATTGCTGTGCTTTTCACCAAAGCAAGTGAAGTGGAAAAATATAAAGACAGGCTTCTAACAGCAATGAGGAAGAGAAAACTGTCTCAGCTCCATGAGGAGTCTGATCTGTTACTACAGATCGGTGATGCGTCGGATGTTCTAACCGATCACATTGTGTTGGACAGTGTCTGTCGATTTTCAGGCCTGGAAAGAAATATCGTGTTTGGAATCAATCCAGGAGTAGCCCCACCGGCTGGGGCCTACAATCTTCTGCTCTGTTTGGCTTCTAGGGCAAAAAGACATCTGTATATTCTGAAGGCTTCTGTGGCGGCCGC

Amino Acid Sequence

MSLRIDVDTNFPECVVDAGKVTLGTQQRQEMDPRLREKQNEIILRAVCALLNSGGGIIKAEIENKGYNYERHGVGLDVPPIFRSHLDKMQKENHFLIFVK
SWNTEAGVPLATLCSNLYHRERTSTDVMDSQEALAFLKCRTQTPTNINVSNSLGPQAAQGSVQYEGNINVSAAALFDRKRLQYLEKLNLPESTHVEFVMF
STDVSHCVKDRLPKCVSAFANTEGGYVFFGVHDETCQVIGCEKEKIDLTSLRASIDGCIKKLPVHHFCTQRPEIKYVLNFLEVHDKGALRGYVCAIKVEK
FCCAVFAKVPSSWQVKDNRVRQLPTREWTAWMMEADPDLSRCPEMVLQLSLSSATPRSKPVCIHKNSECLKEQQKRYFPVFSDRVVYTPESLYKELFSQH
KGLRDLINTEMRPFSQGILIFSQSWAVDLGLQEKQGVICDALLISQNNTPILYTIFSKWDAGCKGYSMIVAYSLKQKLVNKGGYTGRLCITPLVCVLNSD
RKAQSVYSSYLQIYPESYNFMTPQHMEALLQSLVIVLLGFKSFLSEELGSEVLNLLTNKQYELLSKNLRKTRELFVHGLPGSGKTILALRIMEKIRNVFH
CEPANILYICENQPLKKLVSFSKKNICQPVTRKTFMKNNFEHIQHIIIDDAQNFRTEDGDWYGKAKFITQTARDGPGVLWIFLDYFQTYHLSCSGLPPPS
DQYPREEINRVVRNAGPIANYLQQVMQEARQNPPPNLPPGSLVMLYEPKWAQGVPGNLEIIEDLNLEEILIYVANKCRFLLRNGYSLKDIAVLFTKASEV
EKYKDRLLTAMRKRKLSQLHEESDLLLQIGDASDVLTDHIVLDSVCRFSGLERNIVFGINPGVAPPAGAYNLLLCLASRAKRHLYILKASVAAAMVSKGE
ELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQS
ALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU63022
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Residue change compared with reference: P787L. Derives from RNA template. Also seen in EST sequences e.g. BC125201.1
• Low Temp: No