cDNA Clone PARKIN

• Plasmid: pEt15b 6HIS SUMO PARKIN M1-K76 D60N
• Parent Plasmid: pET15b 6His SUMO
• Expressed: 6HIS SUMO Parkin M1-K76 D60N
• Tag: 6His
• Tag: SUMO
• Species: Human

Sequence of Insert

ATGATAGTGTTTGTCAGGTTCAACTCCAGCCATGGTTTCCCAGTGGAGGTCGATTCTGACACCAGCATCTTCCAGCTCAAGGAGGTGGTTGCTAAGCGACAGGGGGTTCCGGCTGACCAGTTGCGTGTGATTTTCGCAGGGAAGGAGCTGAGGAATGACTGGACTGTGCAGAATTGTAACCTGGATCAGCAGAGCATTGTTCACATTGTGCAGAGACCGTGGAGAAAATAA

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQEQTGGMIVFVRFNSSHGFPVEVDSDTSIFQLKEVVAKRQGVPADQLRVIFAGKELRNDWTVQNCNLDQQSIVHIVQRPWRK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU62243
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Linker after SENP cleavage deleted making clone flush. 1-76 truncation made by inserting stop at aa77 in full length
• Low Temp: No