cDNA Clone Rilp

• Plasmid: pET15b 6HIS SUMO RILP A226-end
• Parent Plasmid: pET15b 6His SUMO
• Expressed: 6HIS SUMO RILP 226-end
• Tag: 6His
• Tag: SUMO
• Species: Human

Sequence of Insert

GAATTCGCGGAGGCCGCGCAGCAGCTCGGGCGCCCCTCGGAGGCAGGGCAGTGCCGCTTCAGTCGGGAGGAGTTTGAGCAGATCCTTCAGGAGCGGAATGAACTCAAAGCCAAAGTGTTCCTGCTCAAGGAGGAACTGGCCTACTTCCAGCGGGAGCTGCTCACAGACCACCGGGTCCCCGGCCTTCTGCTCGAGGCCATGAAGGTGGCTGTCCGGAAGCAGCGGAAGAAGATCAAGGCCAAGATGTTAGGGACACCAGAGGAAGCAGAGAGCAGTGAGGATGAGGCTGGCCCATGGATCCTGCTCTCCGATGACAAGGGAGACCATCCCCCACCCCCGGAGTCCAAAATACAGAGTTTCTTTGGCCTATGGTATCGGGGTAAAGCTGAATCCTCTGAGGATGAGACCAGCAGCCCTGCACCCAGCAAGCTAGGGGGAGAAGAGGAGGCCCAACCACAGTCTCCAGCTCCTGATCCGCCCTGTTCTGCCCTCCACGAACACCTTTGTCTGGGGGCCTCAGCCGCCCCAGAGGCCTGAGCGGCCGCG

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQEQTGGSGEFAEAAQQLGRPSEAGQCRFSREEFEQILQERNELKAKVFLLKEELAYFQRELLTDHRVPGLLLEAMKVAVRKQRKKIKAKMLGTPEEAESSEDEAGPWILLSDDKGDHPPPPESKIQSFFGLWYRGKAESSEDETSSPAPSKLGGEEEAQPQSPAPDPPCSALHEHLCLGASAAPEA*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU62300
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Needs linker after SENP cleavage to be deleted to make clone flush
• Low Temp: No