cDNA Clone FAM83D

• Plasmid: pcDNA5frtTO GST DU-FAM83D (shorter variant)
• Parent Plasmid: pcDNA5frtTO GST DU
• Expressed: GST-FAM83D
• Tag: GST
• Species: Human

Sequence of Insert

ggatccATGGCTCTGCTGTCCGAGGGCCTGGACGAGGTGCCCGCCGCCTG
CCTGTCGCCGTGCGGGCCGCCCAACCCGACCGAGCTGTTCAGCGAGTCAC
GGCGCCTGGCTCTGGAGGAGCTGGTGGCGGGCGGCCCCGAAGCCTTCGCG
GCCTTCCTGCGACGCGAGCGCCTGGCTCGTTTCCTGAACCCCGATGAGGT
GCACGCCATTCTGCGCGCGGCGGAGAGGCCGGGAGAGGAGGGCGCGGCGG
CGGCGGCGGCGGCCGAGGACTCGTTCGGCTCCTCGCACGACTGCTCTTCG
GGCACCTACTTCCCCGAGCAGTCGGACCTGGAGCCACCGCTGTTGGAGCT
TGGCTGGCCCGCCTTCTACCAGGGCGCCTACCGCGGCGCCACGCGTGTCG
AGACGCACTTCCAGCCCCGCGGCGCTGGCGAAGGTGGCCCCTACGGCTGC
AAGGACGCTCTGCGCCAGCAGCTCCGCTCGGCGCGAGAGGTGATTGCAGT
GGTCATGGACGTGTTCACAGACATCGACATCTTCAGAGACCTGCAAGAAA
TATGCAGGAAACAGGGAGTTGCTGTGTATATCCTTCTGGACCAGGCTCTC
CTCTCTCAATTTCTGGATATGTGCATGGATCTGAAAGTTCATCCTGAACA
GGAAAAGTTAATGACAGTTCGGACTATCACAGGAAATATCTACTATGCAA
GGTCAGGAACTAAGATTATTGGGAAGGTTCACGAAAAGTTCACGTTGATT
GATGGCATCCGCGTGGCAACAGGCTCCTACAGTTTTACATGGACGGATGG
CAAATTAAACAGCAGTAACTTGGTAATTCTGTCTGGCCAAGTGGTTGAAC
ACTTTGATCTGGAATTCCGAATCCTGTATGCCCAGTCCAAGCCCATCAGC
CCCAAACTCCTGTCTCACTTCCAGAGCAGCAACAAGTTTGATCACCTCAC
CAACCGAAAACCACAGTCCAAGGAGCTCACCCTGGGCAACCTGCTGCGGA
TGCGGCTGGCTAGGCTGTCAAGTACTCCCAGGAAGGCGGACCTGGACCCA
GAGATGCCCGCAGAGGGCAAGGCAGAGCGCAAGCCCCATGACTGTGAGTC
CTCTACTGTTAGTGAGGAAGACTACTTCAGCAGCCACAGGGACGAGCTCC
AGAGCAGAAAGGCCATTGACGCTGCCACTCAAACAGAGCCAGGAGAGGAG
ATGCCAGGGCTGAGTGTGAGTGAGGTGGGAACACAAACCAGCATCACCAC
AGCATGTGCTGGTACCCAGACTGCAGTCATCACCAGGATAGCAAGCTCTC
AAACCACGATTTGGTCCAGATCGACCACTACTCAGACTGACATGGATGAG
AACATTCTCTTTCCTCGAGGAACTCAATCTACAGAAGGGTCACCAGTCTC
AAAAATGTCTGTATCGAGATCTTCCAGTTTGAAGTCTTCCTCCTCTGTGT
CTTCCCAAGGCTCTGTGGCAAGCTCCACTGGTTCTCCCGCTTCCATCAGA
ACCACTGACTTCCACAATCCTGGCTATCCCAAGTACCTGGGCACCCCCCA
CCTGGAACTGTACTTGAGTGACTCACTTAGAAACTTGAACAAAGAGCGGC
AATTCCACTTCGCTGGTATCAGGTCCCGGCTCAACCACATGCTGGCTATG
CTGTCAAGGAGAACACTCTTTACTGAAAACCACCTTGGCCTTCATTCTGG
CAATTTCAGCAGAGTTAATTTGCTTGCTGTTAGAGATGTAGCACTTTATC
CTTCCTATCAGTAAgtcgacTCGCTCGAGAGTGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMALLSEGLDEVPAACLSPC
GPPNPTELFSESRRLALEELVAGGPEAFAAFLRRERLARFLNPDEVHAIL
RAAERPGEEGAAAAAAAEDSFGSSHDCSSGTYFPEQSDLEPPLLELGWPA
FYQGAYRGATRVETHFQPRGAGEGGPYGCKDALRQQLRSAREVIAVVMDV
FTDIDIFRDLQEICRKQGVAVYILLDQALLSQFLDMCMDLKVHPEQEKLM
TVRTITGNIYYARSGTKIIGKVHEKFTLIDGIRVATGSYSFTWTDGKLNS
SNLVILSGQVVEHFDLEFRILYAQSKPISPKLLSHFQSSNKFDHLTNRKP
QSKELTLGNLLRMRLARLSSTPRKADLDPEMPAEGKAERKPHDCESSTVS
EEDYFSSHRDELQSRKAIDAATQTEPGEEMPGLSVSEVGTQTSITTACAG
TQTAVITRIASSQTTIWSRSTTTQTDMDENILFPRGTQSTEGSPVSKMSV
SRSSSLKSSSSVSSQGSVASSTGSPASIRTTDFHNPGYPKYLGTPHLELY
LSDSLRNLNKERQFHFAGIRSRLNHMLAMLSRRTLFTENHLGLHSGNFSR
VNLLAVRDVALYPSYQ*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU29540
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: delta aaM1-Ala30 with respect to full-length NCBI ref. N-terminally truncated predicted variant. Silent change at Glu316 (GAG > GAA); Parental 41851
• Low Temp: No