cDNA Clone Histone H4

• Synonyms: H4FA; HIST1H4A; H4
• Plasmid: pcDNA5D FRT/TO mCherry Histone H4 R18A*
• Parent Plasmid: pcDNA5D FRT/TO mCherry
• Expressed: mCherry-Histone H4 R18A*
• Genbank: NM_003538.3 (P62805)
• Tag: mCherry
• Species: Human

Sequence of Insert

GGATCCATGTCTGGACGTGGTAAGGGCGGGAAGGGTTTGGGTAAGGGGGGTGCCAAGGCCCACCGCAAGGTGTTGCGTGACAACATCCAGGGCATCACCAAGCCGGCCATCCGGCGTCTGGCCCGGCGTGGCGGTGTGAAGCGGATCTCTGGTCTGATCTACGAGGAGACTCGCGGGGTGCTCAAGGTGTTTTTGGAGAACGTGATCCGTGACGCTGTCACCTATACGGAGCACGCCAAGCGCAAGACAGTCACTGCCATGGACGTGGTCTACGCGCTTAAGCGCCAGGGACGCACCCTTTATGGCTTTGGCGGTTAAGCGGCCGC

Amino Acid Sequence

MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWER
VMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNV
NIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYKSGLGSMSGRGKGGKGLGKGGAKAHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVL
KVFLENVIRDAVTYTEHAKRKTVTAMDVVYALKRQGRTLYGFGG*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU63227
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: * The initiator methionine residues of all four core histones are co-translationally removed from the nascent polypeptides by methionine aminopeptidase.; The first actual amino acid of H4 is the first Serine, which means that the R18A mutation in this clone is actually R17A in the expressed polypeptide.
• Low Temp: No