cDNA Clone TIF4631 (YGR162W)

• Plasmid: pRS413 ADH Pr -600-TIF4631-(+)300
• Parent Plasmid: pRS413 ADH Pr
• Expressed: yeast TIF4631 (YGR162W) including 600 bp promoter and 300 bp 3'UTR

Sequence of Insert

GGATCCTGGATCAATCTTCCAATAAGAGTGGTTTCGTGGTCTAGTCGGTTATGGCATCTGCTTAACACGCAGAACGTCCCCAGTTCGATCCTGGGCGAAATCATTTTTTTGAAAATTACATTAATAAGGCTTTTTTCAATATCTCTGGAACAACAGTTTGTTTCTACTTACTAATAGCTTTAAGGACCCTCTTGGACATCATGATGGCAGACTTCCATCGTAGTGGGATGATCATATGATGGGCGCTATCCTCATCGCGACTCGATAACGACGTGAGAAACGATTTTTTTTTTTTTTCTTTTTCACCGTATTTTTGTGCGTCCTTTTTCAATTATAGCTTTTTTTTATTTTTTTTTTTTCTCGTACTGTTTCACTGACAAAAGTTTTTTTTCAAGAAAAATTTTCGATGCCGCGTTCTCTGTGTGCAACGGATGGATGGTAGATGGAATTTCAATATGTTGCTTGAAATTTTACCAATCTTGATATTGTGATAATTTACTTAATTATGATTCTTCCTCTTCCCTTCAATTTCTTAAAGCTTCTTACTTTACTCCTTCTTGCTCATAAATAAGCAAGGTAAGAGGACAACTGTAATTACCTATTACAATAATGACAGACGAAACTGCTCAACCGACACAATCTGCTTCAAAGCAGGAATCTGCTGCTCTGAAACAGACAGGCGATGACCAGCAGGAATCTCAGCAACAACGTGGCTACAACAACTACAACAATGGTAGCAACTATACCCAAAAGAAGCCTTACAACAGCAACAGACCTCATCAGCAAAGAGGTGGTAAATTTGGACCAAACAGATATAACAACCGTGGCAACTATAATGGTGGCGGTAGTTTCAGAGGTGGACACATGGGAGCCAACAGCTCAAACGTGCCATGGACTGGTTACTATAATAACTACCCCGTTTACTACCAGCCCCAGAAAATGGCGGCCGCTGGTAGTGCCCCTGCTAATCCAATTCCTGTCGAAGAGAAGTCACCTGTTCCAACTAAGATAGAAATCACTACCAAGTCTGGTGAACACTTAGATTTGAAAGAACAGCATAAAGCCAAGCTACAGTCTCAGGAAAGATCTACTGTGTCTCCGCAACCAGAGTCAAAGTTAAAAGAAACTTCTGATTCTACTTCTACTTCTACTCCAACTCCTACCCCTTCCACTAATGACTCTAAGGCCAGTTCTGAAGAAAATATATCTGAAGCTGAAAAGACAAGAAAAAATTTCATCGAGCAAGTTAAACTTCGTAAAGCTGCTTTAGAAAAGAAGAGAAAGGAGCAACTTGAAGGTTCTAGTGGCAACAATAATATTCCAATGAAGACTACCCCAGAAAATGTGGAAGAGAAGGGATCGGACAAACCTGAGGTAACCGAAAAAACCAAGCCTGCTGAAGAAAAATCTGCTGAGCCAGAAGTTAAGCAAGAAACTCCAGCTGAAGAAGGTGAACAAGGAGAAAAGGGCCAAATAAAGGAAGAATCCACTCCAAAGGTGTTAACCTTTGCTGAACGTTTGAAGTTGAAGAAACAACAAAAAGAACGAGAAGAAAAAACGGAGGGGAAGGAAAACAAGGAAGTGCCAGTACAGGAAGAGACTAAGAGCGCCATCGAGTCTGCTCCTGTGCCTCCCAGTGAACAGGTTAAAGAAGAAACTGAGGTCGCCGAAACCGAACAGTCAAACATTGAAGAATCTGCAACAACTCCGGCTATCCCTACTAAATCTGATGAAGCTGAAGCTGAAGTTGAGGCTGAAGCAGGTGATGCTGGAACAAAGATTGGACTTGAAGCTGAAATCGAAACTACAACTGATGAAACTGATGATGGTACAAACACCGTTTCTCACATATTGAATGTATTGAAAGATGCTACACCAATTGAAGATGTCTTTTCTTTTAACTATCCAGAAGGTATTGAAGGTCCTGATATCAAATACAAGAAAGAACACGTCAAATATACATATGGCCCAACTTTCTTGCTTCAATTTAAGGACAAACTAAACGTCAAAGCGGACGCCGAATGGGTCCAAAGCACTGCTTCTAAAATTGTCATTCCACCAGGAATGGGCAGAGGAAATAGATCTAGAGATTCTGGCAGATTCGGCAATAATTCTAGTCGTGGCCATGACTTTAGAAATACCTCAGTGAGAAATATGGATGACAGAGCTAATTCAAGAACTTCATCAAAGAGAAGATCAAAGAGAATGAATGACGACAGGAGATCTAATAGATCTTATACATCAAGAAGAGACCGCGAGAGAGGCTCCTACAGAAATGAAGAGAAAAGAGAGGACGATAAACCAAAGGAAGAAGTTGCTCCACTTGTTCCAAGTGCTAATAGGTGGGTGCCAAAATTCAAGTCTAAAAAGACTGAAAAGAAGCTAGCCCCTGACGGAAAGACCGAACTATTGGACAAGGATGAAGTTGAAAGAAAGATGAAGTCACTACTGAATAAACTTACTTTGGAAATGTTCGATGCTATTTCATCTGAAATTTTGGCTATTGCAAACATATCAGTATGGGAAACAAATGGTGAAACGTTAAAGGCTGTGATAGAACAGATTTTCTTAAAGGCCTGTGACGAACCCCATTGGTCTTCTATGTACGCGCAATTATGTGGTAAAGTAGTTAAAGAATTAAACCCAGATATAACAGATGANACTAATGAAGGTAAGACAGGTCCAAAATTGGTTTTGCATTACTTGGTTGCTAGATGTCATGCCGAATTTGACAAGGGTTGGACCGATAAATTACCCACAAACGAAGATGGTACTCCATTAGAACCTGAGATGATGTCAGAAGAGTACTATGCAGCTGCTTCTGCTAAGAGAAGAGGTTTAGGTTTGGTTCGTTTCATCGGTTTCCTATACCGTCTGAATCTGTTGACTGGAAAGATGATGTTCGAATGTTTCCGTAGACTAATGAAGGATTTGACGGATTCTCCATCTGAAGAAACTCTAGAGTCCGTTGTTGAATTGTTAAATACTGTTGGTGAACAATTTGAGACCGATAGTTTCAGAACGGGTCAAGCAACATTGGAGGGTTCGCAATTGCTCGATAGTTTGTTCGGTATTTTAGACAATATTATTCAAACTGCTAAGATTTCTAGTAGAATCAAGTTCAAGTTGATCGACATTAAAGAATTAAGGCACGACAAGAACTGGAACAGTGATAAGAAGGACAACGGTCCTAAGACCATTCAACAGATTCATGAGGAAGAGGAGAGACAACGTCAATTGAAGAATAATTCAAGATCTAATTCAAGACGTACGAACAACTCCAGTAATAGACACTCTTTCAGAAGGGACGCTCCTCCTGCATCAAAAGATAGCTTTATCACAACCAGAACATATTCTCAAAGAAATAGCCAAAGGGCTCCTCCTCCAAAGGAAGAACCAGCTGCACCAACTTCTACCGCAACAAATATGTTCAGTGCATTAATGGGAGAAAGTGATGACGAAGAGTAACATGAATAGATCATGTTAAGTATCGAAAATGTCACTTGGATACAAGGATGTTGGTAGAAAGTCTAATAAAATAATAAATTAATAGTACAACACAAATTTAATCTTACGTTTAATCCTGCGCTATTAATTGAAAATATATGCATGGAGAAGAAAAAAAAAGTGTCCCTAATTTACCATTGTTATATTTTCTCGATTAATGGGCTCATTTCTTTTCACTAAAAGCTATAAACAAGTTTGATGCATTTTAATGGGTAATCCTCTTACTGTGCTTTTTTTTCCTATCTTGTTCTATATGTCAATGAATTC

Amino Acid Sequence

MTDETAQPTQSASKQESAALKQTGDDQQESQQQRGYNNYNNGSNYTQKKPYNSNRPHQQRGGKFGPNRYNNRGNYNGGGSFRGGHMGANSSNVPWTGYYNNYPVYYQPQKMAAAGSAPANPIPVEEKSPVPTKIEITTKSGEHLDLKEQHKAKLQSQERSTVSPQPESKLKETSDSTSTSTPTPTPSTNDSKASSEENISEAEKTRKNFIEQVKLRKAALEKKRKEQLEGSSGNNNIPMKTTPENVEEKGSDKPEVTEKTKPAEEKSAEPEVKQETPAEEGEQGEKGQIKEESTPKVLTFAERLKLKKQQKEREEKTEGKENKEVPVQEETKSAIESAPVPPSEQVKEETEVAETEQSNIEESATTPAIPTKSDEAEAEVEAEAGDAGTKIGLEAEIETTTDETDDGTNTVSHILNVLKDATPIEDVFSFNYPEGIEGPDIKYKKEHVKYTYGPTFLLQFKDKLNVKADAEWVQSTASKIVIPPGMGRGNRSRDSGRFGNNSSRGHDFRNTSVRNMDDRANSRTSSKRRSKRMNDDRRSNRSYTSRRDRERGSYRNEEKREDDKPKEEVAPLVPSANRWVPKFKSKKTEKKLAPDGKTELLDKDEVERKMKSLLNKLTLEMFDAISSEILAIANISVWETNGETLKAVIEQIFLKACDEPHWSSMYAQLCGKVVKELNPDITDXTNEGKTGPKLVLHYLVARCHAEFDKGWTDKLPTNEDGTPLEPEMMSEEYYAAASAKRRGLGLVRFIGFLYRLNLLTGKMMFECFRRLMKDLTDSPSEETLESVVELLNTVGEQFETDSFRTGQATLEGSQLLDSLFGILDNIIQTAKISSRIKFKLIDIKELRHDKNWNSDKKDNGPKTIQQIHEEEERQRQLKNNSRSNSRRTNNSSNRHSFRRDAPPASKDSFITTRTYSQRNSQRAPPPKEEPAAPTSTATNMFSALMGESDDEE*

• Vector Type: Yeast
• Antibiotic: Amp
• DU Number: DU59581
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Cloning of the budding yeast TIF4631 (YGR162W) including 600 bp from its own promoter and 300 bp from its own 3'UTR in the pRS413 centromeric vector.
• Low Temp: No