cDNA Clone Pdpc1

• Synonyms: PDH; PDP; PDPC; PPM2A; PPM2C
• Plasmid: pMEX3Cb PDPC1
• Parent Plasmid: pMEX3Cb
• Expressed: MBP 3C PDPC1
• Genbank: NM_018444 (see notes)
• Tag: MBP
• Species: Human

Sequence of Insert

ggatccTTGTCGGCTCCGTGTTGTGATGACAGGAGAATGTGTGTGTGTCCCGGGCCCAGACGAATTGGAATCCCAGTCAGAAGTTCCAGCCTGCCACTGTTCTCTGATGCCATGCCAGCACCAACTCAACTGTTTTTTCCTCTCATCCGTAAC
TGTGAACTGAGCAGGATCTATGGCACTGCATGTTACTGCCACCACAAACA
TCTCTGTTGTTCCTCATCGTACATTCCTCAGAGTCGACTGAGATACACAC
CTCATCCAGCATATGCTACCTTTTGCAGGCCAAAGGAGAACTGGTGGCAG
TACACCCAAGGAAGGAGATATGCTTCCACACCACAGAAATTTTACCTCAC
ACCTCCACAAGTCAATAGCATCCTTAAAGCTAATGAATACAGTTTCAAAG
TGCCAGAATTTGACGGCAAAAATGTCAGTTCTATCCTTGGATTTGACAGC
AATCAGCTGCCTGCAAATGCACCCATTGAGGACCGGAGAAGTGCAGCAAC
CTGCTTGCAGACCAGAGGGATGCTTTTGGGGGTTTTTGATGGCCATGCAG
GTTGTGCTTGTTCCCAGGCAGTCAGTGAAAGACTCTTTTATTATATTGCT
GTCTCTTTGTTACCCCATGAGACTTTGCTAGAGATTGAAAATGCAGTGGA
GAGCGGCCGGGCACTGCTACCCATTCTCCAGTGGCACAAGCACCCCAATG
ATTACTTTAGTAAGGAGGCATCCAAATTGTACTTTAACAGCTTGAGGACT
TACTGGCAAGAGCTTATAGACCTCAACACTGGTGAGTCGACTGATATTGA
TGTTAAGGAGGCTCTAATTAATGCCTTCAAGAGGCTTGATAATGACATCT
CCTTGGAGGCGCAAGTTGGTGATCCTAATTCTTTTCTCAACTACCTGGTG
CTTCGAGTGGCATTTTCTGGAGCCACTGCTTGTGTGGCCCATGTGGATGG
TGTTGACCTTCATGTGGCCAATACTGGCGATAGCAGAGCCATGCTGGGTG
TGCAGGAAGAGGACGGCTCATGGTCAGCAGTCACGCTGTCTAATGACCAC
AATGCTCAAAATGAAAGAGAACTAGAACGGCTGAAATTGGAACATCCAAA
GAGTGAGGCCAAGAGTGTCGTGAAACAGGATCGGCTGCTTGGCTTGCTGA
TGCCATTTAGGGCATTTGGAGATGTAAAGTTCAAATGGAGCATTGACCTT
CAAAAGAGAGTGATAGAATCTGGCCCAGACCAGTTGAATGACAATGAATA
TACCAAGTTTATTCCTCCTAATTATCACACACCTCCTTATCTCACTGCTG
AGCCAGAGGTAACTTACCACCGATTAAGGCCACAGGATAAGTTTCTGGTG
TTGGCTACTGATGGGTTGTGGGAGACTATGCATAGGCAGGATGTGGTTAG
GATTGTGGGTGAGTACCTAACTGGCATGCATCACCAACAGCCAATAGCTG
TTGGTGGCTACAAGGTGACTCTGGGACAGATGCATGGCCTTTTAACAGAA
AGGAGAACCAAAATGTCCTCGGTATTTGAGGATCAGAACGCAGCAACCCA
TCTCATTCGCCACGCTGTGGGCAACAACGAGTTTGGGACTGTTGATCATG
AGCGCCTCTCTAAAATGCTTAGTCTTCCTGAAGAGCTTGCTCGAATGTAC
AGAGATGACATTACAATCATTGTAGTTCAGTTCAATTCTCATGTTGTAGG
GGCGTATCAAAACCAAGAAAAGTGAgaattc

Amino Acid Sequence

MMKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVR
YNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDL
IKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKP
LGAVALKSYEEELVKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNNNNNNNNLGDDDDKVPEFLEVLFQG
PLGSLSAPCCDDRRMCVCPGPRRIGIPVRSSSLPLFSDAMPAPTQLFFPLIRNCELSRIYGTACYCHHKHLCCSSSYIPQSRLRYTPHPAYATFCRPKEN
WWQYTQGRRYASTPQKFYLTPPQVNSILKANEYSFKVPEFDGKNVSSILGFDSNQLPANAPIEDRRSAATCLQTRGMLLGVFDGHAGCACSQAVSERLFY
YIAVSLLPHETLLEIENAVESGRALLPILQWHKHPNDYFSKEASKLYFNSLRTYWQELIDLNTGESTDIDVKEALINAFKRLDNDISLEAQVGDPNSFLN
YLVLRVAFSGATACVAHVDGVDLHVANTGDSRAMLGVQEEDGSWSAVTLSNDHNAQNERELERLKLEHPKSEAKSVVKQDRLLGLLMPFRAFGDVKFKWS
IDLQKRVIESGPDQLNDNEYTKFIPPNYHTPPYLTAEPEVTYHRLRPQDKFLVLATDGLWETMHRQDVVRIVGEYLTGMHHQQPIAVGGYKVTLGQMHGL
LTERRTKMSSVFEDQNAATHLIRHAVGNNEFGTVDHERLSKMLSLPEELARMYRDDITIIVVQFNSHVVGAYQNQEK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU63288
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N.B. sequence changes compared with NM_018444 CDS: ; Single nucleotide insertion after nt 421 (causing frame shift).; Single nucleotide deletion at a454 (frame reverts back to original).; Point mutations g561t (W187C), g602t (G201V), g610t (V204L). ; Same sequence as in pGEX6P1 PDPC1 clone DU1375.; Changes present in all available EST sequences, therefore assumed to be correct.
• Low Temp: No