cDNA Clone FAM83H

• Plasmid: pBabeD puro GFP-FAM83H
• Parent Plasmid: pBabeD puro GFP
• Expressed: GFP-FAM83H
• Genbank: NM_198488.3
• Tag: GFP
• Species: Human

Sequence of Insert

ggatccatggcccgtcgctctcagagctcctcgcagggggacaacccact
ggcacccgggtacctgccgcctcactacaaagagtactaccgcctggcgg
tggatgcactggccgagggtggctcggaggcctacagccgcttcctcgct
accgagggggcaccagacttcctgtgccctgaagagctggaacatgtgag
ccgacaccttcggcctccgcagtatgttacccgagagccacctgaaggca
gccttctcgacgtggacatggatggctcctcgggtacatactggccagtg
aactcagaccaggccgtgcctgagcttgatttgggctggcctctgacctt
cggcttccagggcaccgaggtgaccaccttggtgcagccaccgccccccg
acagccccagtatcaaggatgaggcccgcaggatgatccgttccgcccag
caggtggtggccgtggtgatggacatgttcactgatgtggacctgctcag
cgaagtgctggaggccgcggcccgtcgggtcccagtctacatcctgctgg
atgagatgaacgcgcagcacttcctggacatggccgacaagtgccgtgtc
aacctgcaccacgtggatttcctgcgcgtacggactgtggcgggccccac
ctactactgccgcactgggaagtccttcaagggccacgtcaaggagaagt
tcctgctggtggactgtgccgtggtgatgagtgggagctacagcttcatg
tggtcctttgagaagatccaccgcagcctggcgcacgtgttccaaggaga
gctggtctccagcttcgacgaggagttccgcatcctcttcgcgcagtccg
agccgcttgtgccctcggccgcggccctggcccgcatggacgcctatgcc
ctggctccgtatgccggggccgggcctctcgtgggcgtccctggggtcgg
ggcgccaacccccttctccttccctaaacgagcgcacctcctgttcccgc
caccccgggaagagggcctgggcttcccctccttcctcgacccggaccgc
cacttcctgtcggccttccgccgggaggagccgccgcggatgccgggggg
cgcgctggaaccgcacgcggggctgcggccgctctcgcggcgcctggagg
ccgaggccgggccggctggggagctcgcgggcgcgcggggcttcttccag
gcgcggcacctggagatggacgccttcaagcggcacagcttcgcgaccga
gggcgcgggcgccgtggagaacttcgcggccgcgcggcaggtgtcgcggc
agacgttcctcagccacggcgacgacttccgcttccagaccagccacttc
caccgtgaccagctctaccagcagcagtaccagtgggacccgcagctcac
gccggcgcgcccgcaaggcctgttcgagaagcttcgcgggggccgcgcgg
gtttcgcggacccggatgacttcaccctgggcgccgggccccgcttcccg
gagctcggacccgacgggcaccagcggctggactacgtgccgtccagcgc
gtcccgcgaggtgcgccacggctcggaccccgccttcgcgcccggacccc
gcggcctggagcccagcggagccccgcgccccaacctgacccagcgcttc
ccatgccaggccgcggcgaggccgggcccagaccccgctcccgaggcgga
gccggagcgcaggggcgggcccgaggggcgggcagggctgcggcgctggc
gtttggcctcctacttgagcggctgccacggcgaggatgggggcgacgac
ggcctaccggcgcccatggaagcggaggcttacgaagacgacgtgctggc
tcccgggggccgggcacctgccggcgacctgctcccctcggccttccgcg
tcccagcagccttccccaccaaggtcccggtgccaggcccgggcagcggc
ggcaacggcccagagcgcgagggcccggaggagcctggcctggccaagca
ggactcattccgctcgcgcctgaaccccctggtccagcgcagctccaggc
tgcgctcctcgctcatcttcagcacgtcacaggccgagggcgcggccggg
gctgcggcggccactgagaaggtgcagctgctgcacaaggagcagacggt
cagcgagacgctggggcccggcggagaggccgtgcgctccgcggcttcca
ccaaggtggcggagctgctggagaagtacaagggcccagcccgtgatccc
ggcggcggcgcgggcgccatcaccgttgccagccacagcaaggccgtcgt
gtcccaggcgtggcgggaagaggtggcggccccaggtgccgtggggggcg
agcgccgcagcctcgagagctgcctgctggacctgcgcgactcctttgca
cagcagctgcaccaggaggcggagcggcagccgggagccgcgtcgctcac
cgcggcgcagctgctcgacacactgggccggagcggctccgaccgcctgc
cttcccgcttcctctctgcccagagccactcaacgtccccgcaagggctg
gacagccctctgccgctggaagggtccggagcgcaccaggtgctccataa
tgagtcaaaagggagccccacctcggcttaccctgagcggaaggggagcc
ccacgcctgggttttccactcgaagaggaagtccaactacaggatttatc
gagcagaaggggagccccacctcagcctaccccgagcgcaggggtagtcc
ggtgccccccgtgccggagcgcaggagcagtccggtgccccccgtgccgg
agcgcaggggcagcctcacccttaccatctccggggagtccccgaaggcc
gggcccgcggaggaggggccgagcggccccatggaagtcctgcgcaaagg
ctccttgcgtcttaggcagctgctgagccccaagggcgagcggcgcatgg
aggatgagggtggcttcccagtgccgcaggagaacggccaacccgagagc
ccgcggcgtctgtcactgggccagggtgacagcacggaggctgccacaga
agagcggggtccgcgggcgcgcctgtcctcagccacggccaacgccttgt
acagcagcaaccttcgggatgacacgaaggccattctggagcagatcagt
gcccacggccagaagcaccgtgcggtccctgccccgagccccggcccgac
ccacaacagccccgagctaggccgtccaccggctgctggcgtcctggccc
cagatatgtccgacaaggacaagtgttcagccatcttccgctcggacagc
ttggggacccagggccggctgagccgcacgctgccagccagcgcggagga
gcgcgatcggctgctgcgccgcatggagagcatgcgcaaggagaagcgcg
tgtacagccgcttcgaggtcttctgcaagaaagaggaggccagcagccct
ggggcaggggaaggccccgcggaggagggcaccagggacagcaaggtggg
caagttcgtgcccaagatcctgggcacgttcaaaagcaagaagtgagcgg
ccgc

Amino Acid Sequence

MHVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFIC
TTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTI
FFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSH
NVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDN
HYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMARRS
QSSSQGDNPLAPGYLPPHYKEYYRLAVDALAEGGSEAYSRFLATEGAPDF
LCPEELEHVSRHLRPPQYVTREPPEGSLLDVDMDGSSGTYWPVNSDQAVP
ELDLGWPLTFGFQGTEVTTLVQPPPPDSPSIKDEARRMIRSAQQVVAVVM
DMFTDVDLLSEVLEAAARRVPVYILLDEMNAQHFLDMADKCRVNLHHVDF
LRVRTVAGPTYYCRTGKSFKGHVKEKFLLVDCAVVMSGSYSFMWSFEKIH
RSLAHVFQGELVSSFDEEFRILFAQSEPLVPSAAALARMDAYALAPYAGA
GPLVGVPGVGAPTPFSFPKRAHLLFPPPREEGLGFPSFLDPDRHFLSAFR
REEPPRMPGGALEPHAGLRPLSRRLEAEAGPAGELAGARGFFQARHLEMD
AFKRHSFATEGAGAVENFAAARQVSRQTFLSHGDDFRFQTSHFHRDQLYQ
QQYQWDPQLTPARPQGLFEKLRGGRAGFADPDDFTLGAGPRFPELGPDGH
QRLDYVPSSASREVRHGSDPAFAPGPRGLEPSGAPRPNLTQRFPCQAAAR
PGPDPAPEAEPERRGGPEGRAGLRRWRLASYLSGCHGEDGGDDGLPAPME
AEAYEDDVLAPGGRAPAGDLLPSAFRVPAAFPTKVPVPGPGSGGNGPERE
GPEEPGLAKQDSFRSRLNPLVQRSSRLRSSLIFSTSQAEGAAGAAAATEK
VQLLHKEQTVSETLGPGGEAVRSAASTKVAELLEKYKGPARDPGGGAGAI
TVASHSKAVVSQAWREEVAAPGAVGGERRSLESCLLDLRDSFAQQLHQEA
ERQPGAASLTAAQLLDTLGRSGSDRLPSRFLSAQSHSTSPQGLDSPLPLE
GSGAHQVLHNESKGSPTSAYPERKGSPTPGFSTRRGSPTTGFIEQKGSPT
SAYPERRGSPVPPVPERRSSPVPPVPERRGSLTLTISGESPKAGPAEEGP
SGPMEVLRKGSLRLRQLLSPKGERRMEDEGGFPVPQENGQPESPRRLSLG
QGDSTEAATEERGPRARLSSATANALYSSNLRDDTKAILEQISAHGQKHR
AVPAPSPGPTHNSPELGRPPAAGVLAPDMSDKDKCSAIFRSDSLGTQGRL
SRTLPASAEERDRLLRRMESMRKEKRVYSRFEVFCKKEEASSPGAGEGPA
EEGTRDSKVGKFVPKILGTFKSKK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU29641
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: silent mutations at Pro275 (CCG > CCA) and Ala425 (GCC > GCT)
• Low Temp: No