cDNA Clone ATG2B

• Plasmid: pBABED P GFP ATG2B
• Parent Plasmid: pBABED P GFP
• Expressed: GFP ATG2B
• Tag: GFP

Sequence of Insert

gtcgacATGCCTTGGCCGTTTTCGGAGTCCATCAAGAAGAGGGCCTGCCGGTACCTCCTGCAGAGGTACCTGGGCCACTTTCTGCAGGAGAAGCTGAGCCTGGAGCAGCTCAGCCTGGACCTGTACCAAGGCACCGGGTCCCTCGCCCAGGTCCCCTTGGACAAATGGTGTCTCAATGAGATCTTGGAGTCAGCAGATGCACCCTTAGAAGTCACTGAAGGATTCATTCAGTCAATTTCCCTGTCAGTTCCATGGGGCTCTTTACTGCAGGATAATTGTGCACTGGAAGTGAGAGGATTAGAAATGGTCTTCCGGCCTAGACCTCGCCCAGCAACTGGTTCTGAGCCTATGTATTGGTCAAGTTTTATGACCAGCAGTATGCAATTGGCAAAAGAATGTCTTAGCCAGAAACTAACAGATGAACAAGGAGAAGGATCCCAGCCTTTTGAAGGACTTGAAAAGTTTGCTGAAACCATTGAAACAGTACTAAGAAGAGTAAAAGTCACTTTTATAGATACTGTTTTGAGAATTGAACATGTGCCAGAAAATTCCAAAACTGGAACTGCACTTGAAATTCGAATAGAAAGAACTGTGTACTGTGATGAAACTGCTGACGAATCCTCAGGAATTAATGTGCATCAACCCACTGCTTTTGCTCACAAGTTACTTCAGCTCTCTGGAGTGTCTCTCTTCTGGGATGAGTTTTCTGCATCAGCCAAATCTTCCCCAGTGTGTTCAACTGCACCAGTGGAAACTGAGCCAAAGCTCTCACCTAGCTGGAACCCCAAAATTATTTATGAGCCACACCCACAGCTAACTAGAAATTTACCAGAGATAGCACCTTCTGACCCAGTGCAGATTGGACGGTTAATTGGTAGGTTGGAGTTGAGTCTCACGTTGAAACAGAATGAAGTGCTTCCTGGAGCTAAGTTGGATGTTGATGGACAGATAGACTCTATTCATCTACTCCTGTCACCAAGACAGGTGCACTTGCTTTTGGATATGTTGGCAGCTATTGCTGGACCAGAAAATTCTAGCAAAATAGGGTTAGCTAATAAAGATAGGAAAAATCGACCCATGCAGCAGGAAGACGAGTATCGAATTCAGATGGAATTAAACCGGTATTATTTGAGAAAAGATTCCCTCTCTGTGGGTGTATCTTCAGAGCAAAGCTTTTATGAGACAGAAACAGCTCGTACACCTTCTAGCCGTGAAGAAGAAGTATTCTTCTCCATGGCTGATATGGACATGTCTCATAGTCTCTCTTCTCTTCCACCCCTTGGGGACCCCCCAAACATGGACCTTGAGTTATCATTAACTAGTACATATACAAATACCCCAGCAGGATCTCCATTAAGTGCTACTGTGCTTCAGCCCACTTGGGGAGAGTTCCTTGATCATCATAAAGAACAGCCAGTAAGAGGGTCAACATTTCCATCCAACCTAGTTCACCCAACACCTTTACAGAAGACATCTCTCCCATCTAGATCTGTTTCAGTGGATGAATCAAGGCCTGAACTTATTTTTAGACTAGCTGTGGGAACTTTTTCAATCTCTGTGCTTCACATTGATCCTTTATCTCCACCTGAAACGTCACAGAACCTTAATCCATTGACACCTATGGCAGTAGCTTTCTTTACTTGTATAGAAAAGATTGATCCAGCAAGATTTTCAACAGAAGATTTTAAGTCTTTCCGAGCAGTGTTTGCAGAAGCTTGCTCACACGATCACCTTAGATTTATAGGTACTGGCATTAAAGTATCCTATGAACAAAGACAAAGATCAGCTTCCCGATATTTTAGTACTGATATGTCCATTGGGCAAATGGAATTTTTGGAGTGCCTATTTCCAACTGATTTTCATTCTGTTCCTCCTCACTATACAGAGCTTTTAACGTTCCATTCCAAAGAAGAAACTGGTTCCCATTCCCCTGTGTGTCTTCAGCTTCATTATAAGCATTCTGAGAATAGAGGGCCCCAGGGTAATCAAGCAAGACTTAGTTCAGTTCCTCACAAGGCAGAATTGCAAATTAAATTAAATCCAGTGTGTTGTGAGCTGGATATCAGTATTGTGGACAGGTTAAATTCCTTGCTTCAACCACAGAAACTTGCCACAGTAGAGATGATGGCATCCCACATGTATACTTCATATAATAAACATATTAGTCTGCACAAGGCTTTCACTGAAGTGTTTCTAGATGATTCACATAGTCCTGCAAATTGTCGGATATCAGTACAAGTTGCCACACCAGCATTAAACCTTTCTGTTCGCTTCCCAATACCTGATCTTCGATCTGATCAAGAAAGAGGACCATGGTTTAAGAAGTCACTTCAGAAGGAGATCCTTTATTTAGCCTTCACAGATCTAGAATTTAAGACTGAATTTATAGGAGGATCAACCCCAGAACAAATTAAATTGGAACTTACCTTTAGAGAACTAATTGGATCGTTCCAGGAAGAGAAAGGAGATCCATCTATTAAGTTTTTCCATGTGTCTAGTGGAGTAGATGGAGATACAACATCGTCAGATGACTTTGACTGGCCACGAATTGTACTGAAAATAAATCCACCAGCCATGCATTCCATTTTGGAGAGAATTGCAGCTGAAGAAGAAGAGGAGAATGATGGTCACTACCAGGAGGAAGAGGAAGGAGGTGCTCATTCCTTGAAAGATGTTTGTGATCTAAGAAGACCAGCCCCATCTCCTTTTTCTTCTCGTAGAGTAATGTTTGAAAATGAACAGATGGTGATGCCAGGAGACCCTGTAGAAATGACAGAATTTCAGGATAAAGCAATCAGCAATTCTCACTATGTGCTGGAACTTACGTTACCAAATATTTATGTAACACTACCTAATAAGAGCTTTTATGAGAAGCTTTATAATAGGATCTTTAATGACTTGCTACTGTGGGAACCAACAGCTCCTTCACCAGTGGAGACATTCGAGAATATTTCCTATGGCATTGGGCTTTCAGTAGCCAGTCAGCTCATTAATACTTTCAACAAAGATAGTTTTAGTGCATTTAAATCTGCAGTTCACTATGATGAGGAAAGTGGATCTGAGGAGGAGACTTTGCAGTATTTTTCCACTGTTGATCCCAACTATCGTTCTCGCAGGAAAAAAAAATTAGACTCTCAGAACAAGAACTCTCAGAGTTTTCTCTCAGTTCTTCTGAATATTAATCATGGATTAATAGCAGTGTTCACAGATGTGAAGCAAGATAATGGAGATCTGTTGGAAAACAAGCATGGTGAATTCTGGTTAGAGTTCAATAGTGGTTCATTATTTTGTGTGACAAAATATGAAGGTTTTGATGACAAGCACTACATTTGCCTTCATTCTAGCAGTTTCAGTCTGTACCATAAAGGCATAGTGAATGGAGTGATTCTCCCGACAGAAACACGACTTCCCAGCTCAACCCGCCCACACTGGTTGGAACCTACTATTTATTCCTCTGAAGAAGATGGCCTCAGTAAAACTTCTTCAGATGGAGTTGGAGGAGACAGTTTGAATATGCTGTCTGTTGCCGTTAAAATATTGTCTGATAAATCAGAGTCCAATACAAAGGAATTTCTCATTGCCGTAGGACTGAAAGGAGCCACTCTCCAGCATAGAATGCTTCCTTCTGGGCTTAGCTGGCATGAGCAGATTTTATACTTCTTGAATATTGCTGATGAACCTGTTTTGGGATATAATCCTCCAACTTCATTTACAACTTTTCATGTTCATCTTTGGAGCTGTGCACTTGATTATAGACCCCTTTATTTGCCAATCCGATCTCTTCTTACCGTGGAAACATTCAGTGTTTCCAGTAGCGTTGCATTGGATAAATCTTCCTCTACTCTCAGAATAATCTTGGATGAAGCTGCTTTACATCTATCTGACAAATGCAATACTGTCACTATAAATCTGAGTAGAGATTATGTTCGTGTGATGGATATGGGGCTTTTGGAGTTAACCATAACTGCAGTGAAGTCTGATTCTGATGGAGAGCAAACTGAGCCCCGCTTTGAGTTACACTGTTCCAGCGATGTTGTCCATATCAGAACGTGCTCAGACTCTTGTGCTGCGTTAATGAATCTCATTCAGTACATTGCAAGCTATGGTGACTTGCAGACACCTAACAAGGCAGATATGAAGCCTGGAGCCTTTCAAAGAAGGTCTAAGGTAGATTCCAGTGGTCGATCATCCTCACGTGGTCCAGTACTTCCTGAAGCAGATCAACAAATGTTACGAGATCTGATGAGTGATGCTATGGAGGAGATCGACATGCAACAAGGCACCTCGTCAGTAAAACCACAGGCTAATGGTGTTTTGGATGAAAAATCTCAAATTCAGGAGCCATGTTGTTCAGACCTCTTCCTGTTTCCTGACGAGAGTGGGAATGTATCCCAGGAGTCCGGCCCCACCTATGCCTCATTCTCTCACCATTTCATCAGTGATGCAATGACAGGTGTGCCCACTGAGAATGATGACTTTTGCATTCTTTTTGCACCAAAAGCAGCCATGCAGGAGAAGGAAGAAGAACCAGTTATAAAAATCATGGTTGATGATGCAATTGTGATAAGAGACAATTATTTCAGTCTGCCCGTTAATAAGACCGATACGAGCAAAGCCCCCTTACACTTTCCCATTCCTGTGATTCGCTATGTGGTGAAGGAGGTCTCTCTTGTCTGGCATCTTTATGGAGGAAAGGATTTTGGAACAGTCCCTCCCACTTCTCCGGCTAAAAGTTATATTAGTCCCCACAGTTCGCCTTCTCACACACCCACGAGACATGGACGTAATACAGTATGTGGGGGAAAAGGAAGGAACCATGACTTTTTAATGGAAATACAGCTAAGCAAGGTGAAGTTTCAGCATGAAGTCTACCCGCCATGCAAACCTGATTGTGATTCCAGCCTCTCAGAACACCCAGTCTCCCGGCAGGTGTTCATTGTTCAGGATCTTGAGATTCGAGATCGTTTGGCAACATCACAAATGAATAAATTTTTATACCTGTATTGCAGTAAAGAAATGCCTCGAAAAGCTCACTCCAACATGTTGACAGTGAAAGCCTTACACGTGTGTCCAGAATCTGGCAGGTCCCCACAGGAGTGCTGCTTGAGAGTGTCGCTGATGCCGCTCCGCCTCAATATTGACCAGGATGCTTTGTTCTTCCTGAAGGATTTCTTCACAAGTCTTTCTGCAGAAGTAGAGCTTCAAATGACTCCAGATCCAGAAGTTAAAAAGTCTCCTGGAGCTGATGTCACCTGCAGTTTGCCAAGGCATTTGAGTACCTCAAAGGAGCCAAATCTGGTTATTTCTTTCTCTGGGCCAAAACAGCCTTCCCAAAATGATAGTGCCAATTCAGTGGAAGTGGTTAATGGCATGGAAGAGAAGAACTTCTCTGCTGAAGAAGCATCTTTTAGGGATCAGCCTGTGTTTTTTAGAGAATTTAGATTCACGTCAGAAGTTCCCATTCGACTTGATTATCATGGCAAACATGTATCAATGGATCAGGGTACGCTAGCTGGGATTTTGATTGGTCTGGCTCAGTTAAACTGCTCTGAACTAAAGCTCAAGAGGCTTTCCTATCGACATGGTTTACTAGGCGTTGACAAATTATTCTCATATGCAATCACTGAGTGGCTTAATGACATTAAGAAGAACCAGCTACCAGGAATCCTGGGAGGTGTTGGACCTATGCATTCACTAGTACAATTAGTACAAGGCCTAAAGGACTTGGTCTGGCTCCCAATAGAGCAGTACCGGAAGGATGGCCGCATTGTCAGAGGGTTTCAGAGAGGCGCTGCTTCCTTTGGTACCTCGACAGCGATGGCTGCTCTAGAACTCACAAACAGAATGGTTCAAACCATACAGGCAGCTGCAGAGACTGCTTATGATATGGTGTCTCCTGGTACCCTTTCTATCGAGCCCAAGAAGACCAAAAGGTTTCCTCATCACCGGTTAGCCCACCAGCCAGTAGACCTGAGGGAAGGTGTGGCCAAGGCCTACAGTGTTGTGAAAGAGGGAATCACAGACACGGCTCAGACCATTTATGAAACTGCGGCTCGAGAACACGAGAGCAGAGGGGTGACTGGTGCCGTGGGCGAGGTTCTGCGCCAGATTCCTCCGGCAGTGGTGAAACCTCTGATTGTTGCCACAGAAGCAACGTCAAACGTGCTGGGTGGCATGAGAAACCAAATTAGGCCAGATGTCCGGCAAGACGAGTCACAGAAATGGCGCCACGGGGATGACTGAgcggccgc

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSSEFEVDMPWPFSESIKKRACRYLLQRYLGHFLQEKLSLEQLSLDLYQGTGSLAQVPLDKWCLNEILESADAPLEVTEGFIQSISLSVPWGSLLQDNCALEVRGLEMVFRPRPRPATGSEPMYWSSFMTSSMQLAKECLSQKLTDEQGEGSQPFEGLEKFAETIETVLRRVKVTFIDTVLRIEHVPENSKTGTALEIRIERTVYCDETADESSGINVHQPTAFAHKLLQLSGVSLFWDEFSASAKSSPVCSTAPVETEPKLSPSWNPKIIYEPHPQLTRNLPEIAPSDPVQIGRLIGRLELSLTLKQNEVLPGAKLDVDGQIDSIHLLLSPRQVHLLLDMLAAIAGPENSSKIGLANKDRKNRPMQQEDEYRIQMELNRYYLRKDSLSVGVSSEQSFYETETARTPSSREEEVFFSMADMDMSHSLSSLPPLGDPPNMDLELSLTSTYTNTPAGSPLSATVLQPTWGEFLDHHKEQPVRGSTFPSNLVHPTPLQKTSLPSRSVSVDESRPELIFRLAVGTFSISVLHIDPLSPPETSQNLNPLTPMAVAFFTCIEKIDPARFSTEDFKSFRAVFAEACSHDHLRFIGTGIKVSYEQRQRSASRYFSTDMSIGQMEFLECLFPTDFHSVPPHYTELLTFHSKEETGSHSPVCLQLHYKHSENRGPQGNQARLSSVPHKAELQIKLNPVCCELDISIVDRLNSLLQPQKLATVEMMASHMYTSYNKHISLHKAFTEVFLDDSHSPANCRISVQVATPALNLSVRFPIPDLRSDQERGPWFKKSLQKEILYLAFTDLEFKTEFIGGSTPEQIKLELTFRELIGSFQEEKGDPSIKFFHVSSGVDGDTTSSDDFDWPRIVLKINPPAMHSILERIAAEEEEENDGHYQEEEEGGAHSLKDVCDLRRPAPSPFSSRRVMFENEQMVMPGDPVEMTEFQDKAISNSHYVLELTLPNIYVTLPNKSFYEKLYNRIFNDLLLWEPTAPSPVETFENISYGIGLSVASQLINTFNKDSFSAFKSAVHYDEESGSEEETLQYFSTVDPNYRSRRKKKLDSQNKNSQSFLSVLLNINHGLIAVFTDVKQDNGDLLENKHGEFWLEFNSGSLFCVTKYEGFDDKHYICLHSSSFSLYHKGIVNGVILPTETRLPSSTRPHWLEPTIYSSEEDGLSKTSSDGVGGDSLNMLSVAVKILSDKSESNTKEFLIAVGLKGATLQHRMLPSGLSWHEQILYFLNIADEPVLGYNPPTSFTTFHVHLWSCALDYRPLYLPIRSLLTVETFSVSSSVALDKSSSTLRIILDEAALHLSDKCNTVTINLSRDYVRVMDMGLLELTITAVKSDSDGEQTEPRFELHCSSDVVHIRTCSDSCAALMNLIQYIASYGDLQTPNKADMKPGAFQRRSKVDSSGRSSSRGPVLPEADQQMLRDLMSDAMEEIDMQQGTSSVKPQANGVLDEKSQIQEPCCSDLFLFPDESGNVSQESGPTYASFSHHFISDAMTGVPTENDDFCILFAPKAAMQEKEEEPVIKIMVDDAIVIRDNYFSLPVNKTDTSKAPLHFPIPVIRYVVKEVSLVWHLYGGKDFGTVPPTSPAKSYISPHSSPSHTPTRHGRNTVCGGKGRNHDFLMEIQLSKVKFQHEVYPPCKPDCDSSLSEHPVSRQVFIVQDLEIRDRLATSQMNKFLYLYCSKEMPRKAHSNMLTVKALHVCPESGRSPQECCLRVSLMPLRLNIDQDALFFLKDFFTSLSAEVELQMTPDPEVKKSPGADVTCSLPRHLSTSKEPNLVISFSGPKQPSQNDSANSVEVVNGMEEKNFSAEEASFRDQPVFFREFRFTSEVPIRLDYHGKHVSMDQGTLAGILIGLAQLNCSELKLKRLSYRHGLLGVDKLFSYAITEWLNDIKKNQLPGILGGVGPMHSLVQLVQGLKDLVWLPIEQYRKDGRIVRGFQRGAASFGTSTAMAALELTNRMVQTIQAAAETAYDMVSPGTLSIEPKKTKRFPHHRLAHQPVDLREGVAKAYSVVKEGITDTAQTIYETAAREHESRGVTGAVGEVLRQIPPAVVKPLIVATEATSNVLGGMRNQIRPDVRQDESQKWRHGDD*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU59782
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Can you please clone ATG2B into pBabe puro vector?; GFP tag in the N-terminus please. Thank you!
• Low Temp: No