cDNA Clone CEP131

• Synonyms: AZ1; ZA1; AZI1
• Plasmid: pcDNA5 FRT/TO 3FLAG NLS CEP131
• Parent Plasmid: pcDNA5 FRT/TO FLAG1
• Expressed: 3FLAG NLS CEP131
• Genbank: NM_001319228.1
• Tag: 3FLAG
• Tag: NLS
• Species: Human

Amino Acid Sequence

MDYKDDDDKGSDYKDDDDKDYKDDDDKGPPKKKRKVMKGTRAIGSVPERSPAGVDLSLTGLPPPVSRRPGSAATTKPIVRSVSVVTGSEQKRKVLEATGP
GGSQAINNLRRSNSTTQVSQPRSGSPRPTEPTDFLMLFEGSPSGKKRPASLSTAPSEKGATWNVLDDQPRGFTLPSNARSSSALDSPAGPRRKECTVALA
PNFTANNRSNKGAVGNCVTTMVHNRYTPSERAPPLKSSNQTAPSLNNIIKAATCEGSESSGFGKLPKNVSSATHSARNNTGGSTGLPRRKEVTEEEAERF
IHQVNQAAVTIQRWYRHQVQRRGAGAARLEHLLQAKREEQRQRSGEGTLLDLHQQKEAARRKAREEKARQARRAAIQELQQKRALRAQKASTAERGPPEN
PRETRVPGMRQPAQELSPTPGGTAHQALKANNAGGGLPAAGPGDRCLPTSDSSPEPQQPPEDRTQDVLAQDAAGDNLEMMAPSRGSAKSRGPLEELLHTL
QLLEKEPDALPRPRTHHRGRYAWASEVTTEDDASSLTADNLEKFGKLSAFPEPPEDGTLLSEAKLQSIMSFLDEMEKSGQDQLDSQQEGWVPEAGPGPLE
LGSEVSTSVMRLKLEVEEKKQAMLLLQRALAQQRDLTARRVKETEKALSRQLQRQREHYEATIQRHLAFIDQLIEDKKVLSEKCEAVVAELKQEDQRCTE
RVAQAQAQHELEIKKLKELMSATEKARREKWISEKTKKIKEVTVRGLEPEIQKLIARHKQEVRRLKSLHEAELLQSDERASQRCLRQAEELREQLEREKE
ALGQQERERARQRFQQHLEQEQWALQQQRQRLYSEVAEERERLGQQAARQRAELEELRQQLEESSSALTRALRAEFEKGREEQERRHQMELNTLKQQLEL
ERQAWEAGRTRKEEAWLLNREQELREEIRKGRDKEIELVIHRLEADMALAKEESEKAAESRIKRLRDKYEAELSELEQSERKLQERCSELKGQLGEAEGE
NLRLQGLVRQKERALEDAQAVNEQLSSERSNLAQVIRQEFEDRLAASEEETRQAKAELATLQARQQLELEEVHRRVKTALARKEEAVSSLRTQHEAAVKR
ADHLEELLEQHRRPTPSTK*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pcDNA5 FRT/TO 3FLAG NLS CEP13174.52 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU61027
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Compared to reference sequence there are 3 SNP at T272A, T397A and V473A. There are also two silent mutations at T383 (a1149g) and A611 (91833a). Original clone used to make this would have been BglII - Not1 into pcDNA5 FRT/TO FLAG1 BamH1 - Not1 (BglII/BamH1 site killed) - see attached SnapGene file.
• Low Temp: No