Plasmid
pBABE puro HA ATG2B S495A
Parent Plasmid
pBABE puro HA
Expressed
HA ATG2B S495A
Tag
HA
Sequence of Insert
View Sequence
GTCGACATGCCTTGGCCGTTTTCGGAGTCCATCAAGAAGAGGGCCTGCCGGTACCTCCTGCAGAGGTACCTGGGCCACTTTCTGCAGGAGAAGCTGAGCCTGGAGCAGCTCAGCCTGGACCTGTACCAAGGCACCGGGTCCCTCGCCCAGGTCCCCTTGGACAAATGGTGTCTCAATGAGATCTTGGAGTCAGCAGATGCACCCTTAGAAGTCACTGAAGGATTCATTCAGTCAATTTCCCTGTCAGTTCCATGGGGCTCTTTACTGCAGGATAATTGTGCACTGGAAGTGAGAGGATTAGAAATGGTCTTCCGGCCTAGACCTCGCCCAGCAACTGGTTCTGAGCCTATGTATTGGTCAAGTTTTATGACCAGCAGTATGCAATTGGCAAAAGAATGTCTTAGCCAGAAACTAACAGATGAACAAGGAGAAGGATCCCAGCCTTTTGAAGGACTTGAAAAGTTTGCTGAAACCATTGAAACAGTACTAAGAAGAGTAAAAGTCACTTTTATAGATACTGTTTTGAGAATTGAACATGTGCCAGAAAATTCCAAAACTGGAACTGCACTTGAAATTCGAATAGAAAGAACTGTGTACTGTGATGAAACTGCTGACGAATCCTCAGGAATTAATGTGCATCAACCCACTGCTTTTGCTCACAAGTTACTTCAGCTCTCTGGAGTGTCTCTCTTCTGGGATGAGTTTTCTGCATCAGCCAAATCTTCCCCAGTGTGTTCAACTGCACCAGTGGAAACTGAGCCAAAGCTCTCACCTAGCTGGAACCCCAAAATTATTTATGAGCCACACCCACAGCTAACTAGAAATTTACCAGAGATAGCACCTTCTGACCCAGTGCAGATTGGACGGTTAATTGGTAGGTTGGAGTTGAGTCTCACGTTGAAACAGAATGAAGTGCTTCCTGGAGCTAAGTTGGATGTTGATGGACAGATAGACTCTATTCATCTACTCCTGTCACCAAGACAGGTGCACTTGCTTTTGGATATGTTGGCAGCTATTGCTGGACCAGAAAATTCTAGCAAAATAGGGTTAGCTAATAAAGATAGGAAAAATCGACCCATGCAGCAGGAAGACGAGTATCGAATTCAGATGGAATTAAACCGGTATTATTTGAGAAAAGATTCCCTCTCTGTGGGTGTATCTTCAGAGCAAAGCTTTTATGAGACAGAAACAGCTCGTACACCTTCTAGCCGTGAAGAAGAAGTATTCTTCTCCATGGCTGATATGGACATGTCTCATAGTCTCTCTTCTCTTCCACCCCTTGGGGACCCCCCAAACATGGACCTTGAGTTATCATTAACTAGTACATATACAAATACCCCAGCAGGATCTCCATTAAGTGCTACTGTGCTTCAGCCCACTTGGGGAGAGTTCCTTGATCATCATAAAGAACAGCCAGTAAGAGGGTCAACATTTCCATCCAACCTAGTTCACCCAACACCTTTACAGAAGACATCTCTCCCATCTAGATCTGTTTCAGTGGATGAATCAAGGCCTGAACTTATTTTTAGACTAGCTGTGGGAACTTTTTCAATCTCTGTGCTTCACATTGATCCTTTATCTCCACCTGAAACGTCACAGAACCTTAATCCATTGACACCTATGGCAGTAGCTTTCTTTACTTGTATAGAAAAGATTGATCCAGCAAGATTTTCAACAGAAGATTTTAAGTCTTTCCGAGCAGTGTTTGCAGAAGCTTGCTCACATGATCACCTTAGATTTATAGGTACTGGCATTAAAGTATCCTATGAACAAAGACAAAGATCAGCTTCCCGATATTTTAGTACTGATATGTCCATTGGGCAAATGGAATTTTTGGAGTGCCTATTTCCAACTGATTTTCATTCTGTTCCTCCTCACTATACAGAGCTTTTAACGTTCCATTCCAAAGAAGAAACTGGTTCCCATTCCCCTGTGTGTCTTCAGCTTCATTATAAGCATTCTGAGAATAGAGGGCCCCAGGGTAATCAAGCAAGACTTAGTTCAGTTCCTCACAAGGCAGAATTGCAAATTAAATTAAATCCAGTGTGTTGTGAGCTGGATATCAGTATTGTGGACAGGTTAAATTCCTTGCTTCAACCACAGAAACTTGCCACAGTAGAGATGATGGCATCCCACATGTATACTTCATATAATAAACATATTAGTCTGCACAAGGCTTTCACTGAAGTGTTTCTAGATGATTCACATAGTCCTGCAAATTGTCGGATATCAGTACAAGTTGCCACACCAGCATTAAACCTTTCTGTTCGCTTCCCAATACCTGATCTTCGATCTGATCAAGAAAGAGGACCATGGTTTAAGAAGTCACTTCAGAAGGAGATCCTTTATTTAGCCTTCACAGATCTAGAATTTAAGACTGAATTTATAGGAGGATCAACCCCAGAACAAATTAAATTGGAACTTACCTTTAGAGAACTAATTGGATCGTTCCAGGAAGAGAAAGGAGATCCATCTATTAAGTTTTTCCATGTGTCTAGTGGAGTAGATGGAGATACAACATCGTCAGATGACTTTGACTGGCCACGAATTGTACTGAAAATAAATCCACCAGCCATGCATTCCATTTTGGAGAGAATTGCAGCTGAAGAAGAAGAGGAGAATGATGGTCACTACCAGGAGGAAGAGGAAGGAGGTGCTCATTCCTTGAAAGATGTTTGTGATCTAAGAAGACCAGCCCCATCTCCTTTTTCTTCTCGTAGAGTAATGTTTGAAAATGAACAGATGGTGATGCCAGGAGACCCTGTAGAAATGACAGAATTTCAGGATAAAGCAATCAGCAATTCTCACTATGTGCTGGAACTTACGTTACCAAATATTTATGTAACACTACCTAATAAGAGCTTTTATGAGAAGCTTTATAATAGGATCTTTAATGACTTGCTACTGTGGGAACCAACAGCTCCTTCACCAGTGGAGACATTCGAGAATATTTCCTATGGCATTGGGCTTTCAGTAGCCAGTCAGCTCATTAATACTTTCAACAAAGATAGTTTTAGTGCATTTAAATCTGCAGTTCACTATGATGAGGAAAGTGGATCTGAGGAGGAGACTTTGCAGTATTTTTCCACTGTTGATCCCAACTATCGTTCTCGCAGGAAAAAAAAATTAGACTCTCAGAACAAGAACTCTCAGAGTTTTCTCTCAGTTCTTCTGAATATTAATCATGGATTAATAGCAGTGTTCACAGATGTGAAGCAAGATAATGGAGATCTGTTGGAAAACAAGCATGGTGAATTCTGGTTAGAGTTCAATAGTGGTTCATTATTTTGTGTGACAAAATATGAAGGTTTTGATGACAAGCACTACATTTGCCTTCATTCTAGCAGTTTCAGTCTGTACCATAAAGGCATAGTGGATGGAGTGATTCTCCCGACAGAAACACGACTTCCCAGCTCAACCCGCCCACACTGGTTGGAACCTACTATTTATTCCTCTGAAGAAGATGGCCTCAGTAAAACTTCTTCAGATGGAGTTGGAGGAGACAGTTTGAATATGCTGTCTGTTGCCGTTAAAATATTGTCTGATAAATCAGAGTCCAATACAAAGGAATTTCTCATTGCCGTAGGACTGAAAGGAGCCACTCTCCAGCATAGAATGCTTCCTTCTGGGCTTAGCTGGCATGAGCAGATTTTATACTTCTTGAATATTGCTGATGAACCTGTTTTGGGATATAATCCTCCAACTTCATTTACAACTTTTCATGTTCATCTTTGGAGCTGTGCACTTGATTATAGACCCCTTTATTTGCCAATCCGATCTCTTCTTACCGTGGAAACATTCAGTGTTTCCAGTAGCGTTGCATTGGATAAATCTTCCTCTACTCTCAGAATAATCTTGGATGAAGCTGCTTTACATCTATCTGACAAATGCAATACTGTCACTATAAATCTGAGTAGAGATTATGTTCGTGTGATGGATATGGGGCTTTTGGAGTTAACCATAACTGCAGTGAAGTCTGATTCTGATGGAGAGCAAACTGAGCCCCGCTTTGAGTTACACTGTTCCAGCGATGTTGTCCATATCAGAACGTGCTCAGACTCTTGTGCTGCGTTAATGAATCTCATTCAGTACATTGCAAGCTATGGTGACTTGCAGACACCTAACAAGGCAGATATGAAGCCTGGAGCCTTTCAAAGAAGGTCTAAGGTAGATTCCAGTGGTCGATCATCCTCACGTGGTCCAGTACTTCCTGAAGCAGATCAACAAATGTTACGAGATCTGATGAGTGATGCTATGGAGGAGATCGACATGCAACAAGGCACCTCGTCAGTAAAACCACAGGCTAATGGTGTTTTGGATGAAAAATCTCAAATTCAGGAGCCATGTTGTTCAGACCTCTTCCTGTTTCCTGACGAGAGTGGGAATGTATCCCAGGAGTCCGGCCCCACCTATGCCTCATTCTCTCACCATTTCATCAGTGATGCAATGACAGGTGTGCCCACTGAGAATGATGACTTTTGCATTCTTTTTGCACCAAAAGCAGCCATGCAGGAGAAGGAAGAAGAACCAGTTATAAAAATCATGGTTGATGATGCAATTGTGATAAGAGACAATTATTTCAGTCTGCCCGTTAATAAGACCGATACGAGCAAAGCCCCCTTACACTTTCCCATTCCTGTGATTCGCTATGTGGTGAAGGAGGTCTCTCTTGTCTGGCATCTTTATGGAGGAAAGGATTTTGGAACAGTCCCTCCCACTTCTCCGGCTAAAAGTTATATTAGTCCCCACAGTTCGCCTTCTCACACACCCACGAGACATGGACGTAATACAGTATGTGGGGGAAAAGGAAGGAACCATGACTTTTTAATGGAAATACAGCTAAGCAAGGTGAAGTTTCAGCATGAAGTCTACCCGCCATGCAAACCTGATTGTGATTCCAGCCTCTCAGAACACCCAGTCTCCCGGCAGGTGTTCATTGTTCAGGATCTTGAGATTCGAGATCGTTTGGCAACATCACAAATGAATAAATTTTTATACCTGTATTGCAGTAAAGAAATGCCTCGAAAAGCTCACTCCAACATGTTGACAGTGAAAGCCTTACACGTGTGTCCAGAATCTGGCAGGTCCCCACAGGAGTGCTGCTTGAGAGTGTCGCTGATGCCGCTCCGCCTCAATATTGACCAGGATGCTTTGTTCTTCCTGAAGGATTTCTTCACAAGTCTTTCTGCAGAAGTAGAGCTTCAAATGACTCCAGATCCAGAAGTTAAAAAGTCTCCTGGAGCTGATGTCACCTGCAGTTTGCCAAGGCATTTGAGTACCTCAAAGGAGCCAAATCTGGTTATTTCTTTCTCTGGGCCAAAACAGCCTTCCCAAAATGATAGTGCCAATTCAGTGGAAGTGGTTAATGGCATGGAAGAGAAGAACTTCTCTGCTGAAGAAGCATCTTTTAGGGATCAGCCTGTGTTTTTTAGAGAATTTAGATTCACGTCAGAAGTTCCCATTCGACTTGATTATCATGGCAAACATGTATCAATGGATCAGGGTACGCTAGCTGGGATTTTGATTGGTCTGGCTCAGTTAAACTGCTCTGAACTAAAGCTCAAGAGGCTTTCCTATCGACATGGTTTACTAGGCGTTGACAAATTATTCTCATATGCAATCACTGAGTGGCTTAATGACATTAAGAAGAACCAGCTACCAGGAATCCTGGGAGGTGTTGGACCTATGCATTCACTAGTACAATTAGTACAAGGCCTAAAGGACTTGGTCTGGCTCCCAATAGAGCAGTACCGGAAGGATGGCCGCATTGTCAGAGGGTTTCAGAGAGGCGCTGCTTCCTTTGGTACCTCGACAGCGATGGCTGCTCTAGAACTCACAAACAGAATGGTTCAAACCATACAGGCAGCTGCAGAGACTGCTTATGATATGGTGTCTCCTGGTACCCTTTCTATCGAGCCCAAGAAGACCAAAAGGTTTCCTCATCACCGGTTAGCCCACCAGCCAGTAGACCTGAGGGAAGGTGTGGCCAAGGCCTACAGTGTTGTGAAAGAGGGAATCACAGACACGGCTCAGACCATTTATGAAACTGCGGCTCGAGAACACGAGAGCAGAGGGGTGACTGGTGCCGTGGGCGAGGTTCTGCGCCAGATTCCTCCGGCAGTGGTGAAACCTCTGATTGTTGCCACAGAAGCAACGTCAAACGTGCTGGGTGGCATGAGAAACCAAATTAGGCCAGATGTCCGGCAAGACGAGTCACAGAAATGGCGCCACGGGGATGACTGAGCGGCCGC
Amino Acid Sequence
View Sequence
MYPYDVPDYAGSSEFEVDMPWPFSESIKKRACRYLLQRYLGHFLQEKLSLEQLSLDLYQGTGSLAQVPLDKWCLNEILESADAPLEVTEGFIQSISLSVPWGSLLQDNCALEVRGLEMVFRPRPRPATGSEPMYWSSFMTSSMQLAKECLSQKLTDEQGEGSQPFEGLEKFAETIETVLRRVKVTFIDTVLRIEHVPENSKTGTALEIRIERTVYCDETADESSGINVHQPTAFAHKLLQLSGVSLFWDEFSASAKSSPVCSTAPVETEPKLSPSWNPKIIYEPHPQLTRNLPEIAPSDPVQIGRLIGRLELSLTLKQNEVLPGAKLDVDGQIDSIHLLLSPRQVHLLLDMLAAIAGPENSSKIGLANKDRKNRPMQQEDEYRIQMELNRYYLRKDSLSVGVSSEQSFYETETARTPSSREEEVFFSMADMDMSHSLSSLPPLGDPPNMDLELSLTSTYTNTPAGSPLSATVLQPTWGEFLDHHKEQPVRGSTFPSNLVHPTPLQKTSLPSRSVSVDESRPELIFRLAVGTFSISVLHIDPLSPPETSQNLNPLTPMAVAFFTCIEKIDPARFSTEDFKSFRAVFAEACSHDHLRFIGTGIKVSYEQRQRSASRYFSTDMSIGQMEFLECLFPTDFHSVPPHYTELLTFHSKEETGSHSPVCLQLHYKHSENRGPQGNQARLSSVPHKAELQIKLNPVCCELDISIVDRLNSLLQPQKLATVEMMASHMYTSYNKHISLHKAFTEVFLDDSHSPANCRISVQVATPALNLSVRFPIPDLRSDQERGPWFKKSLQKEILYLAFTDLEFKTEFIGGSTPEQIKLELTFRELIGSFQEEKGDPSIKFFHVSSGVDGDTTSSDDFDWPRIVLKINPPAMHSILERIAAEEEEENDGHYQEEEEGGAHSLKDVCDLRRPAPSPFSSRRVMFENEQMVMPGDPVEMTEFQDKAISNSHYVLELTLPNIYVTLPNKSFYEKLYNRIFNDLLLWEPTAPSPVETFENISYGIGLSVASQLINTFNKDSFSAFKSAVHYDEESGSEEETLQYFSTVDPNYRSRRKKKLDSQNKNSQSFLSVLLNINHGLIAVFTDVKQDNGDLLENKHGEFWLEFNSGSLFCVTKYEGFDDKHYICLHSSSFSLYHKGIVDGVILPTETRLPSSTRPHWLEPTIYSSEEDGLSKTSSDGVGGDSLNMLSVAVKILSDKSESNTKEFLIAVGLKGATLQHRMLPSGLSWHEQILYFLNIADEPVLGYNPPTSFTTFHVHLWSCALDYRPLYLPIRSLLTVETFSVSSSVALDKSSSTLRIILDEAALHLSDKCNTVTINLSRDYVRVMDMGLLELTITAVKSDSDGEQTEPRFELHCSSDVVHIRTCSDSCAALMNLIQYIASYGDLQTPNKADMKPGAFQRRSKVDSSGRSSSRGPVLPEADQQMLRDLMSDAMEEIDMQQGTSSVKPQANGVLDEKSQIQEPCCSDLFLFPDESGNVSQESGPTYASFSHHFISDAMTGVPTENDDFCILFAPKAAMQEKEEEPVIKIMVDDAIVIRDNYFSLPVNKTDTSKAPLHFPIPVIRYVVKEVSLVWHLYGGKDFGTVPPTSPAKSYISPHSSPSHTPTRHGRNTVCGGKGRNHDFLMEIQLSKVKFQHEVYPPCKPDCDSSLSEHPVSRQVFIVQDLEIRDRLATSQMNKFLYLYCSKEMPRKAHSNMLTVKALHVCPESGRSPQECCLRVSLMPLRLNIDQDALFFLKDFFTSLSAEVELQMTPDPEVKKSPGADVTCSLPRHLSTSKEPNLVISFSGPKQPSQNDSANSVEVVNGMEEKNFSAEEASFRDQPVFFREFRFTSEVPIRLDYHGKHVSMDQGTLAGILIGLAQLNCSELKLKRLSYRHGLLGVDKLFSYAITEWLNDIKKNQLPGILGGVGPMHSLVQLVQGLKDLVWLPIEQYRKDGRIVRGFQRGAASFGTSTAMAALELTNRMVQTIQAAAETAYDMVSPGTLSIEPKKTKRFPHHRLAHQPVDLREGVAKAYSVVKEGITDTAQTIYETAAREHESRGVTGAVGEVLRQIPPAVVKPLIVATEATSNVLGGMRNQIRPDVRQDESQKWRHGDD*
Vector Type
Mammalian
Antibiotic
Amp
DU Number
DU59711
Unit Source
Location
PPU
MTA
Notes
silent change C1719T, difference to reference sequence N1124D
Low Temp
No
Price
£125.00
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