cDNA Clone MINDY2 (KIAA1164)

• Plasmid: pGEX6P1 KIAA1164 C266A Y319A M323A R342A E350A Y351A D359A E392A 241-504
• Parent Plasmid: pGEX6P1
• Expressed: GST KIAA1164 C266A Y319A M323A R342A E350A Y351A D359A E392A 241-504
• Tag: GST

Sequence of Insert

AGATCTATGTCTGTGTATCACATCAAGTGGATCCAGTGGAAGGAAGAGAACACACCCATCATCACCCAGAATGAGAACGGACCCGCTCCCTTGCTGGCCATCCTCAATGTTTTGCTCCTGGCCTGGAAGGTGAAACTTCCACCGATGATGGAAATCATAACTGCTGAGCAGCTGATGGAATATTTAGGAGATTACATGCTTGATGCAAAGCCAAAAGAAATTTCAGAAATTCAACGTTTAAATGCTGAACAGAATGCGAGTGATGCCATGGCAATTTTGCACAAACTACAGACAGGCCTGGATGTAAATGTAGCATTCACTGGTGTTCGAGTGTTTGCAGCTACACCAGAATGCATAGTATTTGCTCTTCTTGATATTCCTTTGTACCATGGGTGGTTAGTAGACCCTCAGATTGATGACATTGTAAAAGCTGTTGGTAACTGCAGCTACAACCAACTAGTGGCGAAGATCATCTCTTGTAAACAGTCAGACAATAGTGAGCTGGTTAGTGAAGGCTTTGTAGCTGAGCAGTTTCTAAATAACACAGCCACTCAACTGACATACCATGGATTATGTGAACTAACTTCAACGGTTCAGGAAGGAGAACTTTGTGTGTTCTTTCGGAATAATCATTTTAGCACCATGACCAAATACAAGGGTCAACTGTATTTGTTGGTAACGGACCAGGGGTTTCTTACTGAAGAGAAAGTTGTTTGGGAAAGCCTACACAACGTAGATGGTGATGGAAATTTCTGTGACTCAGAATTTCATCTTCGACCTCCTTCAGATCCTGAAACTGTATAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMSVYHIKWIQWKEENTPIITQNENGPAPLLAILNVLLLAWKVKLPPMMEIITAEQLMEYLGDYMLDAKPKEISEIQRLNAEQNASDAMAILHKLQTGLDVNVAFTGVRVFAATPECIVFALLDIPLYHGWLVDPQIDDIVKAVGNCSYNQLVAKIISCKQSDNSELVSEGFVAEQFLNNTATQLTYHGLCELTSTVQEGELCVFFRNNHFSTMTKYKGQLYLLVTDQGFLTEEKVVWESLHNVDGDGNFCDSEFHLRPPSDPETV*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU59639
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Please mutate C266 to alanine in 59473
• Low Temp: No