cDNA Clone ATG2A

• Plasmid: pBABE hygro myc ATG2A
• Parent Plasmid: pBABE hygro myc
• Expressed: MYC ATG2A
• Tag: Myc

Sequence of Insert

GGATCCATGTCACGATGGCTGTGGCCATGGTCAAACTGTGTGAAAGAGCGGGTCTGCCGCTACTTGCTGCACCACTACTTAGGTCACTTCTTCCAAGAGCACCTCAGCCTGGACCAGCTCAGCCTCGATCTGTACAAGGGCAGCGTTGCCCTGCGAGACATCCACCTGGAAATCTGGTCTGTGAACGAGGTGCTGGAGTCAATGGAGTCACCGCTGGAGCTGGTGGAAGGCTTCGTGGGCTCCATCGAGGTGGCCGTGCCCTGGGCTGCTCTGCTCACCGACCACTGCACAGTGCGCGTGTCCGGCCTCCAGCTCACCTTGCAGCCCCGCCGGGGTCCAGCGCCAGGGGCTGCCGACTCACAGAGCTGGGCCTCATGCATGACCACAAGCCTGCAGCTGGCCCAGGAGTGTCTGCGGGATGGGCTACCGGAGCCCTCTGAGCCACCACAGCCCCTGGAGGGGCTGGAGATGTTTGCCCAGACCATTGAGACTGTGCTTCGGAGGATCAAAGTGACCTTCCTGGACACTGTCGTGAGGGTGGAGCACTCTCCGGGTGATGGGGAACGTGGTGTGGCCGTCGAGGTCCGTGTGCAGAGACTGGAGTACTGTGATGAGGCAGTGCGGGACCCAAGCCAGGCGCCGCCGGTGGATGTGCATCAGCCGCCTGCCTTCCTGCACAAGCTGCTGCAGCTGGCAGGGGTCCGCCTGCACTACGAGGAGCTCCCGGCACAGGAAGAGCCTCCAGAGCCCCCCTTGCAGATCGGCAGCTGCTCAGGGTACATGGAGCTGATGGTGAAGTTGAAGCAAAATGAGGCCTTCCCTGGCCCCAAGTTGGAGGTGGCGGGACAGCTGGGCTCCCTGCACCTGCTCCTGACCCCGAGGCAGCTCCAGCAACTTCAGGAACTGCTCAGCGCCGTGAGCCTTACAGACCACGAGGGCCTGGCTGACAAGCTGAACAAGAGCCGCCCGCTAGGTGCCGAAGACCTGTGGCTGATTGAGCAGGACCTGAACCAGCAGCTGCAGGCAGGGGCAGTGGCTGAGCCCCTCAGCCCAGACCCCCTTACCAACCCCCTTCTCAACCTGGATAACACTGACCTCTTCTTCTCCATGGCTGGCCTCACAAGCAGTGTGGCCTCAGCCCTCTCTGAGCTCTCCCTCTCCGATGTAGACCTGGCCTCCTCTGTGCGCAGCGACATGGCCTCCCGCCGGCTCTCTGCCCAGGCCCACCCAGCTGGCAAGATGGCCCCCAACCCCCTCCTGGACACCATGCGCCCTGACTCGCTGCTGAAGATGACCTTGGGGGGTGTGACCCTGACCTTGCTTCAGACGTCTGCCCCATCTTCCGGACCACCTGACCTCGCCACGCACTTTTTCACCGAGTTTGATGCCACCAAGGATGGGCCCTTCGGTTCCCGAGACTTCCATCACCTTCGACCACGCTTCCAGAGGGCCTGTCCCTGTAGCCATGTTCGGCTAACGGGCACAGCCGTGCAGCTGTCCTGGGAGCTGCGGACGGGCAGTCGGGGCCGGCGGACAACCAGCATGGAAGTGCACTTCGGGCAGCTGGAGGTGCTGGAGTGTCTGTGGCCCCGGGGCACCTCTGAGCCTGAGTACACGGAGATCCTGACCTTTCCTGGTACGCTGGGCTCCCAGGCCTCAGCTCGGCCCTGCGCCCATCTGCGCCACACACAGATCCTGCGCCGTGTGCCTAAGAGCCGACCCCGGCGCTCAGTTGCCTGCCATTGCCACTCAGAACTGGCCCTGGACCTGGCCAACTTCCAGGCGGACGTGGAGCTGGGGGCCCTGGACCGGCTGGCCGCCCTACTGCGCCTGGCCACCGTACCTGCTGAGCCTCCAGCCGGCCTGCTGACAGAGCCCCTGCCGGCGATGGAGCAGCAGACGGTATTTCGGCTCTCTGCACCCCGGGCCACGCTGCGGCTGCGCTTCCCCATTGCCGACCTGCGGCCTGAGCGGGACCCCTGGGCGGGCCAGGCCGTGCGGGCTGAGCAGCTTCGGCTGGAGCTGAGTGAGCCCCAGTTCCGGTCAGAGCTTAGCAGTGGGCCTGGTCCCCCAGTCCCCACCCACCTGGAACTCACCTGCTCCGACCTACATGGTATCTATGAAGATGGAGGGAAGCCACCTGTCCCTTGCCTGCGTGTCTCCAAAGCCCTGGACCCCAAGAGCACTGGGCGCAAGTACTTCCTGCCCCAGGTAGTGGTGACTGTGAACCCCCAGTCCAGCAGCACACAGTGGGAGGTGGCCCCGGAGAAGGGAGAGGAACTGGAGCTGTCAGTGGAGAGTCCCTGTGAGCTGCGGGAACCTGAGCCCTCGCCCTTCTCCTCTAAGAGGACCATGTATGAGACAGAGGAGATGGTGATCCCTGGAGACCCTGAGGAGATGAGGACGTTCCAGAGCCGGACCCTGGCACTGTCCCGCTGCAGCCTGGAAGTGATCCTGCCCAGTGTCCACATCTTTCTGCCCAGCAAGGAGGTCTACGAGAGCATCTACAACAGGATCAACAACGACCTGCTCATGTGGGAGCCTGCAGATCTGCTTCCCACCCCCGACCCCGCCGCCCAGCCCTCGGGCTTCCCCGGCCCCTCAGGCTTCTGGCACGACAGCTTTAAGATGTGCAAGTCAGCCTTCAAGCTGGCCAACTGCTTTGATCTCACCCCAGACTCGGACTCGGATGACGAGGATGCCCACTTCTTCTCAGTGGGGGCATCAGGTGGCCCACAGGCCGCTGCCCCTGAGGCCCCAAGTCTTCACTTGCAGAGCACCTTCTCTACACTGGTGACAGTGCTGAAGGGGCGGATCACAGCCCTCTGTGAGACCAAGGATGAGGGTGGGAAGCGGCTGGAGGCTGTGCACGGGGAGCTGGTGCTGGACATGGAGCACGGTACCCTCTTCAGCGTCTCCCAGTACTGTGGCCAGCCAGGACTTGGCTACTTCTGTCTGGAAGCTGAAAAGGCAACACTCTACCACCGAGCGGCCGTGGATGACTACCCGCTGCCCAGTCACCTGGACCTTCCCAGTTTCGCTCCCCCGGCTCAGCTGGCCCCAACCATCTACCCATCGGAGGAAGGGGTGACCGAGCGGGGAGCCTCGGGCCGCAAGGGCCAGGGCCGGGGACCCCACATGTTGTCCACTGCTGTGCGCATCCACCTGGACCCCCACAAGAATGTGAAGGAGTTCCTGGTGACACTGCGGTTGCACAAAGCCACCTTGCGCCACTACATGGCCCTGCCCGAGCAGAGCTGGCATTCCCAGTTGTTGGAGTTCCTAGACGTGCTGGATGACCCTGTGCTGGGCTACCTGCCCCCGACGGTCATCACCATCCTGCACACACACCTGTTCTCCTGCTCTGTGGACTATAGGCCACTCTACCTCCCAGTGCGTGTCCTCATCACCGCGGAGACCTTCACTCTCTCCAGCAACATCATCATGGACACCTCCACCTTCCTGCTCAGGTTCATCCTCGATGACTCCGCCTTGTACCTGTCCGACAAGTGTGAGGTGGAGACCCTGGACCTGCGGCGAGATTATGTCTGTGTTTTGGATGTTGACCTCTTGGAACTTGTGATTAAAACCTGGAAAGGGAGCACCGAGGGCAAACTGAGCCAGCCACTATTCGAGCTGCGCTGCTCCAACAATGTGGTACACGTGCACAGCTGTGCCGACTCCTGTGCCCTGCTGGTCAACCTGCTCCAGTACGTAATGAGCACAGGCGATCTGCACCCCCCACCCCGGCCCCCCAGCCCCACGGAGATCGCCGGCCAGAAGCTCTCGGAGAGTCCTGCCTCTCTGCCCTCGTGCCCCCCAGTGGAGACGGCCCTCATCAACCAGCGTGACCTGGCCGACGCCCTCCTGGACACCGAGCGCAGCCTACGGGAGCTGGCCCAGCCTTCAGGTGGCCACCTCCCTCAGGCGTCGCCCATCTCCGTCTACCTATTCCCAGGTGAACGGAGTGGGGCCCCACCCCCTTCACCACCTGTCGGGGGCCCTGCTGGCAGCTTAGGGTCATGCTCAGAGGAGAAGGAAGATGAAAGGGAAGAGGAGGGCGATGGAGACACCCTGGACAGTGATGAGTTCTGCATCCTTGATGCTCCCGGCCTGGGCATCCCGCCCCGAGATGGGGAGCCTGTGGTGACACAGCTGCATCCCGGCCCCATCGTTGTGAGGGACGGTTACTTCTCACGGCCGATCGGCAGCACGGACTTGCTGCGGGCACCTGCCCATTTCCCAGTGCCCAGCACTCGGGTGGTGCTACGTGAGGTCTCCCTCGTCTGGCACCTCTATGGGGGCCGAGACTTTGGCCCCCACCCCGGCCACAGGGCAAGAACTGGCCTCTCAGGTCCCAGGAGCTCCCCTTCCCGCTGCTCTGGCCCCAACCGGCCCCAGAACTCATGGCGCACGCAGGGGGGCAGCGGGCGGCAGCACCATGTCCTCATGGAGATCCAGCTGAGCAAGGTAAGCTTCCAGCACGAGGTGTACCCAGCGGAGCCAGCCACAGGCCCTGCGGCCCCCAGCCAGGAGCTGGAGGAGCGACCGCTGTCCCGTCAGGTGTTCATCGTGCAGGAGCTGGAGGTCCGAGACCGGCTCGCCTCCTCCCAGATCAACAAGTTCCTGTACCTACACACGAGTGAGCGGATGCCGCGACGTGCCCACTCTAACATGCTCACCATCAAAGCGCTGCATGTGGCCCCCACTACCAACCTGGGTGGGCCTGAGTGCTGTCTCCGCGTCTCGCTGATGCCCCTGCGGCTCAATGTGGACCAGGATGCCCTCTTCTTCCTCAAGGACTTCTTCACTAGTCTGGTGGCCGGCATCAACCCCGTGGTCCCAGGGGAGACCTCCGCTGAGGCTCGCCCCGAGACTCGAGCCCAGCCCAGCAGCCCCCTGGAAGGGCAGGCCGAAGGCGTAGAGACCACTGGTTCGCAGGAGGCCCCAGGAGGTGGACACAGCCCCTCCCCTCCTGACCAGCAGCCCATCTACTTCAGAGAGTTCCGCTTCACGTCTGAGGTCCCCATCTGGCTGGATTACCATGGCAAGCACGTCACGATGGACCAGGTGGGCACTTTTGCTGGCCTCCTCATCGGCCTGGCCCAACTCAACTGCTCCGAGCTGAAGCTAAAGCGGCTCTGTTGCAGGCACGGGCTCCTGGGTGTGGACAAGGTGCTGGGCTATGCCCTCAACGAGTGGCTGCAGGACATCCGCAAGAACCAGCTGCCCGGCCTGCTGGGAGGCGTGGGCCCCATGCACTCGGTTGTCCAGCTCTTCCAAGGGTTCCGGGACCTGCTGTGGCTGCCCATTGAGCAGTACAGGAAGGATGGCCGCCTCATGCGGGGGCTGCAGCGAGGGGCTGCCTCCTTTGGCTCATCCACAGCCTCTGCCGCCCTGGAACTCAGCAACCGGTTGGTACAGGCTATCCAGGCCACAGCTGAGACCGTGTATGACATCCTGTCCCCGGCAGCCCCCGTCTCCCGCTCCCTGCAGGATAAGCGCTCTGCGCGGAGGCTGCGCAGGGGCCAGCAGCCTGCCGACCTGCGGGAGGGTGTGGCCAAGGCCTACGACACAGTGCGAGAGGGCATCTTGGATACAGCTCAGACCATCTGTGACGTGGCATCGCGGGGCCATGAGCAGAAGGGGCTGACGGGCGCCGTGGGGGGCGTGATCCGCCAGCTGCCCCCGACTGTGGTGAAGCCGCTCATCCTGGCCACGGAGGCCACGTCCAGCCTGCTCGGGGGCATGCGCAACCAGATTGTCCCCGACGCCCACAAGGACCACGCCCTCAAGTGGCGCTCGGACAGTGCCCAAGACTGAGAATTC

Amino Acid Sequence

MEQKLISEEDLGSMSRWLWPWSNCVKERVCRYLLHHYLGHFFQEHLSLDQLSLDLYKGSVALRDIHLEIWSVNEVLESMESPLELVEGFVGSIEVAVPWAALLTDHCTVRVSGLQLTLQPRRGPAPGAADSQSWASCMTTSLQLAQECLRDGLPEPSEPPQPLEGLEMFAQTIETVLRRIKVTFLDTVVRVEHSPGDGERGVAVEVRVQRLEYCDEAVRDPSQAPPVDVHQPPAFLHKLLQLAGVRLHYEELPAQEEPPEPPLQIGSCSGYMELMVKLKQNEAFPGPKLEVAGQLGSLHLLLTPRQLQQLQELLSAVSLTDHEGLADKLNKSRPLGAEDLWLIEQDLNQQLQAGAVAEPLSPDPLTNPLLNLDNTDLFFSMAGLTSSVASALSELSLSDVDLASSVRSDMASRRLSAQAHPAGKMAPNPLLDTMRPDSLLKMTLGGVTLTLLQTSAPSSGPPDLATHFFTEFDATKDGPFGSRDFHHLRPRFQRACPCSHVRLTGTAVQLSWELRTGSRGRRTTSMEVHFGQLEVLECLWPRGTSEPEYTEILTFPGTLGSQASARPCAHLRHTQILRRVPKSRPRRSVACHCHSELALDLANFQADVELGALDRLAALLRLATVPAEPPAGLLTEPLPAMEQQTVFRLSAPRATLRLRFPIADLRPERDPWAGQAVRAEQLRLELSEPQFRSELSSGPGPPVPTHLELTCSDLHGIYEDGGKPPVPCLRVSKALDPKSTGRKYFLPQVVVTVNPQSSSTQWEVAPEKGEELELSVESPCELREPEPSPFSSKRTMYETEEMVIPGDPEEMRTFQSRTLALSRCSLEVILPSVHIFLPSKEVYESIYNRINNDLLMWEPADLLPTPDPAAQPSGFPGPSGFWHDSFKMCKSAFKLANCFDLTPDSDSDDEDAHFFSVGASGGPQAAAPEAPSLHLQSTFSTLVTVLKGRITALCETKDEGGKRLEAVHGELVLDMEHGTLFSVSQYCGQPGLGYFCLEAEKATLYHRAAVDDYPLPSHLDLPSFAPPAQLAPTIYPSEEGVTERGASGRKGQGRGPHMLSTAVRIHLDPHKNVKEFLVTLRLHKATLRHYMALPEQSWHSQLLEFLDVLDDPVLGYLPPTVITILHTHLFSCSVDYRPLYLPVRVLITAETFTLSSNIIMDTSTFLLRFILDDSALYLSDKCEVETLDLRRDYVCVLDVDLLELVIKTWKGSTEGKLSQPLFELRCSNNVVHVHSCADSCALLVNLLQYVMSTGDLHPPPRPPSPTEIAGQKLSESPASLPSCPPVETALINQRDLADALLDTERSLRELAQPSGGHLPQASPISVYLFPGERSGAPPPSPPVGGPAGSLGSCSEEKEDEREEEGDGDTLDSDEFCILDAPGLGIPPRDGEPVVTQLHPGPIVVRDGYFSRPIGSTDLLRAPAHFPVPSTRVVLREVSLVWHLYGGRDFGPHPGHRARTGLSGPRSSPSRCSGPNRPQNSWRTQGGSGRQHHVLMEIQLSKVSFQHEVYPAEPATGPAAPSQELEERPLSRQVFIVQELEVRDRLASSQINKFLYLHTSERMPRRAHSNMLTIKALHVAPTTNLGGPECCLRVSLMPLRLNVDQDALFFLKDFFTSLVAGINPVVPGETSAEARPETRAQPSSPLEGQAEGVETTGSQEAPGGGHSPSPPDQQPIYFREFRFTSEVPIWLDYHGKHVTMDQVGTFAGLLIGLAQLNCSELKLKRLCCRHGLLGVDKVLGYALNEWLQDIRKNQLPGLLGGVGPMHSVVQLFQGFRDLLWLPIEQYRKDGRLMRGLQRGAASFGSSTASAALELSNRLVQAIQATAETVYDILSPAAPVSRSLQDKRSARRLRRGQQPADLREGVAKAYDTVREGILDTAQTICDVASRGHEQKGLTGAVGGVIRQLPPTVVKPLILATEATSSLLGGMRNQIVPDAHKDHALKWRSDSAQD*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU59736
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: silent C645T, SNP P656R
• Low Temp: No