cDNA Clone FAM83H

• Plasmid: pBabeD puro GFP-FAM83H E694X
• Parent Plasmid: pBabeD puro GFP
• Expressed: GFP FAM83H E694X
• Genbank: NM_198488.3
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCATGGCCCGTCGCTCTCAGAGCTCCTCGCAGGGGGACAACCCACTGGCACCCGGGTACCTGCCGCCTCACTACAAAGAGTACTACCGCCTGGCGGTGGATGCACTGGCCGAGGGTGGCTCGGAGGCCTACAGCCGCTTCCTCGCTACCGAGGGGGCACCAGACTTCCTGTGCCCTGAAGAGCTGGAACATGTGAGCCGACACCTTCGGCCTCCGCAGTATGTTACCCGAGAGCCACCTGAAGGCAGCCTTCTCGACGTGGACATGGATGGCTCCTCGGGTACATACTGGCCAGTGAACTCAGACCAGGCCGTGCCTGAGCTTGATTTGGGCTGGCCTCTGACCTTCGGCTTCCAGGGCACCGAGGTGACCACCTTGGTGCAGCCACCGCCCCCCGACAGCCCCAGTATCAAGGATGAGGCCCGCAGGATGATCCGTTCCGCCCAGCAGGTGGTGGCCGTGGTGATGGACATGTTCACTGATGTGGACCTGCTCAGCGAAGTGCTGGAGGCCGCGGCCCGTCGGGTCCCAGTCTACATCCTGCTGGATGAGATGAACGCGCAGCACTTCCTGGACATGGCCGACAAGTGCCGTGTCAACCTGCACCACGTGGATTTCCTGCGCGTACGGACTGTGGCGGGCCCCACCTACTACTGCCGCACTGGGAAGTCCTTCAAGGGCCACGTCAAGGAGAAGTTCCTGCTGGTGGACTGTGCCGTGGTGATGAGTGGGAGCTACAGCTTCATGTGGTCCTTTGAGAAGATCCACCGCAGCCTGGCGCACGTGTTCCAAGGAGAGCTGGTCTCCAGCTTCGACGAGGAGTTCCGCATCCTCTTCGCGCAGTCCGAGCCGCTTGTGCCCTCGGCCGCGGCCCTGGCCCGCATGGACGCCTATGCCCTGGCTCCGTATGCCGGGGCCGGGCCTCTCGTGGGCGTCCCTGGGGTCGGGGCGCCAACCCCCTTCTCCTTCCCTAAACGAGCGCACCTCCTGTTCCCGCCACCCCGGGAAGAGGGCCTGGGCTTCCCCTCCTTCCTCGACCCGGACCGCCACTTCCTGTCGGCCTTCCGCCGGGAGGAGCCGCCGCGGATGCCGGGGGGCGCGCTGGAACCGCACGCGGGGCTGCGGCCACTCTCGCGGCGCCTGGAGGCCGAGGCCGGGCCGGCTGGGGAGCTCGCGGGCGCGCGGGGCTTCTTCCAGGCGCGGCACCTGGAGATGGACGCCTTCAAGCGGCACAGCTTCGCGACCGAGGGCGCGGGCGCCGTGGAGAACTTCGCGGCTGCGCGGCAGGTGTCGCGGCAGACGTTCCTCAGCCACGGCGACGACTTCCGCTTCCAGACCAGCCACTTCCACCGTGACCAGCTCTACCAGCAGCAGTACCAGTGGGACCCGCAGCTCACGCCGGCGCGCCCGCAAGGCCTGTTCGAGAAGCTTCGCGGGGGCCGCGCGGGTTTCGCGGACCCGGATGACTTCACCCTGGGCGCCGGGCCCCGCTTCCCGGAGCTCGGACCCGACGGGCACCAGCGGCTGGACTACGTGCCGTCCAGCGCGTCCCGCGAGGTGCGCCACGGCTCGGACCCCGCCTTCGCGCCCGGACCCCGCGGCCTGGAGCCCAGCGGAGCCCCGCGCCCCAACCTGACCCAGCGCTTCCCATGCCAGGCCGCGGCGAGGCCGGGCCCAGACCCCGCTCCCGAGGCGGAGCCGGAGCGCAGGGGCGGGCCCGAGGGGCGGGCAGGGCTGCGGCGCTGGCGTTTGGCCTCCTACTTGAGCGGCTGCCACGGCGAGGATGGGGGCGACGACGGCCTACCGGCGCCCATGGAAGCGGAGGCTTACGAAGACGACGTGCTGGCTCCCGGGGGCCGGGCACCTGCCGGCGACCTGCTCCCCTCGGCCTTCCGCGTCCCAGCAGCCTTCCCCACCAAGGTCCCGGTGCCAGGCCCGGGCAGCGGCGGCAACGGCCCAGAGCGCGAGGGCCCGGAGGAGCCTGGCCTGGCCAAGCAGGACTCATTCCGCTCGCGCCTGAACCCCCTGGTCCAGCGCAGCTCCAGGCTGCGCTCCTCGCTCATCTTCAGCACGTCACAGGCCTAGGGCGCGGCCGGGGCTGCGGCGGCCACTGAGAAGGTGCAGCTGCTGCACAAGGAGCAGACGGTCAGCGAGACGCTGGGGCCCGGCGGAGAGGCCGTGCGCTCCGCGGCTTCCACCAAGGTGGCGGAGCTGCTGGAGAAGTACAAGGGCCCAGCCCGTGATCCCGGCGGCGGCGCGGGCGCCATCACCGTTGCCAGCCACAGCAAGGCCGTCGTGTCCCAGGCGTGGCGGGAAGAGGTGGCGGCCCCAGGTGCCGTGGGGGGCGAGCGCCGCAGCCTCGAGAGCTGCCTGCTGGACCTGCGCGACTCCTTTGCACAGCAGCTGCACCAGGAGGCGGAGCGGCAGCCGGGAGCCGCGTCGCTCACCGCGGCGCAGCTGCTCGACACACTGGGCCGGAGCGGCTCCGACCGCCTGCCTTCCCGCTTCCTCTCTGCCCAGAGCCACTCAACGTCCCCGCAAGGGCTGGACAGCCCTCTGCCGCTGGAAGGGTCCGGAGCGCACCAGGTGCTCCATAATGAGTCAAAAGGGAGCCCCACCTCGGCTTACCCTGAGCGGAAGGGGAGCCCCACGCCTGGGTTTTCCACTCGAAGAGGAAGTCCAACTACAGGATTTATCGAGCAGAAGGGGAGCCCCACCTCAGCCTACCCCGAGCGCAGGGGTAGTCCGGTGCCCCCCGTGCCGGAGCGCAGGAGCAGTCCGGTGCCCCCCGTGCCGGAGCGCAGGGGCAGCCTCACCCTTACCATCTCCGGGGAGTCCCCGAAGGCCGGGCCCGCGGAGGAGGGGCCGAGCGGCCCCATGGAAGTCCTGCGCAAAGGCTCCTTGCGTCTTAGGCAGCTGCTGAGCCCCAAGGGCGAGCGGCGCATGGAGGATGAGGGTGGCTTCCCAGTGCCGCAGGAGAACGGCCAACCCGAGAGCCCGCGGCGTCTGTCACTGGGCCAGGGTGACAGCACGGAGGCTGCCACAGAAGAGCGGGGTCCGCGGGCGCGCCTGTCCTCAGCCACGGCCAACGCCTTGTACAGCAGCAACCTTCGGGATGACACGAAGGCCATTCTGGAGCAGATCAGTGCCCACGGCCAGAAGCACCGTGCGGTCCCTGCCCCGAGCCCCGGCCCGACCCACAACAGCCCCGAGCTAGGCCGTCCACCGGCTGCTGGCGTCCTGGCCCCAGATATGTCCGACAAGGACAAGTGTTCAGCCATCTTCCGCTCGGACAGCTTGGGGACCCAGGGCCGGCTGAGCCGCACGCTGCCAGCCAGCGCGGAGGAGCGCGATCGGCTGCTGCGCCGCATGGAGAGCATGCGCAAGGAGAAGCGCGTGTACAGCCGCTTCGAGGTCTTCTGCAAGAAAGAGGAGGCCAGCAGCCCTGGGGCAGGGGAAGGCCCCGCGGAGGAGGGCACCAGGGACAGCAAGGTGGGCAAGTTCGTGCCCAAGATCCTGGGCACGTTCAAAAGCAAGAAGTGAGCGGCCGC

Amino Acid Sequence

MHVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFIC
TTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTI
FFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSH
NVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDN
HYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMARRS
QSSSQGDNPLAPGYLPPHYKEYYRLAVDALAEGGSEAYSRFLATEGAPDF
LCPEELEHVSRHLRPPQYVTREPPEGSLLDVDMDGSSGTYWPVNSDQAVP
ELDLGWPLTFGFQGTEVTTLVQPPPPDSPSIKDEARRMIRSAQQVVAVVM
DMFTDVDLLSEVLEAAARRVPVYILLDEMNAQHFLDMADKCRVNLHHVDF
LRVRTVAGPTYYCRTGKSFKGHVKEKFLLVDCAVVMSGSYSFMWSFEKIH
RSLAHVFQGELVSSFDEEFRILFAQSEPLVPSAAALARMDAYALAPYAGA
GPLVGVPGVGAPTPFSFPKRAHLLFPPPREEGLGFPSFLDPDRHFLSAFR
REEPPRMPGGALEPHAGLRPLSRRLEAEAGPAGELAGARGFFQARHLEMD
AFKRHSFATEGAGAVENFAAARQVSRQTFLSHGDDFRFQTSHFHRDQLYQ
QQYQWDPQLTPARPQGLFEKLRGGRAGFADPDDFTLGAGPRFPELGPDGH
QRLDYVPSSASREVRHGSDPAFAPGPRGLEPSGAPRPNLTQRFPCQAAAR
PGPDPAPEAEPERRGGPEGRAGLRRWRLASYLSGCHGEDGGDDGLPAPME
AEAYEDDVLAPGGRAPAGDLLPSAFRVPAAFPTKVPVPGPGSGGNGPERE
GPEEPGLAKQDSFRSRLNPLVQRSSRLRSSLIFSTSQA*GAAGAAAATEK
VQLLHKEQTVSETLGPGGEAVRSAASTKVAELLEKYKGPARDPGGGAGAI
TVASHSKAVVSQAWREEVAAPGAVGGERRSLESCLLDLRDSFAQQLHQEA
ERQPGAASLTAAQLLDTLGRSGSDRLPSRFLSAQSHSTSPQGLDSPLPLE
GSGAHQVLHNESKGSPTSAYPERKGSPTPGFSTRRGSPTTGFIEQKGSPT
SAYPERRGSPVPPVPERRSSPVPPVPERRGSLTLTISGESPKAGPAEEGP
SGPMEVLRKGSLRLRQLLSPKGERRMEDEGGFPVPQENGQPESPRRLSLG
QGDSTEAATEERGPRARLSSATANALYSSNLRDDTKAILEQISAHGQKHR
AVPAPSPGPTHNSPELGRPPAAGVLAPDMSDKDKCSAIFRSDSLGTQGRL
SRTLPASAEERDRLLRRMESMRKEKRVYSRFEVFCKKEEASSPGAGEGPA
EEGTRDSKVGKFVPKILGTFKSKK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU29733
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Silent changes at Pro375 and Ala425 compared to NCBI ref. ; Parental 33932
• Low Temp: No