cDNA Clone KIAA1390 (MINDY1)

• Plasmid: pGEX6P1 KIAA1390 C137A F190A D196A R213A E221A Y222A L231A D230A N260A E363A 110-38
• Parent Plasmid: pGEX6P1
• Expressed: GST KIAA1390 C137A F190A D196A R213A E221A Y222A L231A D230A N260A E363A 110-38
• Tag: GST

Sequence of Insert

AGATCTATGGAGCCAGATTTCTACTGTGTCAAGTGGATCCCTTGGAAAGGAGAACAGACACCCATCATCACCCAGAGCACTAACGGCCCTGCCCCTCTCCTTGCCATCATGAACATCCTCTTTCTTCAGTGGAAGGTGAAGCTCCCCCCGCAGAAGGAAGTGATCACATCGGATGAGCTCATGGCCCATCTTGGAAACTGCCTCCTGTCCATCAAGCCCCAGGAGAAGTCAGAGGGACTTCAGCTTAATGCTCAGCAGAATGTGGATGCTGCAATGACAGTGCTGCCTAAACTGGCCACAGGTCTGGATGTCAATGTGGCATTCACAGGCGTCTCTGATTTTGCGGCTACACCCGAGTGCAGTGTCTTTGCCGCGCTAGGCATACCTCTGTACCATGGCTGGCTTGTTGATCCACAGAGTCCTGAGGCTGTGCGTGCAGTTGGGAAACTGAGTTACGCCCAGCTGGTGGCGAGGATCATCACCTGCAAACACTCCAGTGACACCAACCTCGTGACAGAAGGCCTGATTGCAGAGCAGTTCCTGGAGACCACCGCGGCCCAGCTGACCTACCACGGACTGTGTGAGCTGACAGCAGCTGCTAAGGAGGGTGAACTTAGCGTCTTTTTCCGAAACAACCACTTTAGCACCATGACTAAGCATAAGAGTCACTTATACCTACTGGTCACTGACCAGGGCTTTCTACAGGAGGAGCAAGTCGTATGGGAGAGCCTGCACAATGTGGATGGAGACAGCTGCTTTTGTGACTCTGACTTTCACCTGAGTCATTCCCTGGGCAAGGGGCCTGGAGCAGAAGGTGGGAGTGGCTCCCCAGAATAGGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMEPDFYCVKWIPWKGEQTPIITQSTNGPAPLLAIMNILFLQWKVKLPPQKEVITSDELMAHLGNCLLSIKPQEKSEGLQLNAQQNVDAAMTVLPKLATGLDVNVAFTGVSDFAATPECSVFAALGIPLYHGWLVDPQSPEAVRAVGKLSYAQLVARIITCKHSSDTNLVTEGLIAEQFLETTAAQLTYHGLCELTAAAKEGELSVFFRNNHFSTMTKHKSHLYLLVTDQGFLQEEQVVWESLHNVDGDSCFCDSDFHLSHSLGKGPGAEGGSGSPE*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU59648
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Please mutate C137 to alanine 59500
• Low Temp: No