cDNA Clone ZNF516

• Synonyms: HsT287
• Plasmid: pMK-RQ ZNF516 optimised Bam Not
• Parent Plasmid: pMK-RQ
• Genbank: NM_014643
• Species: Human

Sequence of Insert

GGATCCATGGACCGGAACAGAGAAGCCGAGATGGAACTGAGAAGAGGCCCATCTCCTACCAGAGCCGGAAGAGGCCATGAAGTGGACGGCGATAAGGCCACCTGTCACACCTGTTGTATCTGCGGCAAGAGCTTCCCGTTCCAGTCCAGCCTGAGCCAGCACATGAGAAAGCACACCGGCGAGAAGCCCTACAAGTGCCCCTACTGCGATCACAGAGCCAGCCAGAAGGGCAACCTGAAGATCCACATCAGAAGCCACCGGACCGGCACACTGATTCAGGGACATGAACCTGAGGCCGGCGAAGCCCCTCTGGGAGAAATGAGAGCTAGCGAAGGCCTGGATGCCTGCGCTAGCCCAACAAAATCTGCCAGCGCCTGCAACAGACTGCTGAATGGCGCTTCTCAGGCCGATGGCGCCAGAGTTCTTAATGGCGCTAGCCAGGCCGACAGCGGAAGAGTGCTGCTGAGAAGCTCTAAGAAGGGCGCCGAGGGATCTGCTTGTGCTCCTGGCGAAGCTAAAGCCGCCGTGCAGTGTAGCTTCTGCAAGAGCCAGTTCGAGCGGAAGAAGGACCTGGAACTGCACGTCCACCAGGCTCACAAGCCTTTCAAGTGCCGGCTGTGCAGCTACGCCACACTGAGAGAGGAAAGCCTGCTGAGCCACATCGAGCGGGATCACATCACAGCTCAAGGCCCTGGAAGCGGCGAGGCCTGTGTGGAAAATGGCAAGCCTGAACTGAGCCCTGGCGAGTTCCCTTGTGAAGTGTGCGGCCAGGCCTTTAGCCAGACCTGGTTTCTGAAGGCCCACATGAAGAAGCACCGGGGCAGCTTCGATCACGGCTGCCATATCTGTGGCAGACGGTTCAAAGAGCCATGGTTCCTGAAGAATCACATGAAGGCTCACGGCCCCAAGACCGGCAGCAAGAACAGACCTAAGAGCGAGCTGGACCCTATCGCCACCATCAACAACGTGGTGCAAGAGGAAGTGATCGTTGCCGGCCTGAGCCTGTACGAAGTCTGTGCCAAGTGCGGCAACCTGTTCACCAACCTGGACAGCCTGAACGCCCACAACGCCATCCACAGAAGAGTGGAAGCCAGCAGAACACGGGCCCCTGCTGAAGAAGGTGCCGAAGGACCTAGCGATACCAAGCAGTTCTTCCTGCAGTGCCTGAACCTGCGGCCTTCTGCTGCTGGCGATTCTTGTCCTGGAACACAGGCCGGAAGAAGAGTCGCCGAACTGGACCCCGTGAATAGCTATCAGGCTTGGCAGCTGGCCACCAGAGGCAAAGTTGCCGAGCCTGCCGAGTACCTGAAATACGGCGCTTGGGATGAAGCCCTGGCCGGCGACGTTGCCTTCGATAAGGATCGGAGAGAATACGTGCTGGTGTCCCAAGAGAAGCGCAAGAGAGAACAGGACGCTCCTGCTGCTCAGGGCCCTCCTAGAAAAAGAGCTTCTGGCCCAGGCGATCCTGCTCCTGCCGGACATCTTGATCCTAGATCTGCCGCCAGACCTAACAGAAGGGCCGCTGCTACAACAGGCCAGGGCAAGAGCAGCGAGTGCTTCGAGTGTGGCAAGATCTTCCGGACCTACCACCAGATGGTGCTGCACTCCAGAGTGCACCGCAGAGCCAGACGGGAAAGAGATTCTGACGGCGACAGAGCCGCTAGGGCCAGATGTGGTTCTCTGAGCGAAGGCGATAGCGCCAGCCAGCCTTCTTCTCCTGGTTCTGCATGTGCCGCCGCTGATAGCCCTGGATCTGGACTGGCTGATGAAGCCGCCGAGGATTCTGGCGAAGAAGGCGCTCCTGAACCAGCTCCAGGTGGACAGCCCAGAAGATGCTGCTTCAGCGAAGAAGTGACCAGCACCGAGCTGAGCAGCGGCGATCAGTCTCACAAGATGGGCGACAACGCCAGCGAGAGAGATACCGGCGAGTCTAAGGCCGGAATCGCCGCCTCTGTGTCCATCCTGGAAAACAGCAGCCGGGAAACCAGCCGCCGACAAGAACAGCACAGATTCAGCATGGATCTGAAGATGCCCGCCTTCCATCCTAAGCAAGAGGTGCCAGTTCCTGGCGACGGCGTGGAATTTCCAAGCTCTACAGGCGCCGAAGGCCAGACAGGACACCCTGCCGAAAAGCTGAGCGACCTGCACAACAAAGAGCACAGCGGCGGAGGCAAGAGAGCACTGGCTCCAGATCTGATGCCTCTGGACCTGAGCGCCAGAAGCACTAGGGACGACCCCAGCAACAAAGAAACAGCCAGCTCTCTGCAGGCCGCTCTGGTGGTTCACCCTTGTCCTTACTGCAGCCACAAGACCTACTATCCCGAGGTGCTGTGGATGCACAAGCGGATCTGGCACAGAGTGTCCTGCAATAGCGTGGCCCCTCCATGGATTCAGCCCAACGGCTACAAGAGCATCCGGTCCAACCTGGTGTTCCTGAGCAGAAGCGGAAGGACAGGACCACCTCCTGCTCTCGGCGGAAAAGAGTGTCAACCTCTGCTGCTCGCCCGGTTCACCAGAACACAAGTTCCAGGCGGCATGCCCGGCTCTAAGAGCGGATCTTCTCCACTGGGCGTCGTGACAAAGGCCGCCAGCATGCCCAAGAACAAAGAGAGCCATTCTGGCGGCCCTTGCGCTCTTTGGGCTCCTGGACCAGATGGCTACAGACAGACCAAGCCTTGCCACGGCCAAGAACCTCATGGCGCTGCAACACAAGGCCCTCTGGCCAAGCCTAGACAAGAGGCCAGCTCTAAGCCAGTGCCTGCTCCAGGCGGAGGCGGCTTTTCAAGAAGCGCTACCCCTACACCTACCGTGATCGCTAGAGCAGGCGCACAGCCTAGCGCCAATAGCAAGCCCGTGGAAAAGTTCGGAGTGCCTCCTGCTGGCGCCGGATTTGCCCCTACAAACAAACACAGCGCCCCAGATAGCCTGAAGGCCAAGTTTTCTGCCCAGCCACAGGGTCCTCCACCAGCTAAAGGCGAAGGCGGAGCACCTCCACTGCCTCCAAGAGAGCCTCCATCTAAGGCTGCCCAAGAGCTGAGAACCCTGGCCACATGTGCTGCCGGATCTAGAGGGGATGCTGCCCTGCAAGCTCAACCTGGCGTTGCAGGGGCTCCTCCTGTGCTGCATAGCATCAAGCAAGAGCCTGTGGCCGAGGGCCACGAGAAGAGACTGGATATCCTGAACATCTTCAAGACTTACATCCCCAAGGACTTCGCCACTCTGTACCAAGGCTGGGGAGTGTCTGGACCCGGCCTGGAACATAGAGGCACCCTGAGAACTCAGGCCAGACCAGGCGAGTTCGTGTGTATCGAGTGCGGAAAGTCCTTTCACCAGCCTGGCCATCTGAGAGCCCATATGAGAGCACACAGCGTGGTGTTCGAGAGCGACGGCCCTAGAGGTTCTGAGGTGCACACCACAAGCGCCGACGCTCCTAAGCAGGGCAGAGATCACAGCAATACCGGCACCGTGCAGACCGTGCCTCTGAGAAAGGGAACCTGAGCGGCCGC

Amino Acid Sequence

MDRNREAEMELRRGPSPTRAGRGHEVDGDKATCHTCCICGKSFPFQSSLSQHMRKHTGEKPYKCPYCDHRASQKGNLKIHIRSHRTGTLIQGHEPEAGEA
PLGEMRASEGLDACASPTKSASACNRLLNGASQADGARVLNGASQADSGRVLLRSSKKGAEGSACAPGEAKAAVQCSFCKSQFERKKDLELHVHQAHKPF
KCRLCSYATLREESLLSHIERDHITAQGPGSGEACVENGKPELSPGEFPCEVCGQAFSQTWFLKAHMKKHRGSFDHGCHICGRRFKEPWFLKNHMKAHGP
KTGSKNRPKSELDPIATINNVVQEEVIVAGLSLYEVCAKCGNLFTNLDSLNAHNAIHRRVEASRTRAPAEEGAEGPSDTKQFFLQCLNLRPSAAGDSCPG
TQAGRRVAELDPVNSYQAWQLATRGKVAEPAEYLKYGAWDEALAGDVAFDKDRREYVLVSQEKRKREQDAPAAQGPPRKRASGPGDPAPAGHLDPRSAAR
PNRRAAATTGQGKSSECFECGKIFRTYHQMVLHSRVHRRARRERDSDGDRAARARCGSLSEGDSASQPSSPGSACAAADSPGSGLADEAAEDSGEEGAPE
PAPGGQPRRCCFSEEVTSTELSSGDQSHKMGDNASERDTGESKAGIAASVSILENSSRETSRRQEQHRFSMDLKMPAFHPKQEVPVPGDGVEFPSSTGAE
GQTGHPAEKLSDLHNKEHSGGGKRALAPDLMPLDLSARSTRDDPSNKETASSLQAALVVHPCPYCSHKTYYPEVLWMHKRIWHRVSCNSVAPPWIQPNGY
KSIRSNLVFLSRSGRTGPPPALGGKECQPLLLARFTRTQVPGGMPGSKSGSSPLGVVTKAASMPKNKESHSGGPCALWAPGPDGYRQTKPCHGQEPHGAA
TQGPLAKPRQEASSKPVPAPGGGGFSRSATPTPTVIARAGAQPSANSKPVEKFGVPPAGAGFAPTNKHSAPDSLKAKFSAQPQGPPPAKGEGGAPPLPPR
EPPSKAAQELRTLATCAAGSRGDAALQAQPGVAGAPPVLHSIKQEPVAEGHEKRLDILNIFKTYIPKDFATLYQGWGVSGPGLEHRGTLRTQARPGEFVC
IECGKSFHQPGHLRAHMRAHSVVFESDGPRGSEVHTTSADAPKQGRDHSNTGTVQTVPLRKGT*

• Vector Type: Other
• Antibiotic: Kan
• Full Snapgene Map: pMK-RQ ZNF516 optimised Bam Not24.86 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU60977
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Holding vector for fragment. The native sequence of ZNF516 is particularly GC rich so the codon useage has been altered to improve stability and allow propagation at 37oC.
• Low Temp: No