cDNA Clone UBE2A

• Synonyms: UBC2, HHR6A, MRXSN, RAD6A, MRXS30, ubiquitin-conjugating enzyme E2 A
• Plasmid: pET28b-UBE2A isoform 1 C152S
• Parent Plasmid: pET28b original
• Expressed: 6His-UBE2A isoform 1 C152S
• Genbank: Gene ID 7319 NM_003336.3 → NP_003327.2 P49459
• Tag: 6His
• Species: Human

Sequence of Insert

GTCGACTCCACCCCGGCTCGGCGGCGCCTCATGCGGGACTTCAAGAGGTT
GCAGGAGGATCCTCCAGCCGGAGTCAGCGGGGCTCCGTCCGAGAACAACA
TAATGGTGTGGAACGCGGTCATTTTCGGGCCTGAAGGGACCCCGTTTGAG
GATGGAACATTTAAACTTACAATAGAATTCACTGAAGAATATCCAAATAA
ACCACCTACAGTTAGATTTGTCTCTAAGATGTTCCATCCAAATGTCTATG
CAGATGGTAGTATATGTCTGGACATACTTCAGAACCGTTGGAGTCCAACC
TATGATGTGTCTTCCATTCTAACATCCATACAGTCTCTGTTGGATGAACC
CAATCCCAATAGTCCAGCAAACAGCCAGGCTGCTCAGCTGTACCAGGAGA
ACAAACGGGAATATGAAAAGCGTGTTTCTGCAATAGTAGAACAAAGCTGG
CGTGATTcTTGAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRDPNSSSVDSTPARRRLMR
DFKRLQEDPPAGVSGAPSENNIMVWNAVIFGPEGTPFEDGTFKLTIEFTE
EYPNKPPTVRFVSKMFHPNVYADGSICLDILQNRWSPTYDVSSILTSIQS
LLDEPNPNSPANSQAAQLYQENKREYEKRVSAIVEQSWRDS*

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU29764
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: no native initiating methionine.; Ser41 of fusion = Ser2 of UBE2A
• Low Temp: No