cDNA Clone ZNF516

• Synonyms: HsT287
• Plasmid: pcDNA5D frtTO GFP ZNF516
• Parent Plasmid: pcDNA5D frtTO GFP
• Expressed: GFP-ZNF516
• Genbank: NM_014643
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCATGGACCGGAACAGAGAAGCCGAGATGGAACTGAGAAGAGGCCCATCTCCTACCAGAGCCGGAAGAGGCCATGAAGTGGACGGCGATAAGGCCACCTGTCACACCTGTTGTATCTGCGGCAAGAGCTTCCCGTTCCAGTCCAGCCTGAGCCAGCACATGAGAAAGCACACCGGCGAGAAGCCCTACAAGTGCCCCTACTGCGATCACAGAGCCAGCCAGAAGGGCAACCTGAAGATCCACATCAGAAGCCACCGGACCGGCACACTGATTCAGGGACATGAACCTGAGGCCGGCGAAGCCCCTCTGGGAGAAATGAGAGCTAGCGAAGGCCTGGATGCCTGCGCTAGCCCAACAAAATCTGCCAGCGCCTGCAACAGACTGCTGAATGGCGCTTCTCAGGCCGATGGCGCCAGAGTTCTTAATGGCGCTAGCCAGGCCGACAGCGGAAGAGTGCTGCTGAGAAGCTCTAAGAAGGGCGCCGAGGGATCTGCTTGTGCTCCTGGCGAAGCTAAAGCCGCCGTGCAGTGTAGCTTCTGCAAGAGCCAGTTCGAGCGGAAGAAGGACCTGGAACTGCACGTCCACCAGGCTCACAAGCCTTTCAAGTGCCGGCTGTGCAGCTACGCCACACTGAGAGAGGAAAGCCTGCTGAGCCACATCGAGCGGGATCACATCACAGCTCAAGGCCCTGGAAGCGGCGAGGCCTGTGTGGAAAATGGCAAGCCTGAACTGAGCCCTGGCGAGTTCCCTTGTGAAGTGTGCGGCCAGGCCTTTAGCCAGACCTGGTTTCTGAAGGCCCACATGAAGAAGCACCGGGGCAGCTTCGATCACGGCTGCCATATCTGTGGCAGACGGTTCAAAGAGCCATGGTTCCTGAAGAATCACATGAAGGCTCACGGCCCCAAGACCGGCAGCAAGAACAGACCTAAGAGCGAGCTGGACCCTATCGCCACCATCAACAACGTGGTGCAAGAGGAAGTGATCGTTGCCGGCCTGAGCCTGTACGAAGTCTGTGCCAAGTGCGGCAACCTGTTCACCAACCTGGACAGCCTGAACGCCCACAACGCCATCCACAGAAGAGTGGAAGCCAGCAGAACACGGGCCCCTGCTGAAGAAGGTGCCGAAGGACCTAGCGATACCAAGCAGTTCTTCCTGCAGTGCCTGAACCTGCGGCCTTCTGCTGCTGGCGATTCTTGTCCTGGAACACAGGCCGGAAGAAGAGTCGCCGAACTGGACCCCGTGAATAGCTATCAGGCTTGGCAGCTGGCCACCAGAGGCAAAGTTGCCGAGCCTGCCGAGTACCTGAAATACGGCGCTTGGGATGAAGCCCTGGCCGGCGACGTTGCCTTCGATAAGGATCGGAGAGAATACGTGCTGGTGTCCCAAGAGAAGCGCAAGAGAGAACAGGACGCTCCTGCTGCTCAGGGCCCTCCTAGAAAAAGAGCTTCTGGCCCAGGCGATCCTGCTCCTGCCGGACATCTTGATCCTAGATCTGCCGCCAGACCTAACAGAAGGGCCGCTGCTACAACAGGCCAGGGCAAGAGCAGCGAGTGCTTCGAGTGTGGCAAGATCTTCCGGACCTACCACCAGATGGTGCTGCACTCCAGAGTGCACCGCAGAGCCAGACGGGAAAGAGATTCTGACGGCGACAGAGCCGCTAGGGCCAGATGTGGTTCTCTGAGCGAAGGCGATAGCGCCAGCCAGCCTTCTTCTCCTGGTTCTGCATGTGCCGCCGCTGATAGCCCTGGATCTGGACTGGCTGATGAAGCCGCCGAGGATTCTGGCGAAGAAGGCGCTCCTGAACCAGCTCCAGGTGGACAGCCCAGAAGATGCTGCTTCAGCGAAGAAGTGACCAGCACCGAGCTGAGCAGCGGCGATCAGTCTCACAAGATGGGCGACAACGCCAGCGAGAGAGATACCGGCGAGTCTAAGGCCGGAATCGCCGCCTCTGTGTCCATCCTGGAAAACAGCAGCCGGGAAACCAGCCGCCGACAAGAACAGCACAGATTCAGCATGGATCTGAAGATGCCCGCCTTCCATCCTAAGCAAGAGGTGCCAGTTCCTGGCGACGGCGTGGAATTTCCAAGCTCTACAGGCGCCGAAGGCCAGACAGGACACCCTGCCGAAAAGCTGAGCGACCTGCACAACAAAGAGCACAGCGGCGGAGGCAAGAGAGCACTGGCTCCAGATCTGATGCCTCTGGACCTGAGCGCCAGAAGCACTAGGGACGACCCCAGCAACAAAGAAACAGCCAGCTCTCTGCAGGCCGCTCTGGTGGTTCACCCTTGTCCTTACTGCAGCCACAAGACCTACTATCCCGAGGTGCTGTGGATGCACAAGCGGATCTGGCACAGAGTGTCCTGCAATAGCGTGGCCCCTCCATGGATTCAGCCCAACGGCTACAAGAGCATCCGGTCCAACCTGGTGTTCCTGAGCAGAAGCGGAAGGACAGGACCACCTCCTGCTCTCGGCGGAAAAGAGTGTCAACCTCTGCTGCTCGCCCGGTTCACCAGAACACAAGTTCCAGGCGGCATGCCCGGCTCTAAGAGCGGATCTTCTCCACTGGGCGTCGTGACAAAGGCCGCCAGCATGCCCAAGAACAAAGAGAGCCATTCTGGCGGCCCTTGCGCTCTTTGGGCTCCTGGACCAGATGGCTACAGACAGACCAAGCCTTGCCACGGCCAAGAACCTCATGGCGCTGCAACACAAGGCCCTCTGGCCAAGCCTAGACAAGAGGCCAGCTCTAAGCCAGTGCCTGCTCCAGGCGGAGGCGGCTTTTCAAGAAGCGCTACCCCTACACCTACCGTGATCGCTAGAGCAGGCGCACAGCCTAGCGCCAATAGCAAGCCCGTGGAAAAGTTCGGAGTGCCTCCTGCTGGCGCCGGATTTGCCCCTACAAACAAACACAGCGCCCCAGATAGCCTGAAGGCCAAGTTTTCTGCCCAGCCACAGGGTCCTCCACCAGCTAAAGGCGAAGGCGGAGCACCTCCACTGCCTCCAAGAGAGCCTCCATCTAAGGCTGCCCAAGAGCTGAGAACCCTGGCCACATGTGCTGCCGGATCTAGAGGGGATGCTGCCCTGCAAGCTCAACCTGGCGTTGCAGGGGCTCCTCCTGTGCTGCATAGCATCAAGCAAGAGCCTGTGGCCGAGGGCCACGAGAAGAGACTGGATATCCTGAACATCTTCAAGACTTACATCCCCAAGGACTTCGCCACTCTGTACCAAGGCTGGGGAGTGTCTGGACCCGGCCTGGAACATAGAGGCACCCTGAGAACTCAGGCCAGACCAGGCGAGTTCGTGTGTATCGAGTGCGGAAAGTCCTTTCACCAGCCTGGCCATCTGAGAGCCCATATGAGAGCACACAGCGTGGTGTTCGAGAGCGACGGCCCTAGAGGTTCTGAGGTGCACACCACAAGCGCCGACGCTCCTAAGCAGGGCAGAGATCACAGCAATACCGGCACCGTGCAGACCGTGCCTCTGAGAAAGGGAACCTGAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMDRNREAEMELRRGPSPTRAGRGHEVDGDKATCHTCCICGKSFPFQSSLSQHMRKH
TGEKPYKCPYCDHRASQKGNLKIHIRSHRTGTLIQGHEPEAGEAPLGEMRASEGLDACASPTKSASACNRLLNGASQADGARVLNGASQADSGRVLLRSS
KKGAEGSACAPGEAKAAVQCSFCKSQFERKKDLELHVHQAHKPFKCRLCSYATLREESLLSHIERDHITAQGPGSGEACVENGKPELSPGEFPCEVCGQA
FSQTWFLKAHMKKHRGSFDHGCHICGRRFKEPWFLKNHMKAHGPKTGSKNRPKSELDPIATINNVVQEEVIVAGLSLYEVCAKCGNLFTNLDSLNAHNAI
HRRVEASRTRAPAEEGAEGPSDTKQFFLQCLNLRPSAAGDSCPGTQAGRRVAELDPVNSYQAWQLATRGKVAEPAEYLKYGAWDEALAGDVAFDKDRREY
VLVSQEKRKREQDAPAAQGPPRKRASGPGDPAPAGHLDPRSAARPNRRAAATTGQGKSSECFECGKIFRTYHQMVLHSRVHRRARRERDSDGDRAARARC
GSLSEGDSASQPSSPGSACAAADSPGSGLADEAAEDSGEEGAPEPAPGGQPRRCCFSEEVTSTELSSGDQSHKMGDNASERDTGESKAGIAASVSILENS
SRETSRRQEQHRFSMDLKMPAFHPKQEVPVPGDGVEFPSSTGAEGQTGHPAEKLSDLHNKEHSGGGKRALAPDLMPLDLSARSTRDDPSNKETASSLQAA
LVVHPCPYCSHKTYYPEVLWMHKRIWHRVSCNSVAPPWIQPNGYKSIRSNLVFLSRSGRTGPPPALGGKECQPLLLARFTRTQVPGGMPGSKSGSSPLGV
VTKAASMPKNKESHSGGPCALWAPGPDGYRQTKPCHGQEPHGAATQGPLAKPRQEASSKPVPAPGGGGFSRSATPTPTVIARAGAQPSANSKPVEKFGVP
PAGAGFAPTNKHSAPDSLKAKFSAQPQGPPPAKGEGGAPPLPPREPPSKAAQELRTLATCAAGSRGDAALQAQPGVAGAPPVLHSIKQEPVAEGHEKRLD
ILNIFKTYIPKDFATLYQGWGVSGPGLEHRGTLRTQARPGEFVCIECGKSFHQPGHLRAHMRAHSVVFESDGPRGSEVHTTSADAPKQGRDHSNTGTVQT
VPLRKGT*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pcDNA5D frtTO GFP ZNF51624.86 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU64018
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: The native sequence of ZNF516 is particularly GC rich so the codon useage has been altered to improve stability. May be grown at 37oC though 30oC is recommended.
• Low Temp: No