cDNA Clone EXO5

• Synonyms: C1orf176; DEM1; Exo V; hExo5
• Plasmid: pMEX3Cb EXO5 delta D19-E24
• Parent Plasmid: pMEX3Cb
• Expressed: MBP TEV 3C EXO5 delta D19-E24
• Genbank: NM_022774.2
• Tag: MBP
• Species: Human

Sequence of Insert

GGATCCATGGCAGAGACAAGAGAAGAGGAGACAGTGTCAGCAGAAGCCTCAGGGTTCTCATTCCTGGAGTTTCTGGACCTAGAAGATGCCCAAGAGTCAAAGGCTTTAGTTAACATGCCTGGCCCATCTTCTGAATCCCTTGGGAAGGATGACAAACCCATAAGCTTACAAAACTGGAAAAGAGGATTGGATATATTATCACCCATGGAGAGATTCCACCTTAAATATTTATATGTCACTGACCTGGCTACTCAGAACTGGTGTGAACTGCAAACAGCATATGGGAAGGAGCTTCCTGGTTTCTTGGCACCTGAGAAGGCAGCTGTGTTGGACACTGGTGCCAGCATACACCTAGCTAGAGAACTAGAACTTCATGATCTTGTGACTGTCCCAGTCACCACTAAAGAAGATGCTTGGGCAATTAAGTTTCTGAACATACTTTTGCTGATTCCTACCCTGCAGTCAGAAGGGCACATCAGAGAGTTTCCAGTGTTTGGGGAAGTGGAGGGTGTACTTCTTGTTGGAGTGATTGATGAGCTGCACTATACAGCCAAGGGGGAACTGGAGCTGGCGGAACTCAAGACACGCAGGCGCCCTATGCTCCCTCTGGAAGCTCAGAAGAAGAAAGACTGTTTTCAAGTCAGCCTATACAAATATATCTTTGATGCCATGGTACAAGGAAAAGTGACCCCTGCTAGCCTAATCCACCACACAAAGTTGTGTCTAGAAAAGCCACTGGGGCCATCAGTGCTGAGGCATGCCCAGCAGGGAGGCTTCTCTGTGAAGTCTTTGGGTGACCTCATGGAACTTGTCTTCTTGTCTCTAACACTGTCAGACCTCCCAGTTATTGATATCTTGAAGATTGAGTATATCCACCAAGAGACTGCCACTGTGCTGGGTACTGAGATTGTAGCCTTCAAAGAGAAGGAGGTGAGAGCCAAGGTGCAGCATTATATGGCCTACTGGATGGGCCACCGAGAGCCCCAGGGAGTTGACGTGGAGGAGGCTTGGAAGTGCCGGACGTGTACCTATGCAGACATTTGTGAGTGGAGAAAGGGCAGTGGAGTGCTCAGCTCTACACTGGCGCCCCAAGTCAAAAAAGCCAAATGAGCGGCCGC

Amino Acid Sequence

MMKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVR
YNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDL
IKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKP
LGAVALKSYEEELVKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNNNNNNNNLGENLYFQGGSLEVLFQG
PLGSMAETREEETVSAEASGFSFLEFLDLEDAQESKALVNMPGPSSESLGKDDKPISLQNWKRGLDILSPMERFHLKYLYVTDLATQNWCELQTAYGKEL
PGFLAPEKAAVLDTGASIHLARELELHDLVTVPVTTKEDAWAIKFLNILLLIPTLQSEGHIREFPVFGEVEGVLLVGVIDELHYTAKGELELAELKTRRR
PMLPLEAQKKKDCFQVSLYKYIFDAMVQGKVTPASLIHHTKLCLEKPLGPSVLRHAQQGGFSVKSLGDLMELVFLSLTLSDLPVIDILKIEYIHQETATV
LGTEIVAFKEKEVRAKVQHYMAYWMGHREPQGVDVEEAWKCRTCTYADICEWRKGSGVLSSTLAPQVKKAK*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pMEX3Cb EXO5 delta D19-E2463.65 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU61048
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: DLSDSE region (aa19-24) removed. Compared to reference sequence there is one SNP at G172V.
• Low Temp: No