cDNA Clone DCAF1

• Synonyms: RIP; VPRBP
• Plasmid: pcDNA5 FRT/TO GFP NLS DCAF1
• Parent Plasmid: pcDNA5 FRT/TO GFP NLS
• Expressed: GFP NLS DCAF1
• Genbank: NM_014703.2 with delta A87 (seen in isoform 2)
• Tag: GFP
• Species: Human

Sequence of Insert

GCGGCCGCGATGACTACAGTAGTGGTACATGTGGACTCCAAAGCTGAGCTCACTACCCTGCTGGAGCAGTGGGAAAAGGAACATGGCAGTGGGCAGGACATGGTACCTATCCTTACCAGGATGTCTCAATTGATTGAAAAAGAAACTGAAGAGTATCGTAAAGGGGATCCAGACCCATTTGATGATCGACATCCTGGTCGAGCTGATCCAGAGTGTATGCTGGGCCACTTGCTGAGAATACTCTTCAAGAATGATGATTTCATGAATCTGGTGAATGCATATGTGATGACAAGCCGAGAGCCCCCTTTAAACACTGCAGCTTGCAGACTCCTATTAGACATCATGCCAGGGCTGGAAACTGCTGTCGTCTTTCAAGAAAAGGAGGGAATTGTCGAGAATCTTTTCAAATGGGCCCGAGAGGCCGATCAACCATTGAGGACATATTCTACTGGACTGTTAGGAGGTGCTATGGAAAATCAAGACATTGCTGCCAACTATAGAGATGAAAATTCACAGCTGGTGGCAATAGTGCTTCGAAGACTGAGGGAGCTACAGCTACAGGAAGTGGCTTTGCGGCAGGAAAACAAGCGTCCCAGTCCACGGAAGCTCTCTTCTGAACCCCTTTTGCCTCTGGATGAGGAGGCTGTGGATATGGACTATGGTGACATGGCTGTAGATGTAGTGGATGGAGACCAAGAGGAAGCTTCTGGAGACATGGAGATCTCCTTTCATCTTGATTCAGGCCACAAGACTAGTAGCAGAGTGAACTCAACAACCAAACCTGAGGATGGAGGATTAAAGAAAAACAAGTCAGCAAAACAGGGTGACAGAGAGAACTTTAGGAAAGCCAAGCAAAAGTTGGGTTTCTCATCTTCTGATCCAGATCGCATGTTTGTTGAGCTGTCTAATAGCAGTTGGTCAGAAATGTCTCCCTGGGTGATTGGCACCAATTATACCCTTTATCCTATGACTCCTGCTATCGAGCAGCGACTCATTCTCCAATATTTGACCCCTCTAGGAGAATATCAGGAGCTACTTCCCATATTCATGCAACTTGGATCACGGGAGCTGATGATGTTCTATATTGACCTGAAGCAAACTAATGATGTCCTGCTTACATTTGAGGCACTAAAGCACCTAGCATCTCTCCTGCTCCATAACAAATTTGCCACAGAATTTGTTGCGCATGGTGGAGTACAGAAATTACTGGAAATACCTCGTCCTTCTATGGCTGCAACCGGTGTGTCTATGTGTTTGTATTACCTATCCTACAATCAGGATGCCATGGAAAGAGTTTGCATGCATCCCCACAATGTTCTGTCTGATGTGGTGAACTATACCCTGTGGTTAATGGAGTGTTCTCATGCTTCAGGATGCTGCCATGCTACCATGTTTTTTTCAATTTGCTTCTCATTTCGGGCCGTCTTGGAGCTCTTTGACCGCTATGATGGTCTTCGTCGTCTGGTGAACTTGATCAGTACTTTGGAGATTCTAAATTTGGAAGATCAGGGTGCACTTCTGAGTGATGATGAAATATTTGCTAGCCGCCAAACTGGGAAACATACCTGCATGGCCTTGCGCAAATACTTTGAGGCTCACCTGGCCATTAAATTGGAACAAGTGAAGCAGTCACTTCAGAGGACTGAGGGTGGCATTCTTGTCCACCCACAACCCCCGTACAAGGCATGCTCATATACTCATGAACAGATTGTGGAAATGATGGAATTTTTGATAGAATATGGCCCAGCGCAGCTATATTGGGAACCAGCTGAAGTTTTCCTCAAACTTTCTTGTGTGCAACTCTTGTTGCAGCTTATTTCTATTGCCTGCAATTGGAAGACCTATTATGCAAGGAATGACACTGTGCGCTTTGCTTTGGATGTCCTGGCTATTCTTACTGTGGTGCCAAAAATCCAGCTCCAGTTGGCAGAATCAGTGGACGTGTTGGATGAGGCTGGATCTACAGTCTCTACTGTAGGTATCAGCATTATTTTGGGAGTGGCTGAGGGTGAGTTCTTCATCCATGATGCTGAAATTCAGAAGTCAGCACTTCAGATTATCATCAATTGTGTGTGTGGCCCAGATAACCGAATATCCAGTATTGGTAAATTTATCTCTGGTACTCCTCGGAGAAAGCTGCCTCAGAACCCTAAAAGCAGTGAGCACACCCTGGCCAAGATGTGGAATGTGGTTCAGTCCAACAACGGCATCAAGGTGCTCCTGTCCTTACTGTCCATTAAGATGCCCATCACAGATGCAGACCAAATCCGGGCCCTGGCCTGCAAAGCCCTAGTGGGCCTGTCTCGCAGTAGCACTGTCCGGCAGATCATCAGTAAACTGCCCCTTTTCAGCAGCTGCCAGATCCAGCAGCTGATGAAGGAGCCTGTGCTGCAGGACAAGCGCAGTGACCATGTCAAGTTCTGCAAGTATGCTGCTGAACTCATTGAACGGGTGTCAGGAAAACCACTTCTCATTGGCACTGATGTTTCCCTAGCACGACTGCAGAAAGCAGATGTTGTTGCCCAGTCAAGGATCTCCTTCCCTGAGAAAGAGCTGCTTTTGTTGATACGAAACCATCTTATTTCTAAAGGGCTTGGAGAAACAGCAACCGTGCTGACAAAAGAGGCTGACCTGCCCATGACTGCTGCCTCCCATTCTTCTGCCTTTACCCCAGTCACTGCTGCTGCTTCTCCTGTCTCTCTACCCCGAACCCCTCGTATCGCTAATGGCATTGCAACTCGTCTGGGCAGCCATGCTGCTGTGGGTGCCTCTGCGCCTTCTGCCCCTACTGCTCATCCTCAGCCACGGCCCCCCCAGGGTCCGCTAGCTCTGCCCGGCCCATCTTATGCAGGCAACTCCCCTTTGATTGGTAGAATCAGTTTTATCAGAGAGAGGCCATCACCCTGCAATGGCAGGAAAATCAGAGTGTTGCGGCAGAAGTCGGACCATGGTGCCTACAGCCAAAGCCCAGCCATAAAAAAACAGCTGGACAGACATCTTCCTTCCCCACCTACGCTGGACAGTATAATCACAGAGTATCTTAGAGAACAACATGCTCGCTGCAAGAATCCAGTTGCCACCTGCCCACCTTTCTCCCTCTTTACTCCTCACCAATGTCCTGAGCCAAAACAGAGGCGGCAAGCGCCAATAAACTTTACGTCAAGGCTAAACCGCAGGGCATCATTTCCAAAGTATGGAGGGGTGGATGGCGGATGCTTTGATAGGCACCTTATCTTTAGCAGATTCCGTCCTATTTCAGTGTTCCGGGAAGCCAATGAAGATGAGAGTGGCTTCACCTGCTGTGCATTCTCAGCACGGGAGCGGTTCCTGATGCTTGGCACCTGCACAGGGCAGCTGAAGCTCTATAATGTGTTTAGTGGACAGGAGGAGGCCAGCTATAACTGTCACAACTCAGCCATCACACATCTTGAACCTTCCAGGGATGGGTCCTTGCTGCTGACATCTGCTACTTGGAGCCAGCCTTTGTCTGCACTTTGGGGAATGAAGTCAGTATTTGATATGAAGCATTCCTTCACAGAAGATCACTATGTTGAGTTCAGTAAGCACTCCCAGGATCGGGTCATCGGCACAAAAGGAGACATTGCCCACATTTATGATATTCAGACTGGCAACAAGCTGTTGACTCTGTTTAACCCAGATCTTGCCAACAACTACAAGAGGAACTGTGCCACCTTTAATCCTACAGATGATCTTGTCTTAAATGATGGCGTCCTCTGGGATGTCCGCTCTGCACAGGCCATCCACAAGTTTGACAAGTTCAATATGAACATCAGTGGTGTTTTCCATCCAAATGGACTGGAGGTGATCATTAATACTGAGATTTGGGACCTTCGAACTTTTCATCTTTTGCATACTGTTCCCGCTCTGGATCAGTGTCGCGTGGTGTTCAATCACACGGGAACAGTGATGTATGGAGCTATGTTGCAGGCAGATGATGAAGATGACTTAATGGAAGAGAGGATGAAAAGCCCCTTTGGGTCATCCTTCCGAACATTTAATGCAACTGACTACAAACCTATAGCAACCATTGATGTGAAACGGAACATCTTTGACCTGTGTACAGACACCAAAGACTGCTATCTTGCTGTCATTGAGAATCAAGGCAGCATGGATGCCCTGAACATGGACACAGTATGCAGGCTGTATGAAGTGGGCAGGCAGCGTCTGGCAGAGGATGAGGATGAAGAGGAGGACCAGGAAGAGGAAGAACAGGAGGAAGAAGATGATGATGAAGATGATGATGACACCGATGATTTAGATGAGCTTGACACTGACCAGTTGCTGGAGGCGGAGTTGGAGGAGGACGACAATAATGAGAACGCAGGGGAAGATGGGGACAATGACTTCTCTCCCTCTGATGAGGAGCTAGCAAACCTTCTAGAGGAGGGAGAGGACGGGGAGGATGAAGACTCTGATGCAGATGAGGAGGTGGAACTGATCCTGGGGGACACTGACAGCTCTGACAACTCTGATTTGGAAGATGACATCATCTTATCTCTGAATGAGTGAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLPPKKKRKVGSSRKGTIDAAAMTTVVVHVDSKAELTTLLEQWEKEHGSGQDMVPILTRM
SQLIEKETEEYRKGDPDPFDDRHPGRADPECMLGHLLRILFKNDDFMNLVNAYVMTSREPPLNTAACRLLLDIMPGLETAVVFQEKEGIVENLFKWAREA
DQPLRTYSTGLLGGAMENQDIAANYRDENSQLVAIVLRRLRELQLQEVALRQENKRPSPRKLSSEPLLPLDEEAVDMDYGDMAVDVVDGDQEEASGDMEI
SFHLDSGHKTSSRVNSTTKPEDGGLKKNKSAKQGDRENFRKAKQKLGFSSSDPDRMFVELSNSSWSEMSPWVIGTNYTLYPMTPAIEQRLILQYLTPLGE
YQELLPIFMQLGSRELMMFYIDLKQTNDVLLTFEALKHLASLLLHNKFATEFVAHGGVQKLLEIPRPSMAATGVSMCLYYLSYNQDAMERVCMHPHNVLS
DVVNYTLWLMECSHASGCCHATMFFSICFSFRAVLELFDRYDGLRRLVNLISTLEILNLEDQGALLSDDEIFASRQTGKHTCMALRKYFEAHLAIKLEQV
KQSLQRTEGGILVHPQPPYKACSYTHEQIVEMMEFLIEYGPAQLYWEPAEVFLKLSCVQLLLQLISIACNWKTYYARNDTVRFALDVLAILTVVPKIQLQ
LAESVDVLDEAGSTVSTVGISIILGVAEGEFFIHDAEIQKSALQIIINCVCGPDNRISSIGKFISGTPRRKLPQNPKSSEHTLAKMWNVVQSNNGIKVLL
SLLSIKMPITDADQIRALACKALVGLSRSSTVRQIISKLPLFSSCQIQQLMKEPVLQDKRSDHVKFCKYAAELIERVSGKPLLIGTDVSLARLQKADVVA
QSRISFPEKELLLLIRNHLISKGLGETATVLTKEADLPMTAASHSSAFTPVTAAASPVSLPRTPRIANGIATRLGSHAAVGASAPSAPTAHPQPRPPQGP
LALPGPSYAGNSPLIGRISFIRERPSPCNGRKIRVLRQKSDHGAYSQSPAIKKQLDRHLPSPPTLDSIITEYLREQHARCKNPVATCPPFSLFTPHQCPE
PKQRRQAPINFTSRLNRRASFPKYGGVDGGCFDRHLIFSRFRPISVFREANEDESGFTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAIT
HLEPSRDGSLLLTSATWSQPLSALWGMKSVFDMKHSFTEDHYVEFSKHSQDRVIGTKGDIAHIYDIQTGNKLLTLFNPDLANNYKRNCATFNPTDDLVLN
DGVLWDVRSAQAIHKFDKFNMNISGVFHPNGLEVIINTEIWDLRTFHLLHTVPALDQCRVVFNHTGTVMYGAMLQADDEDDLMEERMKSPFGSSFRTFNA
TDYKPIATIDVKRNIFDLCTDTKDCYLAVIENQGSMDALNMDTVCRLYEVGRQRLAEDEDEEEDQEEEEQEEEDDDEDDDDTDDLDELDTDQLLEAELEE
DDNNENAGEDGDNDFSPSDEELANLLEEGEDGEDEDSDADEEVELILGDTDSSDNSDLEDDIILSLNE*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pcDNA5 FRT/TO GFP NLS DCAF1889.09 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU63640
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Low Temp: No