cDNA Clone DCAF13

• Synonyms: GM83; Sof1; WDSOF1; HSPC064
• Plasmid: pcDNA5 FRT/TO GFP NLS DCAF13
• Parent Plasmid: pcDNA5 FRT/TO GFP NLS
• Expressed: GFP NLS DCAF13
• Genbank: NM_015420.7
• Tag: GFP
• Tag: NLS
• Species: Human

Sequence of Insert

AGATCTATGCAGTGGCCGCCACCGTGGCGACGGTCGCCCCTTGTGAGCGCAACCCCACCTCCGGGACCAACGAGAGGACTCTTATGCCCAAGGCGCGTGAGCGAAGCCGGAGACTCTAGTACTGAGGGGGCAAGAACGGGGCACAGACACTACGTCACGCCGGCGGAAAGCGCGATGGAGGGGAGGTCCCAGCCTCTCCCGAGTCTCCGCCCTGCCTCGCCCACAAATGCTCCAGCCCACGGACGCGGAAGCGACTGCACAGCTAAAGAACCGCCCCTTGCTAACGTTTGCCGGCAAAACTACGGAGCGGCGCAGGATCCAGTTGAGCCAGCAGGCCGCCGCTCCGCGAGTCACGTGACTGGAAGCAGTCTGGGAAAAGCGGAAGTCGCCTGTGGGAGGAGGTGGCGGTGGGCGGAACTCCTAGCGGACACCTCGTGGAGTCCGGCCGGAAGAGCAACCGAGATGAAGGTGAAGATGCTGAGCCGGAATCCGGACAATTATGTCCGCGAAACCAAGTTGGACTTACAGAGAGTTCCAAGAAACTATGATCCTGCTTTACATCCTTTTGAGGTCCCACGAGAATATATAAGAGCTTTAAATGCTACCAAACTGGAACGAGTATTTGCAAAACCATTCCTTGCTTCGCTGGATGGTCACCGTGATGGAGTCAACTGCTTGGCAAAGCATCCAGAGAAGCTGGCTACTGTCCTTTCTGGGGCGTGTGATGGAGAGGTTAGAATTTGGAATCTAACTCAGCGGAATTGTATCCGTACAATACAAGCACATGAAGGCTTTGTACGAGGAATATGTACTCGCTTTTGTGGGACTTCTTTTTTCACTGTTGGTGATGACAAAACTGTGAAGCAGTGGAAAATGGATGGGCCAGGCTATGGAGACGAGGAAGAGCCATTACATACAATATTAGGAAAGACAGTGTATACTGGGATTGATCATCACTGGAAAGAAGCTGTTTTTGCCACATGTGGACAGCAAGTAGACATTTGGGATGAACAAAGAACTAATCCTATATGTTCAATGACCTGGGGATTTGACAGTATAAGTAGTGTTAAATTTAACCCAATTGAGACATTTCTCTTGGGAAGTTGTGCATCTGACAGGAATATAGTACTGTACGATATGAGGCAAGCTACTCCTTTGAAAAAGGTTATCTTAGATATGAGAACAAATACAATCTGTTGGAACCCTATGGAAGCTTTCATTTTTACAGCAGCAAATGAAGATTATAACTTATATACTTTTGATATGCGTGCACTGGACACTCCTGTAATGGTCCATATGGATCATGTATCTGCAGTGCTTGATGTGGATTACTCTCCCACTGGGAAGGAGTTTGTGTCTGCTAGTTTCGATAAATCTATTCGAATCTTTCCTGTAGACAAAAGTCGAAGCAGGGAGGTATATCATACAAAGAGAATGCAACATGTTATCTGTGTAAAATGGACTTCTGACAGCAAGTATATTATGTGTGGATCTGATGAAATGAACATTCGCCTGTGGAAAGCTAATGCTTCTGAAAAATTGGGTGTGCTTACATCACGAGAAAAAGCAGCCAAGGATTATAACCAGAAATTGAAGGAGAAATTTCAGCATTATCCTCATATAAAACGTATAGCTCGTCATCGACATCTACCAAAATCTATCTATAGCCAGATTCAGGAACAGCGCATCATGAAAGAAGCTCGTCGACGAAAGGAAGTGAATCGTATTAAACACAGCAAGCCTGGATCTGTGCCACTTGTGTCAGAGAAGAAGAAACACGTAGTGGCAGTTGTAAAATAAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLPPKKKRKVGSMQWPPPWRRSPLVSATPPPGPTRGLLCPRRVSEAGDSSTEGARTGHRH
YVTPAESAMEGRSQPLPSLRPASPTNAPAHGRGSDCTAKEPPLANVCRQNYGAAQDPVEPAGRRSASHVTGSSLGKAEVACGRRWRWAELLADTSWSPAG
RATEMKVKMLSRNPDNYVRETKLDLQRVPRNYDPALHPFEVPREYIRALNATKLERVFAKPFLASLDGHRDGVNCLAKHPEKLATVLSGACDGEVRIWNL
TQRNCIRTIQAHEGFVRGICTRFCGTSFFTVGDDKTVKQWKMDGPGYGDEEEPLHTILGKTVYTGIDHHWKEAVFATCGQQVDIWDEQRTNPICSMTWGF
DSISSVKFNPIETFLLGSCASDRNIVLYDMRQATPLKKVILDMRTNTICWNPMEAFIFTAANEDYNLYTFDMRALDTPVMVHMDHVSAVLDVDYSPTGKE
FVSASFDKSIRIFPVDKSRSREVYHTKRMQHVICVKWTSDSKYIMCGSDEMNIRLWKANASEKLGVLTSREKAAKDYNQKLKEKFQHYPHIKRIARHRHL
PKSIYSQIQEQRIMKEARRRKEVNRIKHSKPGSVPLVSEKKKHVVAVVK*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pcDNA5 FRT/TO GFP NLS DCAF13165.66 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU61062
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Two silent changes compared to reference sequence at S120 (t360c) and N222 (t666c).
• Low Temp: No